EMAGE mouse gene expression spatial database

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EMAGE:17275

Pdia6 protein disulfide isomerase associated 6 ( MGI:1919103)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_008885_06	euxassay_008885_07	euxassay_008885_08	euxassay_008885_09	euxassay_008885_10

euxassay_008885_11	euxassay_008885_12	euxassay_008885_13	euxassay_008885_14	euxassay_008885_15

euxassay_008885_16	euxassay_008885_17	euxassay_008885_18	euxassay_008885_19	euxassay_008885_20

euxassay_008885_21	euxassay_008885_22	euxassay_008885_23	euxassay_008885_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17275_wholemount_strong.wlz

17275_wholemount_moderate.wlz

17275_wholemount_weak.wlz

17275_wholemount_possible.wlz

17275_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17275_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5689
Entity Detected:	Pdia6, protein disulfide isomerase associated 6 ( MGI:1919103)
Sequence:	sense strand is shown >T5689 GGCACGGACAGAATGCGGGTGATCGGCATGGCTCGCCTGGTGCTCGGTCTGGTGAGCTGCACCTTCTTTC TAGCAGTCAGCGGTCTGTATTCTTCTAGTGATGATGTCATCGAGTTGACGCCATCAAATTTCAACAGAGA AGTTATTCAGAGTGACGGCCTGTGGCTTGTAGAATTTTATGCACCATGGTGTGGTCACTGCCAGAGGTTA ACACCAGAATGGAAGAAAGCAGCAACGGCTTTGAAAGATGTTGTTAAAGTCGGGGCCGTCAATGCAGACA AACATCAGTCCCTGGGAGGTCAGTATGGTGTCCAGGGATTTCCTACCATCAAGATATTTGGAGCTAACAA AAACAAGCCAGAAGATTATCAGGGTGGCAGAACGGGAGAAGCCATTGTAGATGCCGCCCTCAGTGCCTTG CGCCAGCTCGTCAAGGATCGCCTTGGTGGGCGGAGTGGTGGGTACAGTTCTGGAAAGCAGGGCAGAGGTG ATAGTTCAAGTAAGAAAGACGTGGTAGAGCTGACGGATGACACCTTTGATAAGAATGTCCTTGACAGTGA AGACGTTTGGATGGTTGAGTTTTATGCTCCGTGGTGTGGACACTGCAAAAACCTGGAGCCAGAATGGGCT GCTGCGGCTACAGAAGTAAAGGAGCAAACAAAGGGAAAAGTGAAACTGGCAGCTGTGGATGCCACCGTGA ATCAGGTTCTGGCCAGCCGATACGGGATTAAAGGATTCCCTACAATCAAGATATTTCAGAAAGGCGAGTC TCCTGTGGACTATGATGGTGGTCGGACGAGATCTGACATAGTGTCAAGGGCCCTCGATTTGTTCTCTGAT AATGCCCCTCCTCCAGAGCTCCTTGAGATAATCAACGAAGACATAGCCAAGAAGACATGCGAGGAGCACC AGCTCTGTGTCGTGGCCGTACTGCCTCACATCCTGGACACTGGAGCTGCAGGCAGAAACTCTTACTTGGA AGTTCTTCTGAAACTGGCTGACAAGTACAAGAAGAAGATGTGGGGGTGGCTGTGGACAGAAGCTGGAGCC CAGTATGAACTGGAGAACGCACTAGGGATTGGAGGGTTCGGGTACCCTGCCATGGCAGCCATCAATGCAC GCAAGATGAAATTTGCTCTTCTCAAGGGGTCTTTCAGTGAACAAGGCATCAATGAGTTTCTCAGGGAACT GTCTTTCGGGCGTGGCTCCACAGCACCCGTAGGGGGTGGTTCCTTTCCTACCATCACTCCCAGGGAGCCC TGGGATGGCAAGGATGGTGAGCTCCCTGTGGAGGACGACATTGACCTCAGTGACGTGGAGCTGGATGACC TGGAGAAGGATGAGTTGTGAGCCACGGCCCAGCCTTCCTTGGGCTTTCCTTGGGAGCGAGCAGTTTTCCA GCAGTGAAAGTTCTTGCGGGCAGCTGTCTGCCGCTGGCCTTTCTGAGCAGCACATGCGGCCACTGGAAAC TGCCAACAGTGAACCTCAGCGTCTCAAGAAAACACTGACGAATTCTATGAATTGTAGCAGTGAATTGGAT TGTATTCTCTGGCATACTTTGAAGAAAACTGGGCTATTGAAACATTTTTCCCTCCTGACTGCTGCTTGAA TGTTCCTGGAGGCTGTTTCTTATGTATAGGGTTTTTAAAATGTGATTCCTTTGTTTGAATATTAATGGCT TTTTCCATTAAAGAATAAAATGATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2645183 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2645183 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17273 same embryo
	EMAGE:17272 same embryo
	EMAGE:17271 same embryo
	EMAGE:17276 same embryo
	EMAGE:17274 same embryo
	EurExpress:euxassay_008885 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source