EMAGE mouse gene expression spatial database

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EMAGE:17678

Swap70 SWA-70 protein ( MGI:1298390)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006014. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006014_01	euxassay_006014_02	euxassay_006014_03	euxassay_006014_04

euxassay_006014_05	euxassay_006014_06	euxassay_006014_07	euxassay_006014_08	euxassay_006014_09

euxassay_006014_10	euxassay_006014_11	euxassay_006014_12	euxassay_006014_13	euxassay_006014_14

euxassay_006014_15	euxassay_006014_16	euxassay_006014_17	euxassay_006014_18	euxassay_006014_19

euxassay_006014_20	euxassay_006014_21	euxassay_006014_22	euxassay_006014_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17678_wholemount_strong.wlz

17678_wholemount_moderate.wlz

17678_wholemount_weak.wlz

17678_wholemount_possible.wlz

17678_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17678_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9546
Entity Detected:	Swap70, SWA-70 protein ( MGI:1298390)
Sequence:	sense strand is shown >T9546 GAGTTGCCGACTGGGCTGACCAGGTCTGTTAGGGCAGCGCGGCCGCGGCCATGAGGGGCTTGAAAGACGA ACTGCTCAAAGCCATTTGGCACGCCTTCACCGCGCTCGACCTGGACCGCAGCGGCAAGGTCTCCAAGTCG CAACTCAAGGTCCTTTCCCATAACCTGTGCACGGTGCTGAAGGTTCCACATGACCCGGTTGCCCTTGAGG AGCACTTTAGGGATGACGATGAGGGGCCTGTCTCCAATCAGGGCTACATGCCATATTTAAACAAGTTCAT TTTGGAAAAGGTCCAAGACAACTTTGACAAGATTGAATTCAATAGAATGTGTTGGACACTTTGTGTCAAG AAAAACCTCACAAAGAGTCCTCTACTCATTACAGAAGATGATGCATTTAAAGTGTGGGTCATTTTCAACT TTTTGTCAGAGGACAAGTATCCACTAATTATTGTGCCAGAAGAGATTGAATACCTGCTTAAGAAGCTTAC AGAAGCTATGGGAGGAGGTTGGCAACAAGAACAATTTGAACATTACAAAATAAACTTTGATGACAATAAA GATGGCCTTTCTGCATGGGAACTTATTGAGCTAATTGGGAATGGACAGTTTAGCAAGGGCATGGACCGTC AGACCGTATCTATGGCCATTAACGAAGTCTTCAATGAGCTTATTTTAGATGTATTGAAGCAGGGTTACAT GATGAAGAAAGGTCACAAACGGAAAAACTGGACTGAGCGCTGGTTTGTATTAAAACCCAACATAATTTCC TACTATGTGAGCGAGGATCTGAAAGATAAGAAAGGAGACATCCTGCTGGATGAAAACTGCTGTGTGGAGT CTCTGCCTGACAAAGATGGAAAGAAATGTCTTTTTCTAATAAAATGCTTTGATAAGACCTTTGAAATCAG TGCCTCAGATAAGAAGAAGAAACAAGAATGGATTCAGGCCATTTACTCCACCATCCATCTGTTGAAGCTG GGCAGCCCCCCACCACACAAGGAAGCCCGCCAGCGTCGGAAAGAGCTCCGAAGGAAGCTGCTAGCCGAGC AGGAGGAGCTGGAGCGGCAGATGAAGGAACTCCAAGCCGCCAATGAAAACAAGCAACAGGAGCTGGAAAG CGTGAGGAAGAAACTGGAGGAAGCAGCCTCTCGTGCGGCAGACGAGGAAAAGAAACGCTTGCAGACTCAG GTGGAGCTACAGACCAGGTTCAGCACGGAGCTGGAGCGGGAGAAGCTGATCAGACAGCAGATGGAGGAGC AGGTTGCCCAGAAGTCCTCCGAACTGGAGCAGTATCTGCAGCGAGTTCGGGAGCTGGAAGACATGTACCT AAAGCTGCAGGAGGCTCTTGAGGACGAGAGGCAGGCCCGGCAGGATGAAGAGACTGTGCGCAAGCTTCAG GCCAGGTTGCTGGAGGAAGAGTCTTCTAAGAGGGCAGAGCTGGAAAAGTGGCACCTGGAGCAGCAGCAGG CCATTCAGACAACAGAGGCGGAGAAGCAGGAGCTGGAACAGCAGCGTGTCATGAAGGAGCAGGCATTGCA GGAGGCCATGGCACAGCTGGAACAGTTGGAGTTGGAGCGGAAGCAGGCCCTGGAGCAGTATGAGGGAGTT