EMAGE mouse gene expression spatial database

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EMAGE:17361

Smarcal1 SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 ( MGI:1859183)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003825. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003825_01	euxassay_003825_02	euxassay_003825_03	euxassay_003825_04

euxassay_003825_05	euxassay_003825_06	euxassay_003825_07	euxassay_003825_08	euxassay_003825_09

euxassay_003825_10	euxassay_003825_11	euxassay_003825_12	euxassay_003825_13	euxassay_003825_14

euxassay_003825_15	euxassay_003825_16	euxassay_003825_17	euxassay_003825_18	euxassay_003825_19

euxassay_003825_20	euxassay_003825_21	euxassay_003825_22	euxassay_003825_23	euxassay_003825_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17361_wholemount_strong.wlz

17361_wholemount_moderate.wlz

17361_wholemount_weak.wlz

17361_wholemount_possible.wlz

17361_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17361_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7009
Entity Detected:	Smarcal1, SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 ( MGI:1859183)
Sequence:	sense strand is shown >T7009 CAGGTCTCAGCAAAGAGGAATGTCTATGTGGTGGTGTGCATTTGTGGTTTATGGTTTATTAGTTGGACCT CTCATTCCGTGGTCTCATCTCCCCAACTGGGTCAGTCAGCATACCAGGCCTTGCTTTTACCAACTTCCCA ACTAAAGGGTGACATCCCTGCATGAGCCTATCCTGTTAAAAATGTCCTTGCCACTTACAGAGGAGCAGAG GAAAAAGATTGAAGAGAATCGGCAAAAAGCTTTGGCCCGTCGAGCTGAGAAGTTGTCAGAACAGCCACAG AGCACAGCTTCAGGCTCCTCTGCTGCCGGCCCGTCCCAGTCCAAGCAGGGCTCTCTCCTGAATCTCCTAG CGGAGCCTTCAAAGCCAGTGGGCCATGCCTCCATTTTCAAGCAACAGAATCTCAGTAATTCACTTCCTAC TGATCAGAGACCTCACAGCTCCCGCTGTTCTCAGCCCAGCCCAGCAGAGGAAACGACGGGGCTATGGAAG ACCCAAGGAGAGATGTCCACAGCCTGCCCAAAGCCTAACCCAAGTCCTCCAGGAGCCTCTAACCAACCAC TTTTGGGTTATAAATCCAGCGAGGGTCAACCCCAGGCAACCTGGGACACAGGAGCTTCCTCTTCTGGACC ATTCCCCAGGGACCCCGAGTTAGAGGCCAAGGCAGCAAGGCCTTCCACCTCTAGGCAGAGCATTTCGGAC AGCTTTTATGTGTTAGGGGGAAAAACACCTAGGACAGAAGGGAGACCCCCAAACATACTGCAAACCACAC CCCAAAACACAGGCTTTCTACGGGGAGCGTGCATAAAGACTGGGGATCGCTTCCGGGTAAAGATCGGCTA CAACCAAGAGCTCATTGCCGTGTTTAAGTCTCTGCCCAGCAGACACTATGATTCTTTTACAAAGACCTGG GACTTCAGCATGAGCGACTACAGAGCCTTGATGAAGGCAGTCGAGCGACTCTCCACGGTCTCCCTGAAGC CACTGGACGAGGCTGGTGGCAGTGTTGGAGGACAGACCAGCCTTCCCTCAGCTCCATCCCTGACCTTTGT GACAGGGAAGTGCATGCTCATCTCTCGAGTCCGCTTCGAGGTTGACATCGGCTATTCGGAGGCGGTGATT