EMAGE mouse gene expression spatial database

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EMAGE:18847

Dock5 dedicator of cytokinesis 5 ( MGI:2652871)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014244. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014244_01	euxassay_014244_02	euxassay_014244_03	euxassay_014244_04

euxassay_014244_05	euxassay_014244_06	euxassay_014244_07	euxassay_014244_08	euxassay_014244_09

euxassay_014244_10	euxassay_014244_11	euxassay_014244_12	euxassay_014244_13	euxassay_014244_14

euxassay_014244_15	euxassay_014244_16	euxassay_014244_17	euxassay_014244_18	euxassay_014244_19

euxassay_014244_20	euxassay_014244_21	euxassay_014244_22	euxassay_014244_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18847_wholemount_strong.wlz

18847_wholemount_moderate.wlz

18847_wholemount_weak.wlz

18847_wholemount_possible.wlz

18847_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18847_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

renal cortex

weak

regional

weak expression: see section 05 06 07 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39640
Entity Detected:	Dock5, dedicator of cytokinesis 5 ( MGI:2652871)
Sequence:	sense strand is shown >T39640 GACGATACAGGAACAGAAAGGGGCGGATTCTGGCTGTAATCAATCTCTTCCCAAACAGGGATGGCAGAGA ACATGGATTAATGTCATGGTTCTTTTGCTTGTTTCTGTTACTTTTTTTGACGCAGAGTCTCACTATGTAG CCCAGGTTGGTTAGAACTAGTAGTGATCCCCCTGCCTCGGCTTTCCAGTTGCTGAGTATACAGGTACGAA CCCCCACATCCAGTAGAGTTCCTATGTCTCCTAGTCTTCCGCCTTCCGTTGCTCCAGGAAGCCAGTAGTT TTCCATGAAATCAGCAGCACAGTTTAAACGAGGCTCCATTTTGTTTCTCATCAAACCTCCAAGCTCTTAT ACATTTAACATGGCGTTAACCTGTGCTACAAGGAAGGAGCCCTGCTGACCAGGGAGCCCTGGATTTACTG CCCAGGGTTTCTACCTTAGCAGGACTGTCTGTGGTGGCATGCACTCACGGGATCAGGCCTGTTAGTGGGA GGGTGAGTTGGGGCAGAGAGGAACAGGATCGTTGCCTTAGGGATGGTGCACAGCAGAGAGAAGAAGGCAC ACTCAAGACTCAGATCCTGGTTGCTGTTTAGGAGTACCTAGTACCTAGTACTCCTAGGGTTGGTTTTGAC AAATTCTGGCTTTCGAGAGCGTATGGATGGTGTTTCAACTTGAACTCAAGGCTGGACTCAACAGACTAGA GTTCAGAGCTCATCCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 270422. Forward Primer - name:270422_F_cDNA_Dock5, sequence:GACGATACAGGAACAGAAAGGG; Reverse Primer - name:270422_N_SP6_cDNA_Dock5, sequence:TGGATGAGCTCTGAACTCTAGTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18849 same embryo
	EMAGE:18846 same embryo
	EMAGE:18848 same embryo
	EMAGE:18850 same embryo
	EurExpress:euxassay_014244 same experiment
	MGI:4824370 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source