AAAAAGAAGCTAGAGATGGCAACACATATGACCAAGAGCTGGAAGGACAAAGTGGCCCATCATGAGAGAT TAATACGATTGATAGAACCAGGTTCCAAGAACCCTCATCTGATCACCAACTGGGGACCCGCAGCGTTCAC CCAGGCAGAGCTCGAGGAGAGAGAGAAGAGCTGGAAAGAGAAGAAGACCACAGAGTGACGGCCGTCCCAG CGGTCACCCAGTCAGTCACATCAGTGAGGAGTCAAGAGCTTCATACCTGGGACAAGAACATTGGCTTTGC TGCTTCTAAGTCTCCCCTCAGGGCTCCAGCAGGGTCCTGTGCTGAGGACGCCATGTTTACCTTCTAGAGA TGTCTCAGCTCTCCTGGCGAGAACATAGGCACAGGTGTGGCTGGAGCCACCTCACTGTTGTCTTCTGCAG CCCTTGCAGCCCCTGGTCTCAGCCCCGCCTTGGATGGGAGGTCAGCTGTCTCCTCCCCTCCGGCAGCGCA TTCCCTTGGCCGATTGCCTGGTCTGTTGGCCTCTTTGAGTCAGGTGTCATGGCAAGCCTCCGCTGTTGGT GCTCTGATGGACAAGTCACTAGTCACTGCCCTTGTGGATGGAATCTGGTTGGTGACTCTTCCCAGCTTCA GCTAAGATACTTCCCTCCCTGACCACCACACACATGGCATGCTTCGTTTGCATTTTCTTTCTCCTCACCT CAAAGCAAGACTTATCCTGGCCCAGCGGCTCAACTTAGTTCTTTGGGTGTTCTGTCCCCAGCTGCTAGTC TGTTGAAATCAATGGTGATTGGATAGTTTGGGGGGTTACATGGGCACAGTGGAAGCTGACAGAATGTGGA CTGGTAAGATGTAAAGTGTCACAGGATGATGGCCAGCCTTGTGGCTTTCACTAGAACTATGTCTCTCTGC TTTTCCCTCCGAGGATGACACAGATTGTGGTTTGTATGCTTCTTGTTTGAGGAATGATTTCAGCCCTAAT CTGGTCTGAGACGGAGATGAGTGGTTGGAAAGTAAGCCTTACCACACTAGTTGGAATCTGTAACCAGCAG TAACTCCATGCACAGCCAAGAAGCTTTACATTATAAACAGGAAGGTGACCCATGTGGCACAGAAGAGGTG GGACTCAGTAGAGAGCAGGGACCCCCACGGGCCTGGTCCAGCCTTGCCTCAGAGCAGGAAGGAGGCCATT CCAGACATGTGCAAGTCTACAGATAGATCCCAGCAGTCCTGGCATCTACATAAGGACTGGATAGAGTTCC ATTAATCTCTGCCTTTTATGACTGGAAAACATTTGCCAATGGAAGTCTTACCAATTTTTCTTAAGATTTT GGGCTGCTTAATAAAAGTAATTTTATTTAATGTTTATAGTTGCCTTCATACAGTTTAGATTTGTGTGCAA AGTATCACTGTGTCACGAACCTTCTTAGTGGATAACTTGGGTGTAGGTTTGGACCTGGCCATTCACTCAC CATGTTTCCTTGTCAGTGCAGCCGGGAATCAGACCAAGGGGACCAGGGTCTCCCCTGTGTCTTGATGATG TCTCCTAGAGACCTCAGTGTCCTTGGAGAGAAAAGAAAAAGAAACTCCAAGAAGGTACTGGCCGGTGTGA AATGTCTGGACGGAAGCAAGGGCCTGGGGGTCCAGGGGAGACTGGGGTAAAGGGATCGCCCGGGCCGGGC TGACAGAACCTCCCTCCTTAAACCTGGACTGTCTTACAGCACAGCCAGAGGCATGGCCCGCAGCTGCCAC GGCCCTCCTTGTCTGGTCTCAAGCCTGAATACTGCATGCTCCCTTTGTATGGGCTGTGCAGAAGGCGTCT GCAGTCTGTAGTGTTTGTTTCTGCTTGCCTCACCGGCTTCAGTAGCACTGTGGCCGGGAAGTGAAAGTCC TTTGTAGACAGTGGATGAGAAGACCGCACAGATGTGAACTGTGTGACTCCCCTTTAGGCCAATGCGCTGA TACTTGTGGGTCTCTCCTGATACTCAAGGTGGTTTCAGCTAAGTGTCTATAGACCGTGCCTCATCTCTGT AATAACTACAGGAAACGTTGGGAGTGTCTGTCTGTGTGTTCACTGTAATCAGCCCTTTCCTCCAACGAGA GATGAGATTATTTGCAGGAATTATGAATGAAAAGCATTTGAAAATGTTTTGGGATCTACTATATTGAGAA TGAACAAAATATTTATAATTTTAAAACATTTTTATCTACACATACAATGTAATATGCTCTACTTCTTGTT TTTTTTTTGTTTTGTTTTTTTGCTAATTTTTCTAAGGTATTAAAATGTTTTCTATGGGATTTTTGGTTTC TATTTTTTTAGTAAAGTGGACTCAATAACAAAATTAAACATACCAGAATTTCATACAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30546814 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30546814 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17680 same embryo
	EMAGE:17677 same embryo
	EMAGE:17676 same embryo
	EMAGE:17679 same embryo
	EMAGE:17681 same embryo
	EurExpress:euxassay_006014 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source