EMAGE mouse gene expression spatial database

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EMAGE:19329

Noxa1 NADPH oxidase activator 1 ( MGI:2449980)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002004. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002004_01	euxassay_002004_02	euxassay_002004_03	euxassay_002004_04

euxassay_002004_05	euxassay_002004_06	euxassay_002004_07	euxassay_002004_08	euxassay_002004_09

euxassay_002004_10	euxassay_002004_11	euxassay_002004_12	euxassay_002004_13	euxassay_002004_14

euxassay_002004_15	euxassay_002004_16	euxassay_002004_17	euxassay_002004_18	euxassay_002004_19

euxassay_002004_20	euxassay_002004_21	euxassay_002004_22	euxassay_002004_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19329_wholemount_strong.wlz

19329_wholemount_moderate.wlz

19329_wholemount_weak.wlz

19329_wholemount_possible.wlz

19329_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19329_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 07 08 09 10 12 moderate expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4598
Entity Detected:	Noxa1, NADPH oxidase activator 1 ( MGI:2449980)
Sequence:	sense strand is shown >T4598 CCCACGCGTCCGCCCACGCGTCCGCGGACGCGTGGGCGGACGCGTGGGGCGGGGCGTGAGAGAGGGGCCT AAGCCTTGGGCCATCTACTCAGTTTCAAGGACCGACTGTCACAACTCCAGTTGTCTTGCTACCAGCTGCT TCTCATTGCTGTCCTGGTCCCCTTGGCCCTGGCCTACGGCCTCCCAGCTTCTGTGGGGCACCCTCCTCCT GTCCCACGCGCTCCAGCTAGAATCGCTAACTTCCTTCGCCTCAAAGCAGGCGTCCCCGCTCCATGAGCTC TCTAGGGGATCAGATACGGGACTGGCACCGGGGTGTGCTGGCCGTGGCACGCGAAGACTGGGACTCTGCG CTGTGCTTCTTCTCAGATGTCCGAGAGCCGCTGGCTAGGATGTACTTTAACAGGGGCTGTGTGCATCTGA TGGCAGGGGATCCCGAGGCTGCGCTGCGGGCATTTGACCAAGCAGTGACTAAGGACACCTGCATGGCTGT TGGCTTCCTCCAGCGGGGAGTGGCCAATTTCCAGCTGCAGAGGTTCCAGGAGGCTGTGTCTGACTTCCAG TTGGCCCTGGCACAGCTGAGGGACAATGCTGTCATTGACTACACACAACTGGGTCTGAACTTCAAATTGC AAGCCTGGGAGGTCCTATACAACATGGCATCAGCACAGTGCCAGGCAGGGCTCTGGACCAAGGCTGCCAA TACTCTAGTGGAGGCAATCTCCAAATGGCCAGAGGGGGCTCAAGACATCCTGGACATTGCCATGGACAAA GTGCAGAAACAGGTACCCCTACAGCTACAGCAAGTGCCCAAGGGTGAGGTCTTCCAGCCTCCCAGGCGAT ACCTAAAACATCTGGAGCCCATGGATTTCCTTGGCAAGGCTAAGGTGGTGGCTTCTGTCATTCCTGATGA CCACAACGCCCAGCCTCAGCAGAGGTCCCAGGCGGAGCATGCTGGCCACCAGCCATCCTCATCTACGTGT AAGAGGGTCCTGAGCACTACGGGTGGTCACACGAGCCCTGGCCTATATGATAGTTTGCTGGCATCTGGAA GGCCTGGTCCAGGCCCCTCTGAAGTTTCCTCAGGATCTGAGGGAGCAGCTACAAAGGACCCTGAATCCTT GGTGACTGTCACTGTGCAGTGCCACTTTACTGTGCCCCTGAAGGTCCCAAGAGGAACTGGCCTGTCCAGT TTTCAGACACTACTAGCTCAAGCCCTCCTTCACCAGACGCAGACAGGGCAGCTCAGTTACAAAGCCCCAG GAGAGGAGAGATCCTGGATTCCCATCTCCACGGAGGAGTCCCTGCAGAGTATATGGAGGAATGTGCCCGT GGGCCCAGGAGGGTTGCAGCTCCAGTGCCAGGGGGTCTGGGGCCGGCCAGTCCTCTACCAAGTAGTAGCT CAGTATAACTATCGTGCCCAAAGACCGGAGGATTTGGACTTCCACCAAGGGGACACGGTGGATGTTCTGT GTGAAGTGGACGAAGCATGGCTGGAGGGACACCGAGATGGCTGCGTTGGCATTTTCCCTAAGTGCTTTGT GGTCCCAGCTGGCGCCTATGTGGAAGCCATGCTTGTACTGGGACCCCAGCCAGGAGACCAGAACTAGCAA TAAATACCATGACAAAACCAGCTTTAATTAAACAAGCAAACAAGCAAACAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4988389 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4988389 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19330 same embryo
	EMAGE:19328 same embryo
	EMAGE:19325 same embryo
	EMAGE:19327 same embryo
	EMAGE:19326 same embryo
	EurExpress:euxassay_002004 same experiment
	MGI:4826756 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source