EMAGE mouse gene expression spatial database

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EMAGE:17541

Trub1 TruB pseudouridine (psi) synthase homolog 1 (E. coli) ( MGI:1919383)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005906. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005906_01	euxassay_005906_02	euxassay_005906_03	euxassay_005906_04

euxassay_005906_05	euxassay_005906_06	euxassay_005906_07	euxassay_005906_08	euxassay_005906_09

euxassay_005906_10	euxassay_005906_11	euxassay_005906_12	euxassay_005906_13	euxassay_005906_14

euxassay_005906_15	euxassay_005906_16	euxassay_005906_17	euxassay_005906_18	euxassay_005906_19

euxassay_005906_20	euxassay_005906_21	euxassay_005906_22	euxassay_005906_23	euxassay_005906_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17541_wholemount_strong.wlz

17541_wholemount_moderate.wlz

17541_wholemount_weak.wlz

17541_wholemount_possible.wlz

17541_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17541_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pectoral girdle and thoracic body wall musculature

moderate

regional

moderate expression: see section 01 02 03 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10017
Entity Detected:	Trub1, TruB pseudouridine (psi) synthase homolog 1 (E. coli) ( MGI:1919383)
Sequence:	sense strand is shown >T10017 GTGAAAGCATGGCAGCTGCAGAGGCAGAGGTGGTTTCACCTCTGATTGTGGACACAGCTCCTGACACTTC AGGAACAGCCGAGGCCTCTGTAGCAGCATCGGTGGCGGAAGCGGCCAGGACTGAATCGCAAGCCCCGGCC TCCAAGGCCGCCCTGGCTGCTAAGCTCATGTCGCTGAGCGGCGTGTTCGCCGTGCACAAGCCCAAAGGGC CCACTTCAGCCGAGCTGCCGAACCGGCTGAAGGAGAAGCTGCTGGCAGAAGCTGGAATGCCTTCTCCAGA ATGGAACAAGAGGCAAAAGCAGACATTGAAAGTCGGACACGGAGGGACGCTGGACAGTGCAGCGCAAGGT GTTCTGGTGGTTGGGATTGGAAGAGGAACAAAAATGTTGACCAGTATGTTGTCGGGGTCCAAGAGGTATA TCACCATCGGAGAACTGGGGAAAGCCACGGACACCCTGGATTCTACTGGGAAGGTAACGGAAGAGAAGCC GTATGATAAAATAACACGAGAAGATATCGAAGGCATTCTGCAGAAGTTTACTGGGAATATAATGCAAGTA CCCCCGCTCTATTCTGCGCTGAAGAAGGATGGGCAGAGACTCTCAACGCTAATGAAGAAAGGCAAGGTGG TGGAGGCCAGACCGGCCCGGCCCGTGACTGTCCACAGTATCTCCCTCCTGAAGTTCCAGCCTCCGTTCTT CACGCTGGATGTTGAATGCGGAGGAGGCTTTTACATCCGGAGCCTGGTCAGTGACATCGGCAAAGAGCTC TCTTCCTGTGCCAGCGTGCTGGAGCTGACCCGCACCAAACAGGGGCCCTTCACACTTGCACAGCACGCCC TCCCCGAAGACAGGTGGACCATCGATGACATCGAACAGTCCCTCGAGCGCTGCACGTCTCTTCTGCCGGA GGAGCTGACATTTAAAAAACTAAAATCTGACAACTCCAGTGACCAGGTTATAAGCTGTGGATATATAACT CTCAGGGACACAAAGAGAGAAGATGACGCAGTTAAGACACTGTGAGGCCGGCCTGGGAGGATCATTTCCT GGTTGGTGTCTGTCCCGACCAGGAATGCCCATACACCTCTTGAGTTCTTTGCAAGATGTCTTAATGTCTC TGAATGATGGATCTGATTGGGTTCAGATAAATAAGTTTTCTGAATTTGATCTTCCCTTAGTCTTTTTCCT ATGAAAAAGTTAAACAATATTCTAATGACAGAATCCTGAAGTCTGAGCTGCAGGTTCCACTGGTCTCACA GAAGTGTCAAACTTGCTTTGCTTAGACTCGGGGAGGGGGCGTTCGTTGTTTTGAAATTCTCTGAATACAT TTGAAGTGTTCAGTGTCGAGCTGTCCTGTGTGTTTGCAGAGCCTATGGCTGCTGGAAGGTGCTGGTGGGC TGTACTCAGCTGTGACTGTGTTTCTTAAATCCCGGGAATCAATACGTTGTCCTAATACATATCTGTAAAT GCACAAACTCCTGCTTTGGCCACTGACAGAATGTAAATAGCGTGAGACGTGATTCATTTCAGATCCTGTA TTCAGCAGAGCTCAGCTCCTGTAGCCGTGGACTGTGGCAGCTATTTCTGTGGTCAACCACATGGTATCAA AGTACCAAAAATGTGATTTTCTAGAATATAAACTAAAGAATGTATCTTGAAATAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6757213 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6757213 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17542 same embryo
	EMAGE:17546 same embryo
	EMAGE:17545 same embryo
	EMAGE:17544 same embryo
	EMAGE:17543 same embryo
	EurExpress:euxassay_005906 same experiment
	MGI:4828946 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source