GGCCTGTTTAAACAGATGGAGTCCAGGAGTTACGATATCAAGACCAGGAAGTGGAGTTTTCTCTTGGAAG AGCACAATAAACTAATTGCAAGGTCACGTGAACTGAAGCAAGTTCAGCTGGACCCACTGCCCAAGACAGT GACCCTGGCATTTGCATCTCAGCTGGAGAAAACGTCTCCCAAACTGAAAGCAGACGTGCCAGAAGCGGAC CTTTCCGGAGTGGATGCCAAGCTTGTGTCTAGCCTCATGCCCTTTCAGAGGGAGGGAGTTAGCTTTGCCA TATCAAAAAGAGGCCGCCTGCTACTTGCCGATGACATGGGCCTAGGGAAGACCGTCCAAGCCATCTGCAT AGCAGCTTTTTACCGAAAGGAATGGCCACTGCTGGTGGTCGTGCCTTCCTCTGTGCGCTTCACCTGGGAG CAGGCCTTTCTTCGGTGGCTTCCATCTCTTAGCCCAGAGAACATCAACGTGGTGGTGACCGGGAAGGGCC GCCTAACAGCTGGCTTGGTCAATATTGTCAGCTTTGACCTCCTTTGCAAGTTGGAGAGGCAGCTAAAAAC CCCTTTCAAAGTCGTCATTATTGATGAATCACACTTCCTCAAGAACATAAAGACTGCCCGCTGCCGAGCA GCTGTGCCTATCCTAAAGGTTGCCAAGAGGGTGATCCTACTGTCAGGCACACCCGCCATGTCGCGGCCCG CAGAGCTCTACACACAGATCATTGCTGTCAAGCCAACCTTCTTCCCTCAGTTTCATGCCTTTGGACTCCG CTACTGTGACGCCAAACGGCTCCCTTGGGGCTGGGACTACTCAGGCTCCTCCAACCTGGGCGAGCTGAAG CTGCTGCTAGAGGAAGCAATCATGCTGCGGCGCCTCAAATCCGACGTGCTCTCCCAGCTTCCGGCCAAGC AGCGCAAGATGGTGGTGGTCAACCCTGGTCGGATCAGCTCCAGGGCTAAGGCAGCCCTGGATGCAGCAGC CAAAGAAATGACCAAGGACAAAACTAAACAGCAACAGAAAGAAGCTCTCCTTGTCTTCTTCAACAGGACA GCAGAAGCTAAAATCCCATGTGTCGTCGAGTATATCCTGGACCTTCTGGACAGCGGACGAGAGAAGTTTC TAGTGTTTGCACACCATAAGGTGATTCTGGATGCAGTTGCCAAGGAGCTCGAGAGGAAGAACGTTCAACA CATCCGGATCGATGGCTCCACTCCCTCAGCAGACCGGGAGGCTCAGTGCCAGCGGTTCCAGCTGTCCAAA GGGCACACAGTGGCACTGTTGTCCATCACAGCCGCCAACATGGGCCTCACCTTCTCCACAGCTGATCTGG TCGTGTTTGCTGAGCTCTTCTGGAACCCAGGGGTGCTGATCCAGGCCGAGGACCGTGTGCACCGCATTGG ACAGACAAACTCTGTGAGCATCCACTACCTGGTGGCAAAAGGCACAGCAGATGACTACCTTTGGCCTCTG ATTCAAGAGAAGATTAAAGTTCTGGGGGAAGCTGGGCTTTCTGAGACCAATTTCTCGGAAATGACGGAAG CTACAGACTACGTGCACAAGGACCCAAAGCAGAAGACGATCTATGACCTGTTCCAGCAGTCCTTTGAAGA CGATGGAAATGACATGGAATTCCTGGAAGCAGCAGAGTCCTTTGAGCTAGGAAGTACTTCAGGAACCTCT GGAAACATTTCCCAGGATCTGGGAGATCTCTTAGATGAAGACGAAGGCAGTCCGCCAAAGAAAAGCAGAT TTGAATTCTTTGATAATTGGGACAGCTTTTCCTCTCCCTTTTGACAGGGTCAGAAAAGGAAAGAAACGAA AGAAAGCAGTCACAAAATCATGGGGCTCATTGAAATAAAGTATTTTCTTTAAAAAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:3992325 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3992325 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17360 same embryo
	EMAGE:17362 same embryo
	EMAGE:17359 same embryo
	EMAGE:17358 same embryo
	EMAGE:17363 same embryo
	EurExpress:euxassay_003825 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source