EMAGE mouse gene expression spatial database

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EMAGE:17360

Pcolce2 procollagen C-endopeptidase enhancer 2 ( MGI:1923727)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003749. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003749_01	euxassay_003749_02	euxassay_003749_03	euxassay_003749_04

euxassay_003749_05	euxassay_003749_06	euxassay_003749_07	euxassay_003749_08	euxassay_003749_09

euxassay_003749_10	euxassay_003749_11	euxassay_003749_12	euxassay_003749_13	euxassay_003749_14

euxassay_003749_15	euxassay_003749_16	euxassay_003749_17	euxassay_003749_18	euxassay_003749_19

euxassay_003749_20	euxassay_003749_21	euxassay_003749_22	euxassay_003749_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17360_wholemount_strong.wlz

17360_wholemount_moderate.wlz

17360_wholemount_weak.wlz

17360_wholemount_possible.wlz

17360_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17360_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 11 18 19 20 21 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 22 weak expression: see section 23

hand mesenchyme

strong

regional

strong expression: see section 01 02 03 21 22 23

foot mesenchyme

strong

regional

strong expression: see section 06 07 08 22 23

leg

strong

regional

strong expression: see section 01 02 03 04 moderate expression: see section 05 06 21 22 23

thymus primordium

moderate

regional

moderate expression: see section 09 10 13 weak expression: see section 11 12

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

otic capsule

strong

regional

strong expression: see section 01 02 03 20 21 22 23 moderate expression: see section 04 18 19 weak expression: see section 05 06 15 16 17

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 14 15 16

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 09 10 11 14 15 16

meckel's cartilage

strong

regional

strong expression: see section 15 16 17 19 20 21 22 moderate expression: see section 03 04 05 06 07 13 14

lower jaw incisor

moderate

regional

moderate expression: see section 08 09 10 11 13 14

lower jaw molar

strong

regional

strong expression: see section 16 17 18 19 moderate expression: see section 06 07

upper jaw incisor

moderate

regional

moderate expression: see section 09 10 13 14

upper jaw molar

strong

regional

strong expression: see section 16 17 18 19 moderate expression: see section 06 07

trachea

weak

regional

weak expression: see section 11 12

axial skeleton

strong

regional

strong expression: see section 09 moderate expression: see section 07 08 10 11 12 13 weak expression: see section 14 15 16

basisphenoid bone

strong

regional

strong expression: see section 01 02 18 19 23

frontal bone primordium

strong

regional

strong expression: see section 01 02

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

viscerocranium

strong

regional

Expression in the turbinate bone.

clavicle

strong

regional

strong expression: see section 07 08 15 moderate expression: see section 16 17

axial skeleton tail region

moderate

regional

moderate expression: see section 14 15 16 weak expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7743
Entity Detected:	Pcolce2, procollagen C-endopeptidase enhancer 2 ( MGI:1923727)
Sequence:	sense strand is shown >T7743 TGCGGGCGCCCGGCGGGATGCTGCAGTGAGCTGCCGGCCATGGGGGGCGCGAGCGCCTGCATCCCGCTCT GCCTGCTTCTGGCCACCGCGCGGATGGCCCGGCCGCAGACGCCAGAGAGACCTGTTTTCACATGTGGCGG CATTCTTACCGGAGAGTCTGGATTTATTGGCAGTGAAGGTTTTCCTGGAATGTACCCTCCGAATAGCAAA TGTACTTGGAAAATCACAGTTCCTGAGGGCAAAGTGGTCGTCTTGAATTTTCGATTCATAGACCTGGAGA ATGACAACCTGTGTCGCTATGACTTCGTGGATGTGTACAATGGCCACGCCAATGGCCAGCGTATCGGGCG CTTCTGTGGCACATTCCGACCTGGATCGCTCGTAGCCAGCGGCAACAAGATGACGGTACAAATGATTTCT GACGCCAACACCGCGGGAAGTGGCTTCATGGCAACGTACTCTGCCGCTGCGCCAGATGGAAAAGGAGACC GGTATTGTGGAGGACGCCTTGAAAAACCTTCCGGTACCTTTAAAACTCCCAACTGGCCAGACCGAGATTA CCCCGTGGGAGTTACTTGTGTGTGGCACATCATAGCTCCGAAGAACCAGCTTATAGAGTTAAAGTTTGAG AAGTTCGATGTTGAGCGCGATAACTACTGCAGATACGACTACGTGGCTGTGTTTAACGGTGGGGAAGTCA ATGACGCCAAAAGAATTGGGAAGTACTGTGGGGACAGTCCACCTGTGCCGATCGTGTCTGAGAGAAATGA ACTTCTTATTCAGTTCTTATCAGACTTAAGTTTAACAGCGGATGGTTTTATTGGTCACTACAAATTCAGG CCGAAAAAGTTTCCCACAACCACCACCACGCCCGTCACCACCACACTCCCCGTAACTACAGGTCTAAAGC CCACTGTGGCCCTGTGTCAGCAGAAGTGTAGACGGATGGGGACTCTGGAGAGCAATTACTGTTCAAGCAA CTTCGTGTTAGCCGGCACAGTTATCACGACCGTCACTCGAGGTGGGAGCTTGCACGCCACAGTCTCGATC ATCAGCATCTACAGGGAAGGCAACCTGGCCATTCAGCAGGCTGGCAAGAACATGAGTGTCAAGCTCACTG TGGTGTGCAGGCAGTGCCCCCTTCTCAGACGAGGTCTCAATTACATTATTATGGGACAAGTGGGTGAAGA TGGGCGTGGCAAAATCATGCCAAACAGCTTTGTCAAAATGTTCAAGAATAAGAATCAGAAGCCCATGAAC GCACTGAAGAACAAGCAGTGTTAGCCTGAGCTCTGTCACATAACCTGAACTTACTCATCACCGCTGAACG ATCTGTCCACGTTAGGAAAAAAAAAAAAAAAAAACCACGCTATTTAATGCTGAGCGTTCTGAGAGATGGA GCTGAGTAACTCCTCACGCGATGGATGTGTGAGCCACGCCCCCAGTTCACTGGTGGAAGGTCTGCGCCTG CGCTGTGATCTCCAACATCTGCGAACTCACCCACCCAGTGCCTGATGGGGAAGAGCAGCGAGCTCCGACA TCTGGAAGCTTGAACATACATCTCTGTAGGAGGATATTCTAGAGTCGAGTTGTATGAAGATATACAAAGG ATTTTATAAAGTGCAATATTTATAATGATATTTGGTTACCTTCAAGCATTTGCTCTGAGGTGTCACATTC TTTTTTTCTCTTTCATTTTTTTAAAAGTCAATGCTTAATAAAATATTTTAAATGATAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6310294 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6310294 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17362 same embryo
	EMAGE:17359 same embryo
	EMAGE:17358 same embryo
	EMAGE:17363 same embryo
	EMAGE:17361 same embryo
	EurExpress:euxassay_003749 same experiment
	MGI:4827099 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source