EMAGE mouse gene expression spatial database

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EMAGE:17403

Kirrel2 kin of IRRE like 2 (Drosophila) ( MGI:2442334)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003831. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003831_01	euxassay_003831_02	euxassay_003831_03	euxassay_003831_04

euxassay_003831_05	euxassay_003831_06	euxassay_003831_07	euxassay_003831_08	euxassay_003831_09

euxassay_003831_10	euxassay_003831_11	euxassay_003831_12	euxassay_003831_13	euxassay_003831_14

euxassay_003831_15	euxassay_003831_16	euxassay_003831_17	euxassay_003831_18	euxassay_003831_19

euxassay_003831_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17403_wholemount_strong.wlz

17403_wholemount_moderate.wlz

17403_wholemount_weak.wlz

17403_wholemount_possible.wlz

17403_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17403_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15

diencephalon

moderate

regional

moderate expression: see section 09 10 11 12

telencephalon

moderate

regional

moderate expression: see section 10

4th ventricle

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata

moderate

regional

moderate expression: see section 09 10 11 12 13

metencephalon

moderate

regional

moderate expression: see section 09 10 11

midbrain

moderate

regional

moderate expression: see section 10 11

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 13 14

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17 18 19

metanephros

weak

single cell

weak expression: see section 04 05 14 15 16

testis

weak

single cell

weak expression: see section 03 04 05 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7792
Entity Detected:	Kirrel2, kin of IRRE like 2 (Drosophila) ( MGI:2442334)
Sequence:	sense strand is shown >T7792 GGTCCGGAATTCCCGGGATGAGAGGGGCTCCAGTCCAGCGTGGTGTGAGAGGCGTGCTATCAAGAAAGAA GTTGGAGGGGAACCAGTGCAACCCTAACTCTGCGAGATCTTGGGGTACACACACTCGGGATGCTGGCCTC CGCCCTCCTCGTTTTCCTTTGCTGTTTCAAAGGACATGCAGGCTCATCGCCCCATTTCCTACAACAGCCA GAGGACATGGTGGTGCTGTTGGGGGAGGAAGCCCGGCTGCCCTGCGCTCTGGGCGCGTACAGGGGGCTCG TGCAGTGGACTAAGGATGGGCTGGCTCTAGGGGGCGAAAGAGACCTTCCAGGGTGGTCCCGGTACTGGAT ATCGGGGAATTCAGCCAGTGGCCAGCATGACCTCCACATTAAGCCTGTGGAATTGGAAGATGAGGCATCG TATGAGTGCCAGGCTTCGCAAGCAGGTCTCCGATCACGACCAGCCCAACTGCACGTGATGGTCCCCCCAG AAGCTCCCCAGGTACTAGGCGGCCCCTCTGTGTCTCTGGTTGCTGGAGTTCCTGGAAATCTGACCTGTCG GAGTCGTGGGGATTCACGACCTGCCCCTGAACTACTGTGGTTCCGAGATGGGATCCGGCTGGATGGGAGC AGCTTCCACCAGACCACGCTGAAGGACAAGGCCACTGGAACAGTGGAAAACACCTTATTCCTGACCCCTT