EMAGE mouse gene expression spatial database

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EMAGE:17427

Mms19 MMS19 (MET18 S. cerevisiae) ( MGI:1919449)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003854. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003854_01	euxassay_003854_02	euxassay_003854_03	euxassay_003854_04

euxassay_003854_05	euxassay_003854_06	euxassay_003854_07	euxassay_003854_08	euxassay_003854_09

euxassay_003854_10	euxassay_003854_11	euxassay_003854_12	euxassay_003854_13	euxassay_003854_14

euxassay_003854_15	euxassay_003854_16	euxassay_003854_17	euxassay_003854_18	euxassay_003854_19

euxassay_003854_20	euxassay_003854_21	euxassay_003854_22	euxassay_003854_23	euxassay_003854_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17427_wholemount_strong.wlz

17427_wholemount_moderate.wlz

17427_wholemount_weak.wlz

17427_wholemount_possible.wlz

17427_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17427_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7813
Entity Detected:	Mms19, MMS19 (MET18 S. cerevisiae) ( MGI:1919449)
Sequence:	sense strand is shown >T7813 GTCATGGCTGCCGCCACCGGTCTGGAGGAGGCAGTGGCGCCTATGGGCGCCCTGTGTGGCCTCGTGCAAG ACTTCGTCATGGGTCAACAGGAGGGGCCCGCTGACCAGGTGGCTGCAGATGTGAAGTCCGGTGGCTATAC AGTGTTACAAGTAGTAGAAGCCCTTGGGTCCTCTCTGGAAAATGCTGAACCCCGAACTCGGGCTCGAGGA GCCCAGCTTCTGTCACAGGTGCTGCTCCAGTGTCACTCCTTGCTCTCGGAGAAGGAAGTGGTCCACCTGA TCCTGTTCTATGAGAACCGGCTGAAGGACCACCATCTTGTGGTCCCATCTGTCTTACAGGGCCTGAGGGC ACTGAGCATGTCTGTGGCCCTGCCTCCGGGTCTGGCTGTCTCTGTGCTTAAAGCCATCTTCCAGGAGGTA CATGTACAGTCCCTGCTACAGGTGGACCGCCATACTGTCTTCAGCATCATCACTAACTTCATGCGATCAC GAGAAGAAGAGCTGAAGGGTCTGGGAGCTGACTTCACATTTGGCTTCATCCAGGTGATGGATGGGGAGAA GGATCCCCGTAATCTCCTGCTGGCTTTCCGCATCGTCCATGACCTCATCTCCAAGGACTACAGTCTGGGA CCTTTCGTGGAAGAGTTATTTGAAGTGACATCTTGTTATTTCCCTATTGATTTTACCCCTCCGCCTAACG ATCCTTATGGCATCCAGAGAGAGGATCTCATCCTGAGTCTCCGTGCTGTGCTGGCGTCTACACCGCGCTT TGCCGAGTTCTTACTGCCTCTGCTAATTGAGAAAGTGGATTCGGAGATTTTAAGTGCCAAGCTGGATTCT CTGCAAACTCTGAATGCTTGCTGTGCTGTGTATGGACAGAAGGAGCTGAAGGACTTCCTGCCCAGCCTTT GGGCTTCCATCCGTAGAGAGGTTTTCCAGACTGCTAGTGAGCGAGTAGAGGCAGAGGGCCTGGCAGCTCT CCACTCCCTGACTGCGTGTCTGTCCTGCTCTGTGCTGAGAGCTGATGCTGAGGACCTCCTTGGTTCCTTC CTCAGCAACATCCTTCAAGACTGCAGGCACCATCTATGTGAACCAGACATGAAACTTGTATGGCCTAGCG CTAAGCTGTTGCAGGCAGCTGCAGGTGCGTCTGCCCGGGCCTGTGAGCACCTCACCAGCAACGTGCTTCC CTTGCTGCTGGAACAGTTCCACAAGCACAGCCAGAGCAACCAGCGTCGGACAATCCTTGAAATGATCCTG GGATTCCTAAAGCTACAGCAGAAATGGAGCTATGAAGACAGAGATGAAAGGCCTCTGAGTAGCTTCAAAG ACCAGCTATGCTCATTGGTGTTCATGGCTCTAACAGACCCCAGCACCCAGCTTCAGCTTGTTGGCATCCG GACGCTCACAGTCTTGGGTGCCCAGCCAGGTCTCCTGTCTGCTGAGGACTTGGAGCTGGCAGTAGGTCAC CTGTACCGACTGACCTTCCTGGAGGAAGATTCCCAGAGTTGTAGGGTGGCCGCACTGGAGGCATCAGGAA CCCTGGCCACTCTGTATCCTGGAGCCTTCAGCAGACACTTGCTCCCCAAGCTTGCTGAGGAGCTGCATAA