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EMAGE:18409

Cebpz CCAAT/enhancer binding protein zeta ( MGI:109386)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18409 EMAGE:18409 EMAGE:18409 EMAGE:18409 EMAGE:18409
"Pseudo-wholemount" of euxassay_014370. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014370_01 euxassay_014370_02 euxassay_014370_03 euxassay_014370_04
EMAGE:18409 EMAGE:18409 EMAGE:18409 EMAGE:18409 EMAGE:18409
euxassay_014370_05 euxassay_014370_06 euxassay_014370_07 euxassay_014370_08 euxassay_014370_09
EMAGE:18409 EMAGE:18409 EMAGE:18409 EMAGE:18409 EMAGE:18409
euxassay_014370_10 euxassay_014370_11 euxassay_014370_12 euxassay_014370_13 euxassay_014370_14
EMAGE:18409 EMAGE:18409 EMAGE:18409 EMAGE:18409 EMAGE:18409
euxassay_014370_15 euxassay_014370_16 euxassay_014370_17 euxassay_014370_18 euxassay_014370_19
EMAGE:18409 EMAGE:18409 EMAGE:18409 EMAGE:18409 EMAGE:18409
euxassay_014370_20 euxassay_014370_21 euxassay_014370_22 euxassay_014370_23 euxassay_014370_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18409Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18409_wholemount_strong.wlz
18409_wholemount_moderate.wlz
18409_wholemount_weak.wlz
18409_wholemount_possible.wlz
18409_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18409_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 moderate expression: see section 11 15 17 18 19 20 21 22 23
radius
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 24
ulna
strong strong
regionalstrong expression: see section 02 moderate expression: see section 24
humerus
strong strong
regionalstrong expression: see section 01 02 03 04 05 moderate expression: see section 21 23 24 weak expression: see section 20
hindlimb digit 2 phalanx
strong strong
regionalstrong expression: see section 11
hindlimb digit 3 metatarsal
strong strong
regionalstrong expression: see section 07
hindlimb digit 3 phalanx
strong strong
regionalstrong expression: see section 07 11
hindlimb digit 4 metatarsal
strong strong
regionalstrong expression: see section 07
hindlimb digit 4 phalanx
strong strong
regionalstrong expression: see section 07 11
hindlimb digit 5 phalanx
strong strong
regionalstrong expression: see section 11
fibula
strong strong
regionalstrong expression: see section 01 02 03 04
tibia
strong strong
regionalstrong expression: see section 01 02 03 04 05 06
femur
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 moderate expression: see section 17 18 19 20 21 22 23
otic capsule
strong strong
regionalstrong expression: see section 07 08 09 moderate expression: see section 15 16 17
viscerocranium
strong strong
regionalExpression in the turbinate bone.
meckel's cartilage
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 moderate expression: see section 14 19 20
axial skeleton
strong strong
regionalstrong expression: see section 07 08 moderate expression: see section 10 11 12 13 14 17
basioccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 21 22 23 24 moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20
basisphenoid bone
strong strong
regionalstrong expression: see section 08 moderate expression: see section 09 10 11 12 13 14
temporal bone petrous part
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 21 22 23 24 moderate expression: see section 19 20
vault of skull
strong strong
regionalstrong expression: see section 01
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 21 22 23 24 moderate expression: see section 17 18 19 20
clavicle
strong strong
regionalstrong expression: see section 07 08
scapula
strong strong
regionalstrong expression: see section 01 02 03 04 07 moderate expression: see section 21 weak expression: see section 20
sternum
moderate moderate
regionalmoderate expression: see section 12
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 08
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31526
Entity Detected:Cebpz, CCAAT/enhancer binding protein zeta ( MGI:109386)
Sequence:sense strand is shown

>T31526
GCGTCCCAAACAGGTGACAGAAGATCCGGATGAGGAAGACGAGGAGGATGGCGATGAGGGCAAGAATGGC
TTCTCCCTGGAGGAGGTGTTACGCCTCGGAGGCACAAAGCAAGATTACCTTATGCTGGCTACTTTGGATG
AAAATGAGGAAGTGGTGGATGGGGGCAAAAAGGGAACCATTGACGACCTTCAACAAGGTGAACTGGAGTC
ATTTATTCAGAATCTGAATTTGGCCAAATACTCAAAGTCTTTGATTGAAGAAGATGAACCAGAGAAAAAG
GAAAACGCCAGCAAAAAAGAAGCAAAACTACTGAAGGTGGAGAATAAAAAACAAAAAGCAACAGAAGGAA
AAAAGACATCGGAGAAGAAGGTGAAAAATAAGACTGTAGCAGAACAGCGTCCTGAGAGCTGTCCCGTCTC
CAAAGCAAAGAAAGATAAACAGCCCGATGTGTTTGAATTTCTAGAGAGACAAACAATGTTACTCAGGCCT
GGAGGCAAGTGGTACGACATGGAGTACAGCGGTGAGTATTCCTTGGAGCCGCAGCCTCCAGATGTCGTGT
CCAAGTACAAAGCCCTGGCCCAGAAGCTGTATGAGCACGAAGTCAGCTTATTCAAGAGTAAGACAAACAA
TCAAAAGGGAGGCTCTTCCACCTGGATGAAGGCCATCGTGTCATCAGGGACACTAGCTGACAGGATGGCG
GCCATGATCCTTCTAATCCAGGATGATGCTGTCCACACTCTGCAGTTTGTAGAAACCCTGATGAGCCTTG
TGAAAAAGAAGGGCAGCAAGCAGCAGTGCCTCATGGCGTTGGACACGTTCAAGGAGTTGCTCATCACAGA
CCTTCTGCCAGACAGTCGGAAGCTCCGGGTTTTCAGCCAGCATCCTTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5352277), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 28534. Forward Primer - name:028534_F_IRAV67-70_C14_Cebpz, sequence:GCGTCCCAAACAGGTGAC; Reverse Primer - name:028534_R_SP6_IRAV67-70_C14_Cebpz, sequence:GGAAAGGATGCTGGCTGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18410 same embryo
 EMAGE:18411 same embryo
 EurExpress:euxassay_014370 same experiment
 MGI:4823798 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS