EMAGE mouse gene expression spatial database

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EMAGE:18304

Parp6 poly (ADP-ribose) polymerase family, member 6 ( MGI:1914537)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012100. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012100_01	euxassay_012100_02	euxassay_012100_03	euxassay_012100_04

euxassay_012100_05	euxassay_012100_06	euxassay_012100_07	euxassay_012100_08	euxassay_012100_09

euxassay_012100_10	euxassay_012100_11	euxassay_012100_12	euxassay_012100_13	euxassay_012100_14

euxassay_012100_15	euxassay_012100_16	euxassay_012100_17	euxassay_012100_18	euxassay_012100_19

euxassay_012100_20	euxassay_012100_21	euxassay_012100_22	euxassay_012100_23	euxassay_012100_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18304_wholemount_strong.wlz

18304_wholemount_moderate.wlz

18304_wholemount_weak.wlz

18304_wholemount_possible.wlz

18304_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18304_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

strong

regional

strong expression: see section 02 03 04 05 17 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 17

trigeminal v ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 07 15 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 07 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 15 16 17

trigeminal v nerve

strong

regional

strong expression: see section 07 15

spinal cord

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 15

cervical ganglion

strong

regional

strong expression: see section 07 moderate expression: see section 08 15 16

thoracic ganglion

moderate

regional

moderate expression: see section 11 12 13

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

neural retina

strong

regional

strong expression: see section 01 20 21 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 05 06 07 08 09 10 12 13 14 moderate expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30717
Entity Detected:	Parp6, poly (ADP-ribose) polymerase family, member 6 ( MGI:1914537)
Sequence:	sense strand is shown >T30717 GGAGCAGCCTATGGCAAAGGCATCTACCTGAGCCCCATCTCCAGTATTTCCTTTGGATACTCAGGAATGG GCAAAGGACAGCACAGGATGCCTTCCAAGGATGAGCTGGTCCAGAGATACAACAGGATGAACACCATCCC CCAGACCCGATCCATTCAGTCAAGGTTCCTGCAGAGTCGGAATCTAAACTGTATAGCACTTTGTGAAGTG ATTACGTCTAAGGACCTCCAGAAGCATGGGAACATTTGGGTGTGCCCTGTGTCTGACCATGTCTGCACAA GGTTCTTCTTTGTATATGAGGATGGGCAGGTGGGCGATGCCAACATTAATACTCAGGACCCCAAGATTCA GAAGGAAATCATGCGTGTGATCGGAACTCAGGTTTACACAAACTGAGGGGGCCCCAGCCCTCGTACCACC CTGTTACCCCAGGATCCATCTGCCCTTGTAAAAGGACTCAGGAGCAGCAAGTGAGGCTGTCCTGAGAAAC TCAAGGGGCAAAAAAAGTATAGAAGAGGTGGCCTTCATGGTGGAGATTGATCCAGGAACTACTCCACATT TGGATGGCCCTGACTGACTCCCACCCCTGATTTGCCCAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5716648), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60359. Forward Primer - name:060359_F_IRAV119_g02_3110038K10Rik, sequence:GGAGCAGCCTATGGCAAA; Reverse Primer - name:060359_R_SP6_IRAV119_g02_3110038K10Rik, sequence:ATTGGGCAAATCAGGGGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18300 same embryo
	EMAGE:18303 same embryo
	EMAGE:18299 same embryo
	EMAGE:18302 same embryo
	EMAGE:18301 same embryo
	EurExpress:euxassay_012100 same experiment
	MGI:4827053 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source