AGGGGAGTCAGATGTGGCTAGAGCAGATGGTCCCACCAAATGTTCCCGGCATTTCCGCTGTCTGCAAGCT TTGTCAGCTGTTTCAACACATCCCAGCATTGTCAAGGAGACACTGCCTCTGCTACTGCAGCATCTCTGTC AAGCAAACAAAGGGAATATGGTTACAGAGTCCAGTGAAGTTGTTGCTGTCTGTCAGAGTCTCCAGCAGGT GGCAGAAAAATGCCAGCAGGACCCTGAGAGCTACTGGTATTTCCACAAGACAGCTGTACCCTGCCTGTTT GCATTGGCTGTGCAGGCTTCAATGCCAGAGAAGGAATCCTCAGTTCTGAGAAAAGTACTGTTGGAGGATG AGGTCTTGGCTGCGTTGGCATCTGTCATTGGCACTGCCACTACCCATCTGAGCCCTGAACTAGCTGCCCA GAGCGTCACATGCATCGTGCCCCTCTTCTTAGATGGCAACACTTCCTTTCTACCTGAAAACAGCTTCCCT GACCAGTTCCAGCCGTTCCAGGATGGCTCCTCTGGGCAGAGGCGGCTGGTTGCACTACTTACGGCTTTTG TCTGCTCCCTGCCACGAAATGTGGAAATCCCTCAGCTGAACCGACTCATGCGGGAGCTTTTGAAGCAGAG CTGTGGCCACAGCTGTCCTTTCTCCTCCACTGCCGCCACTAAGTGCTTTGCAGGGCTCCTCAACAAGCAG CCTCCAGGGCAGCAGTTGGAGGAGTTCCTCCAGCTCGCTGTGGGCACAGTGGAGGCGGGCCTGGCCTCTG AATCCTCCCGTGATCAGGCTTTCACACTGCTGCTCTGGGTAACCAAGGCTCTAGTCCTCAGATACCATCC TCTCAGTGCCTGCCTGACCACCCGGCTCATGGGCCTCCTCAGTGATCCAGAACTTGGCTGTGCAGCAGCT GATGGCTTCTCTCTGCTCATGTCTGACTGCACTGATGTGTTGACTCGTGCCGGCCATGCTGATGTTCGGA TCATGTTCCGCCAGCGGTTCTTCACAGACAATGTGCCTGCTTTGGTCCAAGGTTTCCATGCCGCTCCCCA AGATGTGAAGCCAAACTATCTGAAGGGTCTGTCTCACGTACTCAACAGGCTGCCCAAGCCTGTGCTTTTA CCAGAGCTGCCCACACTTCTTTCCTTGCTGCTGGAAGCATTATCCTGCCCTGACTCTGTGGTCCAGCTTT CCACTCTGAGCTGTCTCCAACCCCTACTACTGGAAGCACCTCAAATCATGAGTCTTCATGTTGACACTCT GGTTACAAAATTCCTGAACCTCAGCTCCAGTTACTCAATGGCTGTCCGGATTGCTGCTCTGCAGTGTATG CACGCTCTCACTCGCCTCCCCACTTCTGTGCTACTGCCATACAAATCTCAGGTCATCCGAGCCTTAGCCA AGCCTCTGGACGACAAGAAGAGACTTGTGCGAAAGGAAGCAGTGTCAGCGAGGGGAGAATGGTTCCTACT TGGGAGCCCGGGGAGCTGAGCCCTTGGTCCTGGCCTAAACCCTCCTGACAATCTAACCTGCAAGTACTAA CTATTGAGCCATCCCACAAGGCAAGACTACTGGTCTGCCTTCCCATGGATGCAGTACAAATGCTGGGCCT CCTGCATGGCTGTGCAAAACTCTTAAAGAGCCATGACTTCTAATGGATTGTTGAAGAGACTGAGACTTGT ATTTGAGGAAAAGACCTTGGATGTTGGGGCTCTCCCGTGTCTGTCAGGAATAGGAAGCTGTGCTGCTGAA GGGTCAGTGCAGTGGAGTGTGACCTCGAGAACTGGGGTTGGTGTTACATGAGTTCACCTGCCAGAGAATA AAGCCTTCTTTGTAATGGGCACACTCACGTGTACTGCCTTATTCTACAGATGCTAGAGAAGCACACACCG AGAGCTCCCAGTTCTTCAGTACAGCACCGAGGCACAGAGAAGCTACATATCTTTTTACAAATCAAATGCC TTTATTTTCGCTCCCTCTGTAGCACGTGAGAAATGGCAGTGACCTTGGCAATAGGCTTACTGTCTTTGTC CCGTTGAGAAGCCAGAGTTTCAACATACCTGATGGATCTTTTCCCAAAGTGAAAGAAAGAGATGGTGTCT AGCCCCAGTGCTGTGACCTAGAGCTCGGCTGGAGCTAAAACTTATTTGCCTGAGTAAAGGGACTTCAATC CTTGAGTTTCTCCACACTGCATCTTGTTTTTAGGTGGCAGAAGATTGGTCTTGATTGTCCCTGTGTAGTA CTCTAAGGCAAGCCTGATGGCTCTTCATGGTCATGGATTCCAGGAAAAGCTGCCCTTGGAAGCTCTTGAG AGACAATAAACATCAAATAGTGTTCAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6332214 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6332214 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17424 same embryo
	EMAGE:17423 same embryo
	EMAGE:17422 same embryo
	EMAGE:17426 same embryo
	EMAGE:17425 same embryo
	EurExpress:euxassay_003854 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source