EMAGE mouse gene expression spatial database

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EMAGE:17680

Prkaa2 protein kinase, AMP-activated, alpha 2 catalytic subunit ( MGI:1336173)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006001. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006001_01	euxassay_006001_02	euxassay_006001_03	euxassay_006001_04

euxassay_006001_05	euxassay_006001_06	euxassay_006001_07	euxassay_006001_08	euxassay_006001_09

euxassay_006001_10	euxassay_006001_11	euxassay_006001_12	euxassay_006001_13	euxassay_006001_14

euxassay_006001_15	euxassay_006001_16	euxassay_006001_17	euxassay_006001_18	euxassay_006001_19

euxassay_006001_20	euxassay_006001_21	euxassay_006001_22	euxassay_006001_23	euxassay_006001_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17680_wholemount_strong.wlz

17680_wholemount_moderate.wlz

17680_wholemount_weak.wlz

17680_wholemount_possible.wlz

17680_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17680_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart ventricle

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

left lung

moderate

regional

moderate expression: see section 04 05 weak expression: see section 03 06 07 08 09 10 11 12 13

right lung

moderate

regional

moderate expression: see section 22 23 24 weak expression: see section 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T218
Entity Detected:	Prkaa2, protein kinase, AMP-activated, alpha 2 catalytic subunit ( MGI:1336173)
Sequence:	sense strand is shown >T218 ATTTCCTGAAGACCCCTCCTACGATGCGAATGTCATTGACGATGAGGCTGTGAAGGAAGTCTGTGAGAAA TTCGAGTGTACAGAGTCAGAAGTGATGAATAGTCTGTATAGTGGTGACCCTCAAGACCAGCTTGCAGTGG CTTATCATCTTATCATTGACAATCGGAGAATAATGAACCAAGCCAGTGAGTTCTACCTCGCCTCTAGTCC TCCATCAGGTTCTTTTATGGATGACAGCGCCATGCATATTCCTCCAGGCTTGAAACCACATCCAGAAAGG ATGCCGCCTCTCATCGCAGACAGCCCCAAAGCACGCTGTCCACTGGATGCACTCAACACAACGAAGCCCA AGTCTCTAGCTGTGAAAAAAGCCAAGTGGCATCTTGGAATCCGAAGCCAGAGCAAAGCGTGTGACATTAT GGCTGAAGTGTACCGAGCTATGAAGCAGCTGGGTTTTGAATGGAAGGTAGTGAATGCATACCATCTTCGA GTAAGAAGAA
Notes:	The probe template was PCR amplified from IMAGE:2649062 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2649062 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17677 same embryo
	EMAGE:17676 same embryo
	EMAGE:17679 same embryo
	EMAGE:17678 same embryo
	EMAGE:17681 same embryo
	EurExpress:euxassay_006001 same experiment
	MGI:4827414 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source