EMAGE mouse gene expression spatial database

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EMAGE:17676

Fstl5 follistatin-like 5 ( MGI:2442179)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006010. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006010_01	euxassay_006010_02	euxassay_006010_03	euxassay_006010_04

euxassay_006010_05	euxassay_006010_06	euxassay_006010_07	euxassay_006010_08	euxassay_006010_09

euxassay_006010_10	euxassay_006010_11	euxassay_006010_12	euxassay_006010_13	euxassay_006010_14

euxassay_006010_15	euxassay_006010_16	euxassay_006010_17	euxassay_006010_18	euxassay_006010_19

euxassay_006010_20	euxassay_006010_21	euxassay_006010_22	euxassay_006010_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17676_wholemount_strong.wlz

17676_wholemount_moderate.wlz

17676_wholemount_weak.wlz

17676_wholemount_possible.wlz

17676_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17676_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9540
Entity Detected:	Fstl5, follistatin-like 5 ( MGI:2442179)
Sequence:	sense strand is shown >T9540 GGAGCTGGTGCTGTCCACAGAGTGTCGGTGCTGAACCCCGGGTGTAAGGGATCGCCTGCTTGGGCACCTG GAGGCAACGCCCACTGGAACCCTATGTGCCCAGTGGGACCCGCTTCACCACAGGACCCAATTTAACTTGA CAAAGGAGTGCGACTCGGACGTGGGAGAGACTCCTATGTTTGCAATCTGGGCGCCCACTCAGGCGTGACT TTGGAGATTCTGTAGTTCTAACTCAAAGTATTTGGATTTTTTTTCCAACCTAAGAGGATCTTTTCATCGT TATTATTTTTCTTTTTTCTTTTTTACACTGTGACTCATATTTTCACATCATTTAGAAATCTTTTTGGAGG ACTTTTTTGTTTTTTACTTCAAATAAGGTGAAAATCAATAAAGATGTTTAGGTGCTGGTCTGCCATTTTG ATTCTTGGATTCATCTTTCTGGCATCTGAAGGGAGGCCAACAAAAGAATCAGGATATGGCCTTAAATCCT ATCAACCTCTTACAAGATTGCGACATAAGCAGGAAAAAAGTCAAGAAAGTTCAAGAATCAAAGAATTTCT AATTCATGATGGACCATTTGGATCTTGTGAAAATAAGTACTGTGGCTTGGGGAGGCACTGTGTTATCAAC AGAGAGACAAGGCATGCGGAGTGTGCCTGCATGGACCTTTGTAAACAGCACTACAAACCTGTGTGTGGAT CTGATGGGGAATTCTATGAAAACCACTGTGAAGTGCACAGAGCTGCCTGTCTTAAAAAGCAAAAGATCAC CATCGTTCACAATGAAGACTGCTTCTTTGAAGGGGACAACTGCATGGCCATTGAATACAGCAAGATGAAA AGTATGCTTTTAGACTTACAAAATCAAAAGTATATTACACAAGAAAATGAGAATCCCAACAGTGATGACA TATCTAGGAAGAAGCCGTTGGTGGATCAAATGTTCAAGTATTTTGATGCAGACAGTAATGGACTTGTAGA