EMAGE mouse gene expression spatial database

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EMAGE:19327

Cav3 caveolin 3 ( MGI:107570)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002047. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002047_01	euxassay_002047_02	euxassay_002047_03	euxassay_002047_04

euxassay_002047_05	euxassay_002047_06	euxassay_002047_07	euxassay_002047_08	euxassay_002047_09

euxassay_002047_10	euxassay_002047_11	euxassay_002047_12	euxassay_002047_13	euxassay_002047_14

euxassay_002047_15	euxassay_002047_16	euxassay_002047_17	euxassay_002047_18	euxassay_002047_19

euxassay_002047_20	euxassay_002047_21	euxassay_002047_22	euxassay_002047_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19327_wholemount_strong.wlz

19327_wholemount_moderate.wlz

19327_wholemount_weak.wlz

19327_wholemount_possible.wlz

19327_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19327_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 14 15 16 17 18 19 20 21 22 23

head mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 11 12 14 15 16 19 20 21 22 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

tongue

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4601
Entity Detected:	Cav3, caveolin 3 ( MGI:107570)
Sequence:	sense strand is shown >T4601 CCACGCGTCCGGCCCTGGTGGCTGGCAGGGAATGCCAGGTCTCCTCTCGCGTGAGGCCAATGTCCTGCAC AGCCCCCTTTAAGGAGCGTTCTGCCATCCAGTCTTAATTTTAGCTTGGAGGCGTGCCACCAGCAGCTTTA ACTAGAGGCCGGCTGAAGTTATGCAGGACAGCTGTCAGCCGGCATGCTGGGTGGGGCCTATTTAGCCGGC AGCGGCACCAGTCTGGAGACCCTAAGCTTGGTCTTTCTGCCCCCAGGACTATCAACGATACCAGCCACAA GGCTCTGATCGCCTCCTGAAGGAGCCCCAGCCTCACAATGATGACCGAAGAGCACACGGATCTGGAAGCT CGGATCATCAAGGACATTCACTGCAAGGAGATAGACTTGGTGAACCGCGACCCCAAGAACATCAATGAGG ACATTGTGAAGGTAGATTTTGAAGACGTGATTGCGGAGCCCGAGGGCACCTACAGCTTCGACGGTGTATG GAAGGTGAGCTTCACCACGTTCACCGTCTCCAAGTACTGGTGCTACCGCCTGTTGTCTACACTGCTGGGT GTTCCACTGGCCCTGCTCTGGGGATTCCTGTTCGCCTGTATCTCCTTCTGCCACATCTGGGCCGTGGTGC CCTGCATTAAGAGCTACCTGATCGAGATCCAGTGCATCAGCCACATCTACTCACTGTGTATCCGCACCTT CTGCAACCCGCTCTTCGCTGCGTTGGGCCAGGTCTGCAGCAACATTAAGGTGGTGCTGCGAAGGGAAGGC TAAAGCCAGGCTGGGCTGTGGGGAAGGCTGGGCAGGGGGATCAAAGGTCAGGTGAGCATACCTGGCTCTG CTCCACATGGGCTGCTGGAGAGCTCTTCCTTTTGAGGTTCCTCTCAATTCCACCTCAAGATGGGAAAACT TGGTGCGGGAGGAGCCAGAAGGAAAAGACAGCCCAGTGTGGAAACACGGGCACCCCTGCTCCGCTCAGCC TCCCATGAACCCCGGCGGCCCTGACTGAGCGGAGGGTTCCTTAAGAGGCAGCTAGCGCAAGGCTTTGCGT TCACATGTACTGTAATAGCCAGTCTTCTAGAGGTGTGACTCGTCTCCTCATGCTAGGGGGAGCAAGCAGT GACTGCCGGCATTGAGGAAGGTGCTGCAATAAAGGGTTTTAACTGCAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4988598 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4988598 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19329 same embryo
	EMAGE:19330 same embryo
	EMAGE:19328 same embryo
	EMAGE:19325 same embryo
	EMAGE:19326 same embryo
	EurExpress:euxassay_002047 same experiment
	MGI:4823649 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source