EMAGE mouse gene expression spatial database

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EMAGE:17546

Nppa natriuretic peptide type A ( MGI:97367)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005873. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005873_01	euxassay_005873_02	euxassay_005873_03	euxassay_005873_04

euxassay_005873_05	euxassay_005873_06	euxassay_005873_07	euxassay_005873_08	euxassay_005873_09

euxassay_005873_10	euxassay_005873_11	euxassay_005873_12	euxassay_005873_13	euxassay_005873_14

euxassay_005873_15	euxassay_005873_16	euxassay_005873_17	euxassay_005873_18	euxassay_005873_19

euxassay_005873_20	euxassay_005873_21	euxassay_005873_22	euxassay_005873_23	euxassay_005873_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17546_wholemount_strong.wlz

17546_wholemount_moderate.wlz

17546_wholemount_weak.wlz

17546_wholemount_possible.wlz

17546_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17546_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart atrium

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22

heart ventricle

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10016
Entity Detected:	Nppa, natriuretic peptide type A ( MGI:97367)
Sequence:	sense strand is shown >T10016 AGTGACGGACAAAAGCTGAGAGAGAGAGAGAAAGAAACCAGAGTGGGCAGAGACAGCAAACATCAGATCG TGCCCCGACCCACGCCAGCATGGGCTCCTTCTCCATCACCCTGGGCTTCTTCCTCGTCTTGGCCTTTTGG CTTCCAGGCCATATTGGAGCAAATCCTGTGTACAGTGCGGTGTCCAACACAGATCTGATGGATTTCAAGA ACCTGCTAGACCACCTGGAGGAGAAGATGCCGGTAGAAGATGAGGTCATGCCCCCGCAGGCCCTGAGTGA GCAGACTGAGGAAGCAGGGGCCGCACTTAGCTCCCTCCCCGAGGTGCCTCCCTGGACTGGGGAGGTCAAC CCACCTCTGAGAGACGGCAGTGCTCTAGGGCGCAGCCCCTGGGACCCCTCCGATAGATCTGCCCTCTTGA AAAGCAAACTGAGGGCTCTGCTCGCTGGCCCTCGGAGCCTACGAAGATCCAGCTGCTTCGGGGGTAGGAT TGACAGGATTGGAGCCCAGAGTGGACTAGGCTGCAACAGCTTCCGGTACCGAAGATAACAGCCAAGGAGG AAAAGGCAGTCGATTCTGCTTGAGCAGATCGCAAAAGATCCTAAGCCCTTGTGGTGTGTCACGCAGCTTG GTCACATTGCCACTGTGGCGTGGTGAACACCCTCCTGGAGCTGCGGCTTCCTGCCTTCATCTATCACGAT CGATGTTAAATGTAGATGAGTGGTCTAGTGGGGTCTTGCCTCTCCCACTCTGCATATTAAGGTAGATCCT CACCCTTTTCAGAAAGCAGTTGGAAAAAAAAAAAAAGAATAAACTTCAGCACCAAGGACGAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:6755676 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6755676 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17542 same embryo
	EMAGE:17545 same embryo
	EMAGE:17544 same embryo
	EMAGE:17543 same embryo
	EMAGE:17541 same embryo
	EurExpress:euxassay_005873 same experiment
	MGI:4826767 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source