TATTAATGAACTAACTCAGGTGATTAAACAGGAGGAATTGAACAAGGACCTTTCTGACTGTACTTTGTAT GATCTGCTCAAGTATGATGACTTCAACGCTGACAAGCACCTGGCTCTTGAAGAATTCTACAGAGCATTTC AGGTGATCCAGTTGAGTCTGCCTGAAGATCAGAGAGTAAGCATTACCGCAGCCACTGTGGGGCAGAGTGC TGTTCTGAGCTGTGCCATTGTGGGAACGCTGAGACCCCCTATCATATGGAAGAGGAACAACATTGTTCTA AATAATTTAGATTTGGAAGACATCAATGACTTTGGTGATGATGGGTCCCTGTATATTACCAAGGTTACCA CAGTTCACATGGGAAATTATACATGCTATGCAGATGGCTATGAAAATGTCCGTCAGACACACATCTTCCA AGTGAATGTTCCTCCAGTTATCCGAGTGTATCCAGAGAGTCAGGCCAGAGAACCTGGTGTAACTGCAAGC CTTAGGTGCCATGCCGAGGGCATTCCAGACCCTCAGCTTGGCTGGCTGAAGAATGGGATTGACATTACAC CGAAGCTCTCCAAACAGCTCACACTTCAAGCAAATGGCAGTGAGGTCCACATAAGCAATGTCCGTTATGA AGATACAGGAGCATACACGTGCATTGCTAGGAATGAAGCCGGTGTGGATGAAGACATCTCTTCTCTTTTT GTGGAAGATTCTGCTAGGAAGACACTAGCTAACATATTATGGAGAGAAGAAGGTCTGGGAATTGGGAACA TGTTCTATGTTTTTTATGAAGATGGAATCAAAGTGATACAACCCACGGAATGTGAATTTCAGAGGCACAT TAAGCCCAGTGAAAAGCTCCTTGGATATCAGGATGAAGTCTGTCCCAAAGCAGAAGAGGATGAAGTGCAG AGATGTGTGTGGGCATCTGCTGTTAATGTCAAAGACAAGTTCATTTATGTCGCTCAGCCAACTTTGGACA GAATCTTGATTGTTGATGTGCAATCCCAAAAGGTTGTTCAGGCAGTGAGTACAGATCCTGTACCAGTTAA ATTACACTACGACAAGTCACATGACCAAGTCTGGGTGCTGAGCTGGGGCAGCATGGAGAAGACATCACCA ACTCTACAGGTGATCACTCTGGCCAGCGGGAATGTGCCACACCACACCATCCACACTCAGCCAGTTGGGA AACAGTTTGACAGAGTGGACGATTTTTTTATTCCAACTCCAACACTTATTATTACTCACATGAGATTTGG GTTTATTCTTCATAAAGATGAATCTGCACTACACAAAATTGATCTCGAAACCATGTCCTACATCAAAACA ATTAACCTGAAGGACTCACGATGCATTCCTCTATCCTTGGCATACACCCATCTGGGAGGATACTACTTCA TAAGCTGCAAGGCTGACATCACCGGAGCTACTCCACCACAGCTCGTGGTAGACAGTGTGACTGACTCTAT CATTGGCTTCAATAGCGATGTGACGGGCACTCCTTATGTCTCTCCAGACGGACACTATCTTGTCAGTGTT AATGACGTGAAAGGTCTTGTCAGAGTCCAATATATCACCATCCGAGGAGAGATTCTGGATGCTTTTGACA TCCACACCAACCTACATATATCTGACCTGGCCTTCCAGCCATCCTTCACAGAAGCCCACCAGTACAACAT CTATGGCAGCTCAAGCCAACAAACAGATGTACTCTTTGTGGAGCTTTCCTCTGGGAAGGTCAAGATGATC AAAAGCCTCAAAGAACCTCTCAAGACAGAAGACTGGCCCTGGAGCCAGAAAAACAGGCACATCCAAGGCA GTGGCCTGTTTGGACAGTACCTGATGACACCTTCCAAAGATTCTCTCTTCATCCTGGATGGTCGCCTTAA TAAATTAAACTGTGAGATCACTGAGGTTCAGAAAGGAAATACAGTTGTTTGGGTTGGCGATGCCTAAGGA GCCCTCTTACCTAATTATGAAAGAAGAGTTTTACAGTACATTGCACTTCATCCATATTTAAATTACAACT TAACTTTTCCAAATTTATATTCTAGTCAAATCCCCTCTGTTTGTTCAAATAAGATAGATTTTTTTTTACA