EMAGE mouse gene expression spatial database

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EMAGE:17401

Atp6v0a4 ATPase, H+ transporting, lysosomal V0 subunit A4 ( MGI:2153480)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003829. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003829_01	euxassay_003829_02	euxassay_003829_03	euxassay_003829_04

euxassay_003829_05	euxassay_003829_06	euxassay_003829_07	euxassay_003829_08	euxassay_003829_09

euxassay_003829_10	euxassay_003829_11	euxassay_003829_12	euxassay_003829_13	euxassay_003829_14

euxassay_003829_15	euxassay_003829_16	euxassay_003829_17	euxassay_003829_18	euxassay_003829_19

euxassay_003829_20	euxassay_003829_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17401_wholemount_strong.wlz

17401_wholemount_moderate.wlz

17401_wholemount_weak.wlz

17401_wholemount_possible.wlz

17401_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17401_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

naris

moderate

regional

moderate expression: see section 12 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 15 16 17

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 13 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7794
Entity Detected:	Atp6v0a4, ATPase, H+ transporting, lysosomal V0 subunit A4 ( MGI:2153480)
Sequence:	sense strand is shown >T7794 CGCAGGATAAGTGGAAGGAGGTGAGGACCAGGCTGAAGAGGAGGGCGGCCTGCGGGGCAGAGGATGTGTT GATACAACAGAGTGGCCTCTGGAGAGGGAGCCAGGACAGTGTGTGAAGGGGGAGAGGCTGGGCAGCCTCC CTGCCACCGGGCCCTGGACCACTTGAAAGCCACAACTGACTGTGGCCACTTGCTGCTGCTACTGCTTCTG CTTCTGCTGTGCCCTGCTCCAGGAATCAGGCAGTGGGTCCCCCACACAGAACAAGCTCCAAGGAAACTCA GACTAAGGCTGGACCAACATGGCATCTGTGTTTCGAAGTGAGGAGATGTGTTTATCCCAAGTGTTTCTCC AGGTGGAGGCTGCGTATTGCTGTGTAGCTGAGCTGGGAGAGCTTGGCTTGGTTCAGTTCAAAGATCTAAA TGCAAATGTGAACAGCTTCCAGAGGAAGTTTGTGAATGAAGTCCGAAGGTGTGAGTCACTGGAGAGAATC CTGCGTTTTCTGGAAGATGAGATGCAAAACGAGATTTTAATCCAAGTGCCTGAGAAGGATGCTGAGACCC CTCTCCCTCGGGAAATGATCACCCTGGAGACGACTCTAGAGAAGTTGGAAGGAGAGCTGCAGGAAGCCAA CCAGAGCCACCAGGCTTTGAAGAAGAGCTTTCTGGAACTGACAGAGCTCAAATACCTCCTAAAGAAAACC CAGGACTTTTTTGAGACAGAAACCAACTTAGGTGAAGATTTCTTTGTTGAAGACACTTCGGGTCTCTTGG AGCTAAGAACCATACCTGCCTTCATGACTGGAAAGTTGGGGTTCACGGCTGGTGTGATAAACAGGGAGAG GATGGCTTCCTTCGAGAGGCTGCTGTGGAGAGTTTGCCGGGGAAACGTGTACTTGAAGTTCAGTGAAATG GACACACTTCTGGAGGACCCTGTGACGAAAGAAGAAATTAAAAAGAATATATTCATCATATTTTACCAAG GAGAACAGCTCAGGCTGAAAATCAAGAAGATCTGTGACGGGTTTCGTGCTACCATCTACCCCTGCCCAGA GCATGCAGCAGAGCGCAGAGAGATGCTGACCAGTGTCAACGTGAGGCTGGAAGACTTAATCACCGTCATT ACCCAAACAGAGTCTCACCGACAGCGCCTGCTGCAGGAAGCAGCTGCCAACTGGCACTCCTGGGTCATCA