EMAGE mouse gene expression spatial database

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EMAGE:19348

Trappc4 trafficking protein particle complex 4 ( MGI:1926211)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002113. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002113_01	euxassay_002113_02	euxassay_002113_03	euxassay_002113_04

euxassay_002113_05	euxassay_002113_06	euxassay_002113_07	euxassay_002113_08	euxassay_002113_09

euxassay_002113_10	euxassay_002113_11	euxassay_002113_12	euxassay_002113_13	euxassay_002113_14

euxassay_002113_15	euxassay_002113_16	euxassay_002113_17	euxassay_002113_18	euxassay_002113_19

euxassay_002113_20	euxassay_002113_21	euxassay_002113_22	euxassay_002113_23	euxassay_002113_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19348_wholemount_strong.wlz

19348_wholemount_moderate.wlz

19348_wholemount_weak.wlz

19348_wholemount_possible.wlz

19348_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19348_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 13 14 weak expression: see section 12

brain

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 06 07 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 18

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 18 19

spinal cord

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 15

cervical ganglion

moderate

regional

moderate expression: see section 09 16 17

thoracic ganglion

moderate

regional

moderate expression: see section 11

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 13 14 15 16 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 weak expression: see section 12 13 15 16 17 18 19

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 15 16

lower jaw molar

moderate

regional

moderate expression: see section 09

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 16

upper jaw molar

moderate

regional

moderate expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5497
Entity Detected:	Trappc4, trafficking protein particle complex 4 ( MGI:1926211)
Sequence:	sense strand is shown >T5497 CGTAAAATTGCTGCACTTTGCTGCGGGTCACCCAACAGCCACTAATTCTCTGCTCTGTCACTGAGAAATA CAGTAGGCCAGTTCCCAGAAATGTGTTTGTTAAAGCAATGGGGGCCTTGGCTTGCGCGTGCGCTTTAGGA GGGGGGAGGGCCGCAGGAGGGAGAGCAGAGGCGAGGCTATGACACCAGTTCCGGGACTGGAGCGCCTGGA AGGGGCGGGATCGTAGTACCCTATCCGTAGCACAGTGATGGCGATTTTTAGCGTGTACGTGGTGAACAAA GCCGGCGGCCTGATTTACCAGTGGGACAGCTATTCGCCACGGGCTGAGGCTGAGAAGACTTTCAGTTACC CGCTGGACCTGCTGCTGAAACTGCACGACGAACGCGTGCTGGTCGCTTTCGGCCAGCGCGACGGCATCCG GGTGGGCCATGCAGTGTTGGCCATCAATGGCATGGACGTGAATGGCAAGTACACGGCTGACGGGAAAGAA GTGCTGGAGTATCTGGGTAACCCTGCGAATTACCCGGTTTCCATCCGATTCGGCCGGCCCCGCCTTACCT CTAACGAGAAGCTTATGCTGGCCTCGATGTTCCACTCGCTCTTTGCCATTGGTTCCCAGCTATCTCCGGA ACAGGGCAGTTCAGGCATTGAGATGCTGGAAACAGACACCTTCAAACTGCACTGCTTCCAAACACTGACA GGGATCAAGTTTGTGGTCCTGGCAGACCCTAGGCAAGCTGGAATAGATTCTCTTCTCCGAAAAATTTATG AAATTTACTCAGACTTTGCCCTCAAGAATCCATTCTACTCCTTGGAAATGCCTATCAGATGCGAGCTGTT TGATCAAAACCTGAAGCTAGCTCTAGAGGTGGCAGAGAAGGCTGGCACTTTTGGACCTGGGTCATAGGAT GAACCTGGAATGGACGCCCTAAATTCTGAGACGTGCTCCAGAGGGCTTTGCTGTGTCTACTCGGTGAGGG TCCTGTTGTAGCAGCCTTGCTAATATACTTGAAAATGGTAGACCACTGAGCCTGGTGATGGTGACAGATC CTGGGCTCGAATGCTGTGTTCTCTGCCCCTCTGTATATATTACAGTCCTACTCTGTATAGATTTATTTAT GGAGGAAACTGGCTTAGCAATGCTGAAATAAACATTCCTTTGTTTTAGAAAAAAAAAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5401311 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5401311 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19352 same embryo
	EMAGE:19351 same embryo
	EMAGE:19350 same embryo
	EMAGE:19353 same embryo
	EMAGE:19349 same embryo
	EurExpress:euxassay_002113 same experiment
	MGI:4828898 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source