CCAGTCATGATGATGGTGCCACCTTGATCTGCAGAGCGCGAAGCCAGGCCCTGCCCACAGGGAGGGACAC AGCTGTTACACTGAGCCTTCAGTATCCCCCAATGGTGACTCTGTCTGCTGAGCCCCAGACTGTGCAGGAG GGAGAGAAGGTGACTTTCCTGTGTCAAGCCACTGCCCAGCCTCCTGTCACTGGCTACAGGTGGGCGAAGG GGGGATCCCCGGTGCTTGGGGCACGTGGGCCAAGGTTGGAGGTCGTTGCAGATGCCACTTTCCTGACTGA GCCGGTGTCCTGCGAGGTCAGCAACGCGGTCGGAAGCGCCAACCGCAGCACCGCGCTGGAAGTGTTGTAT GGACCCATTCTGCAGGCAAAACCTAAGTCCGTGTCCGTGGACGTGGGGAAAGATGCCTCCTTCAGCTGTG TCTGGCGCGGGAACCCACTTCCACGGATAACCTGGACCCGCATGGGTGGCTCTCAGGTGCTGAGCTCCGG GCCCACGCTGCGGCTTCCGTCCGTGGCACTGGAGGATGCGGGCGACTATGTATGCAGGGCTGAGCCGAGG AGAACGGGTCTGGGAGGCGGCAAAGCGCAGGCGAGGCTGACTGTGAACGCACCCCCTGTAGTGACAGCCC TGCAACCTGCACCAGCCTTTCTGAGGGGTCCTGCTCGCCTCCAGTGTGTGGTGTTTGCCTCCCCTGCCCC AGACTCGGTGGTTTGGTCTTGGGACGAGGGCTTCTTGGAGGCAGGCTCACTGGGCAGGTTCCTAGTGGAA GCCTTCCCAGCCCCGGAAGTGGAGGGGGGACAGGGCCCTGGCCTTATTTCTGTGCTACACATTTCCGGAA CCCAGGAGTCCGACTTTACCACCGGCTTCAACTGCAGTGCCCGCAACCGGCTAGGAGAGGGACGAGTCCA GATCCACTTGGGCCGTAGAGACTTGCTGCCTACTGTCCGGATTGTGGCTGGTGCAGCATCTGCAGCCACC TCTCTCCTTATGGTCATCACTGGAGTGGTCCTCTGCTGCTGGCGCCATGGCTCTCTCTCTAAGCAAAAGA ACTTGGTCCGGATCCCAGGAAGCAGCGAGGGTTCCAGTTCACGTGGCCCTGAGGAGGAGACAGGCAGCAG TGAGGACCGGGGTCCCATTGTGCACACCGACCACAGTGATTTGGTTCTTGAGGAAAAAGAGGCTCTGGAG ACAAAGGATCCAACCAACGGTTACTACAGGGTTCGAGGGGTCAGTGTGAGCCTTAGCCTTGGGGAAGCTC CTGGAGGAGGCCTCTTCTTGCCACCGCCCTCTCCGATCGGTCTCCCAGGGACTCCTACTTACTATGACTT CAAGCCACATCTGGACTTAGTCCCTCCCTGCAGACTGTACAGAGCGAGGGCAGGTTATCTTACCACCCCC CATCCCCGTGCCTTCACCAGCTACATGAAACCCACATCCTTTGGACCCCCAGAATTGAGCTCTGGAACTC CCCCCTTCCCGTATGCTACCTTGTCTCCACCCAGCCACCAGCGTCTCCAGACTCATGTGTGAATCCATCT CTCCAAGTGAAGGGTCTTGGAATCTTCTGTTTGCCATATAGTGTGTTGTCCAGATTTCTGGGGAGTCAGA ACAAGTTGATGACCAACCCCTCCAAAACTGAACATTGAAGGAGGGAAAGATCATTACAAGCATCAGGACT GTTGGTGTACACTCAGTTCAGCCAAAGTGGATTCTCCAAGTGGGAGCAATATGGCCGCTTTCCCATGAGA AAGACATTCAAGATGGTGACTAAATGACTAAATACTTTGCAGAGGGACAAAGATGGGAACTAGGGATATG GATGGAAGTAGTAGAGAAGATATATGACCATCTGCATCAAGAGAAAGGATAACATAAGACAAATCAAGAT GAAAGAAATAATCCACACCCCCCCCCCCACCGCGTCCTGGCCAATAAGTATAGCCTACATGGCTGTTCAT TATCTGGGAACCAAAATGGCCACTATCTTGACTCCTTCCTTAAAGATACAGAAAGAATTGAATCCAAGGA ATGGGGTAGGGTGGAAATAGAAGAAATGAAGGGGACTCTTGGGCTAAGAATACTTATGTTTAATAATAAA AGGGGGAGGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6314924 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6314924 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17404 same embryo
	EMAGE:17401 same embryo
	EMAGE:17402 same embryo
	EMAGE:17400 same embryo
	EurExpress:euxassay_003831 same experiment
	MGI:4825778 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source