EMAGE mouse gene expression spatial database

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EMAGE:17707

2410042D21Rik RIKEN cDNA 2410042D21 gene ( MGI:1919675)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006063_01	euxassay_006063_02	euxassay_006063_03	euxassay_006063_04	euxassay_006063_05

euxassay_006063_06	euxassay_006063_07	euxassay_006063_08	euxassay_006063_09	euxassay_006063_10

euxassay_006063_11	euxassay_006063_12	euxassay_006063_13	euxassay_006063_14	euxassay_006063_15

euxassay_006063_16	euxassay_006063_17	euxassay_006063_18	euxassay_006063_19	euxassay_006063_20

euxassay_006063_21	euxassay_006063_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17707_wholemount_strong.wlz

17707_wholemount_moderate.wlz

17707_wholemount_weak.wlz

17707_wholemount_possible.wlz

17707_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17707_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4122
Entity Detected:	2410042D21Rik, RIKEN cDNA 2410042D21 gene ( MGI:1919675)
Sequence:	sense strand is shown >T4122 GTGAGTGGGGCCCGGCTGGGGATCCGAGGGACGCTCCGAGCTGCCGGGAGCGGTTCATCAGCACAGTGGC TGAGCTTGGGCGCTGCGGTACCATGAGGCGCCGGCACTTAAGAGATTATGGCATTTGATACTCACCATGT TAAAAAACGGAACTTCTCTAATAGTATTGATCTTCCTAGAAAGAGGATCTCTAACTTCACCAGTAAGAAC ATGAAGGAGGTTAAGAGATCTCCAAAACAGTTGGCTGCTTACATAAGTAGAACAGTTGCACAGGCAGTGA AAAGCCCAGAAAAACTGCGCAAGGTGCTCTACCACAGAAAGTTAGTTCGTCGTTCCTTCCCAAATCCTTG TTACAAAACTAAGCAGTCCCCAAAAAGTGGGGGCTGTGACATGGCAAATAAAGAAAATGAACTGGCTTGT GCAGGCCATCTACCTGAAAATTTACGCCACGACAGTCGGACATTTGTCATTAATACCAGTGATTCTGGGT CTTCACAGACAGAAAGCCCATCATCAAAATATAGTGGTTTCTTTTCTGAAGTTTCTCAGGACCATGAGAC AATGGCCCAGGTTCTATTCAGCAGGAATTTGAGATTGAATGTAGCTTTGACTTTCTGGAGAAAGAGAAGT ATAAGTGAACTTGTAGCTTATTTAGTGAGGATAGAAGACCTTGGAGTTGTGGTAGATTGCCTTCCTGTTC TAACCAATAGTTTACAGGAAGAAAAGCAGTATATCTCACTTGGCTGCTGTGTAGACTTACTTCCTCTGGT AAAGTCTTTACTTCAAAGCAGATTTGAAGAATATGTAATTGTTGGTTTAAACTGGCTTCAAGCAGTAATA AAAAGGTGGTGGTCAGAACTCTCATCTACGTCAGAAATTATAAGTGATGGAAATATTAAGATTTTAAAGC AACAGTTGAGTGGACTATGGGAACAGGAAAGCCATCTTACTTTGGTTCCAGGATATACTGGTAATATAGC TAAGGATGTAGATGCTTATTTATTACAGCTGCATTGAAAGATTTCATCTTATAAAGATTTGAGTATTCAG ACAGCGCTGGATTCTATGATTTCCAAAAGGAGGTCTCTCCTGAGAACCGAGAGCTGTGGAAGAAATAAAA CCAATTTTTTTCTTTTAAAAAGCCACATAATAAAACCACTAATGAAATCCTAACAATCTACTTCACATTG AAGTGGAAATATCAAGAAGGAGCGGCATCAACTTCAATGAGGTGAAAGTGCACTATGAAGACTGTTTGCC TTTGCTGCATTCAGAATTCAAATGACTGTTTGATAGCGTTGTTTAAGAACTACTGGGTCTTCACTCGGCC CTGCATAAGAATATGATTGATAGTATGCAAAAATAAGACAGGACTTATTACCAGGAACTATAAAGACCAA TCATTAAATTTTTTATTGTGTTTTATGAAGAAACACTTAAGTGTGTGCAGCTAATTTCTTCTGTTGGAAA GACTTGGAAAGTTTAAAACATCATAGTAAGTAATATTAAAAATTTTTGTCCACACTGATACTGTGTACAA ATGCCTTAATTATTGATAAGCCAATATGTTATAATACCAATATCTTTTTTAAAAAAATTAAAAGCAACCA TGCTCTGGCATGATAAAATCATGGAATTAAATCAGGGGTTTACATTCATGTAGAATGTTGTTGAAATATA TTCGACACCATTTTTTTAAACTTGAGAAATTTTTAGCATCTCTGATTCTTTTTGTCAGAAACTTCATGTT ATGTTTGTATGTATGTGTTATTCTTATATAAAGAAAAAAGGTGAATATGTATTTGTATGAATGTTTAACT GGAAATGTCCATGGAGTTGGCTAATTTATATTCACTTTTTATTGTATATAGAGTTCTAATATTTTTCATT TCTGTAACATTTAAACTTCCTTTGAGTAAATTTACTAAATATTTCTATTTTTTGCTTTTTTAATTCTAAT TTTATATGAAATCTGATTAATTCCTTGACACTAAATGTGTTCTGAAAAGTTCCACACAATGTTTCAGAAT TGTTTACAGTTAATGCTGACATTGTACTTTAAGTTCCAACACAGTGCATCACTACCTCCTCTAATGGTTA GTAGGAAATTCCAGCAAACCATATGCAAGCGTTTTTGTGTTTTGTTTTGTTTTTTTTTTAATATGGCCAT TTGAAGTATCAGTGTACTCAATTCTAGGAAGTCATAACAGTTTGAATCGTACCTGTCTTAGTTGGAATTG CAGTTAGCTACCACTCCTGCTGGCCCATTCATCATCTTTCTTTAGTAAGGCTTGCAAGCTTCCAGAAAAG ATAAATTGACTTCCTCTTCATATTCCAATGTACATTTTAAAACTTAAAGCAGTGACTACTGAGACCCCCA GCCCCCCTGCCCTCCAGTGTTATTTTTTAACTGAATTCTGTTTTGTATAGCTATTATTCCAGATATCTAT GTACACCACAGAATGAATATCCGTTGTAGTAATTGATTATTATATCCTCAGATTAGAGTCACATCCAAAT GACTACAGTTTTGTGATTTCGAGGTGAGGTATGTAAGAAGTCATTCTTCAGAACGTGGGGGTGTCATGAA CCTCCATTCCCCTGAAGCCTTCAGCTGGGAACTACAGCCTTTCTTTCTTCTTAGCCTGCAGTTTTCACTG TCTTATTGAACCATTTGGGCAGGTTGATGTGGTTTGGTTGGAGTGTGATGCTAGTGAGACTAAGGGTTTG AGTCTGATCCTTTTAAATTCATTAACTTCTCACGTGCACAAAGAAATTCTGTAGTTCCTGATTGCATATT TCTTTCAGTATCCACTTAATAAATTCAGTTTGTGATCTCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4460520 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4460520 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17711 same embryo
	EMAGE:17708 same embryo
	EMAGE:17710 same embryo
	EMAGE:17706 same embryo
	EMAGE:17709 same embryo
	EurExpress:euxassay_006063 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source