ATGATAATTGGCTAGTTAGAAACATTTCATACAATATTTAATTGGAAATTACAGCACTCACAAGAATCAA ACATGTAGCTATATTTTAAACCAATGAAATGAGATTTAAATTATTTAAAAAAAAAATACACTGCAGGGAA TTTCAGATGACCTAAGCATCATGTCATTCTTTACGGCATTTTTTTTTCCTCTGTTATTTTGCTATGCTTA AATCAGAAATAAAAGGTCGTGTTAAACCTTAAAATTCTTAATTCAAAATACTCTGTCCCGTCCACTGTTT AAAATCTTTTGTGCCTTGAAGGAAATGTCATGGAAGGAAAAAAAAAAAAGGAAGGCTAAGCTCTGCATAT TGGACAACCTCATAAGCAATGAATCTACTGTCATTAAGAAATGTTTATATCTTGCGCCAAATCCTGCCTT CAGGCATTTGTTTGACCTTCAATGAAATGTCATGTGAAGGCATAATTTGGCCTTGGGCTGTATACAGATG AGAAGCTATCCAACATTCACTGTACTATGTCCATTGTGCTATAGAAAATGGACAGGAAGGGAGAAAAATT TGTATGGTAAAAATGTTTGCAATGTATTGTGTCCACATTTTGCTTTCATTCACTAGAGTGCATTATATTA TTGGTGTATGATTATCTGGGAATTCAACTGCTTACAGAAGGTTTCCTTCTTTAGTATCAAGATTGATTCT TTTGTGTAGCCTAATGTTACATAAGTCAGGAGCTTTCTCAGTTCTGAAAAAGGAGCATGACTGTGTCTGC ACAGAAAGAACCACTTCTAGGGAAATTGACAGTGAGACAAAGCTGAAACCACTCTCTGTAGTGCGGTGGC CTATATCCTCTTCAGACAACATTCACTTGGTTGGTCCAAAGAAGGTGAATGGGTTTTAGCAGAAAACAGT AACATGAGCCCACAGTAGAGATTGGGAAGTGAACTCCACTGCTTTTGCGATTTAGATGTATCTATTAAAT AAATCCATCCTTTATTAATGGTCAAGATGACTTCTACTCCTGCATATTCTAAACCACCCTCTCTTTAACT GTGTGCTCTAGCTGTAACCCAACGGAGTCTCTTGAGATATAGTAGAGAAGTAAAAGTGACTGCCTTTATC CTTTTTCCTACAGGAGAAAACAGAAAAACAGTAGAAATAGATTAATACCAATAGTTTGGTTATTGGAATA AAAGAAAACAATCCAGGTGTAAGGGAATAGCATACATGGTTTGAAAATCTTTTCTACTTATTTTTCTTCT TATTGTTGCTAAAACTCGTGATAAGCTATTGAACTAATGCCCATTTGCTGAAGGTAATCCAGTAAGATCT TTTTTTAAATACAAATTGGGTAAAACTGCATAGAAACTTGGGATTTTATAGTGTGCACTCTCTTTTGTGT ATATAGCACCATGGGCTGTGTGATAATAGATTTCTTCTTAGCTTAAGGATACATTAAGGGAAATAGATTT AATAAACAACAGAAATCAGCCTACATTTTTACTGATTGAGTCTGTTTTACTCTTTAGTAACTTCGTCCCC TGGGAACATGAATCATGCAGTGTCTTTGTGTTAATGATTTCGTGTAAGTCTATCCTGTACACGTACATCT TCTTTTAGAAGCTTTTACTATGTGGATGCTGTCTGTTATCATTGCTTGATAAAAGAATAAACCTCTTATG TTTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30545529 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30545529 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17680 same embryo
	EMAGE:17677 same embryo
	EMAGE:17679 same embryo
	EMAGE:17678 same embryo
	EMAGE:17681 same embryo
	EurExpress:euxassay_006010 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source