EMAGE mouse gene expression spatial database

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EMAGE:18300

Btbd11 BTB (POZ) domain containing 11 ( MGI:1921257)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012089. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012089_01	euxassay_012089_02	euxassay_012089_03	euxassay_012089_04

euxassay_012089_05	euxassay_012089_06	euxassay_012089_07	euxassay_012089_08	euxassay_012089_09

euxassay_012089_10	euxassay_012089_11	euxassay_012089_12	euxassay_012089_13	euxassay_012089_14

euxassay_012089_15	euxassay_012089_16	euxassay_012089_17	euxassay_012089_18	euxassay_012089_19

euxassay_012089_20	euxassay_012089_21	euxassay_012089_22	euxassay_012089_23	euxassay_012089_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18300_wholemount_strong.wlz

18300_wholemount_moderate.wlz

18300_wholemount_weak.wlz

18300_wholemount_possible.wlz

18300_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18300_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 weak expression: see section 13 14 15

hypothalamus mantle layer

moderate

regional

moderate expression: see section 11 weak expression: see section 10 13 14

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 09 10 11 12 13

cerebral cortex mantle layer

strong

regional

strong expression: see section 20 moderate expression: see section 01 02 03 04 19 21 22 weak expression: see section 23

cerebral cortex marginal layer

strong

regional

strong expression: see section 05 06 20 moderate expression: see section 01 02 03 04 07 15 16 17 18 19 21 22 weak expression: see section 08 09 14

medulla oblongata alar plate marginal layer

strong

regional

strong expression: see section 05 06 07 08 16 17 18 moderate expression: see section 15

rest of cerebellum marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 moderate expression: see section 12

pons mantle layer

moderate

regional

moderate expression: see section 07 15 weak expression: see section 05 06 08 09 12 16 17 18

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 06 07 18

trigeminal v ganglion

strong

single cell

strong expression: see section 02 03 moderate expression: see section 04 05 06 07 16 17 18 19 20 21

dorsal root ganglion

weak

single cell

weak expression: see section 07 08 14 15 16

renal cortex

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30711
Entity Detected:	Btbd11, BTB (POZ) domain containing 11 ( MGI:1921257)
Sequence:	sense strand is shown >T30711 CGGCGTACTGTGAAGGCTACTTTCTTAAAAATATGATGGTCCTTATTGAAAACGAAGCGTTCAAGCAGCT CCTGTACGACAAAAATGGCGAAGGGGCGGGCCAGGATGTGCTTCAGGACCTACAGAGGACGTTGGCCATT AGGATTCAGTCTATCCACCTGTCGTCTTCCAAGGGTTCCGTGGTATGAGACGCCCCACGAGGCAATGGTC CCCAGGAGCAAGGTTTCTGGCTCCCTTCTGCATTGGCACCACAAAAACACAAAAATTGTTCCTGGTGTCT GTCTGTCTGTACTGGGCCAAGGAGCTGCCTCCACTGCCTCAGCTACTCTCCGGTCAGTGAACTTGGCTTC CGTGTGCTCCTGAGCGAAGAACCGTCCACGCGTCTGCAGGTTAGATTGTAGCAGAGGCCAGAACCTGCAG GCCAACTCACAGCAACCAAGCCCCCTCCAGTGCCCCGGAGCCATGGGCACCTGCTGCCAAGGACCATGAT CTAGCAGACGGACCAAGGCTTAGAGATCTCTGCTTCCTGGTTGACTGATGAAAACCTCAGTAGTATGACC TTGAAAAGCATCACATCACCCACGAATGACCTTGGAGGCTGTACTTGCCCAGGCATCAGCCCTGGTGGCT AGTTGTTGATGCCATTGCCCGGCATGAGGAAGTCACATGGCCAATTTTTAGGGGTGGGAACTTTTTAAAA AAAATGTTCATGTCTAGCAAACACAGAGCAGTCTCGTAGTTTCGAATATATTTCTAAAAGCTTAACAGTT CATTTTCGACTGAATTGTGTTTAGGGAATCTGTGTTTGGGGTTCTTTTCATTTTTTTTTAAGATAGTGAG TTGCAGGTCCGTGCCGTGTAAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6812291), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60397. Forward Primer - name:060397_F_IRAV119_d05_Btbd11, sequence:CGGCGTACTGTGAAGGCT; Reverse Primer - name:060397_R_SP6_IRAV119_d05_Btbd11, sequence:GTTTACACGGCACGGACC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18303 same embryo
	EMAGE:18299 same embryo
	EMAGE:18302 same embryo
	EMAGE:18301 same embryo
	EMAGE:18304 same embryo
	EurExpress:euxassay_012089 same experiment
	MGI:4823513 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source