AAGTACAGAAGATGAAGGCCGTCTACCATGTGCTGAACATGTGCAACATTGACGTCACCCAGCAGTGCAT CATTGCCGAGATCTGGTTCCCAGTAGCAGACACTAGACACATTAAGAAGGCCCTGGAGCAAGGCATGGAA CTCAGTGGCTCCTCCATGATTCCCATCATGACAGAAGTGGAAACTAAAACAGACCCTCCCACCTTCAACA GGACGAATAAATTTACAGCTGGCTTCCAGAACATTGTTGATGCGTATGGTGTAGGTAGCTACCGAGAGAT AAACCCAGCTCCCTACACTATCATCACCTTCCCCTTCCTGTTCGCTGTGATGTTTGGAGACTGTGGTCAT GGAATGGTGATGCTGATGGCAGCCTTGTGGATGGTCCTAAATGAGAGGCACTTGCTGGCTCAGAAGAGCA CCAACGAGATGTGGAACATCTTCTTCAATGGGCGCTATTTGATCCTGCTCATGGGTATCTTCTCCATCTA CACCGGCTTGATCTACAACGACTGTTTCTCCAAATCTTTTAACATCTTTGGCTCCTCTTGGAGTGTCCAG CCTATGTTCAGAAACGGCACGTGGAATACTCACATCGTGGAAAACAGTCCATATTTGCAGCTGGACCCAG CCATCCCAGGAGTGTATTCTGGGAATCCCTACCCATTTGGAATTGATCCGATTTGGAACCTGGCTTCAAA CAAGCTTACATTTTTAAACTCCTATAAGATGAAGATGTCAGTCATCCTGGGAATCGCTCATATGATTTTT GGTGTTATTCTCAGCCTCTTCAATCACATTTACTTCAGAAGAACCCTCAACATCATTCTGCAATTCATTC CCGAGATGATTTTTATGCTGAGTCTGTTTGGATACCTGGTTTTCATGATCATTTTCAAGTGGTGCCGATA TGATGCCCACACATCCCGGAAGGCTCCTAGCATCCTCATCCACTTTATCGGCATGTTTCTGTTCGACTAC GATGACTCTTCCAACGCACCCCTGTATGGACATCAGCAAGAAGTCCAGACTTTCTTTGTCATCATAGCGC TGGTGTCTGTGCCATGGATGCTTTTGATTAAGCCATTTGTTCTTAGAGCCAAGCACCAGAAATCTCAGCT GCAGTCTTTCACGATCCACGAGGATGCTGTGGAGGGTGACCACTCGGGCCACTCTTCTAAGAAGACTGCA GGTGCCCATGGGATGAAGGACGGCCATGAGGAGGAGTTCAACTTTGGAGACATCTTTGTCCACCAAGCCA TTCACACCATTGAGTACTGCCTAGGCTGCATCTCTAATACAGCCTCGTACCTTCGGCTCTGGGCCCTCAG CTTGGCTCACGCTGAGCTGTCCGAGGTGCTCTGGACCATGGTCATGAGCATCGGCCTTCGCCTTCAAGGC TGGGCAGGGCTCGTTGGTGTCTTCATCATTTTTGCGGTCTTCGCCGTCCTCACCGTGGCCATCCTCCTTG TCATGGAGGGTCTCTCAGCCTTTCTGCACGCCCTCCGGCTGCATTGGGTGGAATTCCAGAACAAGTTCTA TGAGGGGGCTGGCTCCAAGTTCTCCCCCTTCTCCTTTAAGCACGTCCTGGAAGGCACAGCTGAGGAGTAG GTCTGTCTGTATCCTGGTGCAGGCGACAGCTGAAGAAGCTCTCACTCTTGTCTCTGACATCAGCCCAGGC CAGGTGTCCCATGCTGCAGTCCTTCTGAATGGCTGTCTGCAGTGTGAAGCTGTGTATGGTGCAGTATTTA ACAGGACTTAGCCACCTAACTGAGGGGCTACTTTGGTGAATCATTCGGACTTCATGTGTAGTCTTCATGA TCAAATACTAAAATAACTGGGAAACGGGGGGGGGGGGGGGGGCGGACATGACACTTGACCACCATCTTCC TGTGATAGTTTATTTTTATTTTACTTCCTTTCTGTTTGAGACAAGGTCTCTCTAGCTTTAACTGTTCTGG AAATAACCACACGGCCAAGGATGTCCTTGACTTTGAACTCAGGTTCCTCCTGCCTCTACTTCCCAAATGC TGAGATGACAGGCAGGAACCCCCTCACCTGAAGTTCCTGCAGTATTTCAAGTGTTATAATCATGAAAATA AATGTCTGCTTTATTCACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6315485 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6315485 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17404 same embryo
	EMAGE:17403 same embryo
	EMAGE:17402 same embryo
	EMAGE:17400 same embryo
	EurExpress:euxassay_003829 same experiment
	MGI:4823319 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source