EMAGE mouse gene expression spatial database

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EMAGE:18989

Luzp2 leucine zipper protein 2 ( MGI:1889615)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015946. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015946_01	euxassay_015946_02	euxassay_015946_03	euxassay_015946_04

euxassay_015946_05	euxassay_015946_06	euxassay_015946_07	euxassay_015946_08	euxassay_015946_09

euxassay_015946_10	euxassay_015946_11	euxassay_015946_12	euxassay_015946_13	euxassay_015946_14

euxassay_015946_15	euxassay_015946_16	euxassay_015946_17	euxassay_015946_18	euxassay_015946_19

euxassay_015946_20	euxassay_015946_21	euxassay_015946_22	euxassay_015946_23	euxassay_015946_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18989_wholemount_strong.wlz

18989_wholemount_moderate.wlz

18989_wholemount_weak.wlz

18989_wholemount_possible.wlz

18989_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18989_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 13 15

pons marginal layer

weak

regional

weak expression: see section 07 20

pons ventricular layer

strong

regional

strong expression: see section 09 19 moderate expression: see section 07 08 10 18 weak expression: see section 11 17 20

midbrain ventricular layer

strong

regional

strong expression: see section 09 moderate expression: see section 10 18 weak expression: see section 11 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63434
Entity Detected:	Luzp2, leucine zipper protein 2 ( MGI:1889615)
Sequence:	sense strand is shown >T63434 CCACACCTCCAGCTACATATCACCCCACCCCCTGTTTCATATAGTATGAAATTCTCTTAGTAGAATAATA CATCTATACTTTTCTATTTCTTTTTCTTTTGAAGGGAAACCTTTTTTGGAGTAAAAAATTATATATATGT GTGTGTCAAGTTTGGCTCAAAATTAATACAAAGTGGAGTATGACCTTAAGCTCTTTATCCTCCTGCATCT ATTTCCCAAGGGCTGAGAATTTCAGCCACTCACCATCACTACAGTACTGAAAAGTACCAGTTCAGTTTTC ATTTAAGATTACCTTTCCACTGTTATCTAGGGCATTGATTTCCTGCCTCATACCTGTGAATCCTGCAGGA TGCTAGTCACTTGTTCTTCGCATGGCTGGTTGTGAATATCTGTGACTCCATGTAGGAATGACTGGATAAG GAGTTCCAAGTTCAAGCAACCTTGTTTTCTAGTTTTGTGTTTTGTCTTGACTTTGACTTCCTGTTCTAGA ATGTGGTGAGATAAGCCAGAGTGCCTTTCTCCAGCTCGGGGAATGATGAAATGGCTTTTAATCAATAGGG GGAGATTTCCTACTAAAATATATGCGTTCTTGTGTATCTACAGGTTTTCTCTAGGTTGTGTGTCATACTA TTTACTACTTATATTTGAAAGAAAGACTGTAAGAGCAGGAGGTTTTATTCTAACCACCTTTAAGGACCAT GACAAAAGTTCATTTAGTACTTTGATAGGGCTGGTTTTAATATGAGTGAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95989. Forward Primer - name:095989_F_cDNA_Luzp2, sequence:CCACACCTCCAGCTACATATCA; Reverse Primer - name:095989_N_SP6_cDNA_Luzp2, sequence:CCTCACTCATATTAAAACCAGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18992 same embryo
	EMAGE:18990 same embryo
	EMAGE:18991 same embryo
	EurExpress:euxassay_015946 same experiment
	MGI:4826025 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source