EMAGE mouse gene expression spatial database

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EMAGE:19350

Capn9 calpain 9 ( MGI:1920897)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002099. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002099_01	euxassay_002099_02	euxassay_002099_03	euxassay_002099_04

euxassay_002099_05	euxassay_002099_06	euxassay_002099_07	euxassay_002099_08	euxassay_002099_09

euxassay_002099_10	euxassay_002099_11	euxassay_002099_12	euxassay_002099_13	euxassay_002099_14

euxassay_002099_15	euxassay_002099_16	euxassay_002099_17	euxassay_002099_18	euxassay_002099_19

euxassay_002099_20	euxassay_002099_21	euxassay_002099_22	euxassay_002099_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19350_wholemount_strong.wlz

19350_wholemount_moderate.wlz

19350_wholemount_weak.wlz

19350_wholemount_possible.wlz

19350_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19350_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5501
Entity Detected:	Capn9, calpain 9 ( MGI:1920897)
Sequence:	sense strand is shown >T5501 GGGGAAGCTGTCATGCCTTACCTGCATCGGTCCCTGAGGCCACAGCCACAGCCAGTCCCCGGGGATGCCA GGACTATCCACTCCTCAGGTCAGAGCTTTGAGCAGCTGAGGCAGGGCTGCCTGCAGTCTGGCACCTTGTT TGAGGATGCTGACTTCCCTGCCAGCAATGTCTCCCTGTTCTACAGCGAGAGGCCCCAGGTCCCCTTTGTG TGGAAACGGCCAGGGGAAATTGTGGAAAAACCAGAATTCATTCTTGGAGGGGCCACCAGGACCGACATTT GCCAAGGGGAGCTGGGAGACTGCTGGCTCCTGGCAGCCATTGCCTCCCTAACCCTCAATCAGAAAGCACT GGCCAGGGTGGTTCCCCAGGACCAAGGATTTGGTTCTGGCTATGCCGGGATATTTCATTTCCAGTTCTGG CAGCACAGTGAGTGGCTGGACGTAGTGATTGATGACCGCCTGCCCACCTTCAAGGACCGCCTGGTCTTCC TCCACTCTGCTGATCACAATGAGTTCTGGAGCGCACTGCTGGAGAAAGCCTATGCCAAGCTTAACGGGAG TTATGAGGCACTGAAGGGAGGCAGTGCCATTGAAGCCATGGAAGACTTCACTGGGGGTGTGGCTGAGAAC TTCCAAATCCGAGAGGCACCAGAGGATTTCTTTGAGATTCTGGAGAAGGCTTTGAAGCGTGGTTCCTTGC TGGGATGCTCCATAGATACCCTGAATGCCTCAGAATCTGAAGCTCGAACATCTCTTGGTCTCATTAAGGG CCATGCCTACACCGTGACGGGGCTCGATCAGGTAAACTTCCATGGCCAGAGAATCAAGCTCATCAGAGTC CGTAACCCTTGGGGCCAGGTGGAGTGGAACGGGCCATGGAGTGACAGCTCTCCTGAGTGGCGCTCTGTGG ACCTGGAGGAGCAGAAGCGCCTGGGCCACACTGCCCTTGATGACGGGGAGTTCTGGATGGCATTCAAAGA CTTCAAGATCCATTTTGACAAAGTGGAGATCTGCAACCTCACACCTGATGCCCTGGAGGACAGCGCCCTC CACAGGTGGGAGGTGACCATCCACCAAGGAAGCTGGGTCCGTGGCTCCACTGCTGGTGGCTGTCGCAACT TCCTGGACACCTTCTGGACCAACCCTCAGATAAAGCTGTCCCTGACCGAGAGGGACGAGGGCCAGGAGGG CTGCACTTTCCTAGCAGCCTTGATGCAGAAAGACCGCAGGAGGCTCAAGAGGTTTGGCGCCAACATGCTG ACCATTGGCTACGCCATTTACCAGTGCCCAGACAAGGACGGACACCTGAGTAGGGACTTTTTCAGGTACC ATGCCTCCCTGGCCCGAAGCAAGACGTTCATCAACTTGAGAGAAGTCTCAGAGCGATTCCAGCTGCCCCC GGGAGACTATATCCTCATCCCCAGCACCTTTGAGCCGCATCAGGAGGCCGACTTCTGCCTGAGAATCTTC TCTGAGAAGAGAGCTGTGACTCGGGACCTGGACGAGAACATAGACATTGACCTTCCTGAGCTTCCAAAGC CGACTCCACAGGAGGAGGAAACAGAAGAGGAACAGCAGTTCCGGGCCCTGTTCCAGCGAGTTGCTGGCGA GGACATGGAGGTGTCAGCTGAGGAGCTTGAATATGTTCTAAATGCTGTTCTGCAGAAGAAAACAGCCCTC AAGTTCAAGAGGCTGAGCCTGCTGTCCTGCAGAAACATCATCTCTCTGATGGATACCAGTGGCAACGGGA AGCTGGAGTTTGAAGAGTTCCGGGTCTTCTGGGACAAGCTGAAACACTGGATGGACCTGTTTCTCCAGTT CGATGTGGACAAGTCTGGCACCATGTCTTCCTATGAGCTGCGGACGGCACTGAAAGCTGCAGGCTTTCAG CTGGGTGGCCATCTCCTGCAGCTGATTGTCCTCAGGTATGCAGATGAGGACCTCCAGCTGGATTTCGATG ACTACCTCAACTGCCTGGTGCGGCTGGAGAATGCAAGCCGGGTGTTCCAATCTCTCAGTGTGAAGAACAA GGACTTCATCCATCTCAACATAAACGAGTTCATCAGCCTGACCATGAACATCTGAGGTTGCTCTGACCCG ATGCCGTCTGCCCCTCTGATACTCAGCTTCTGGATCATGAACCTCGAAACTTTTTTTAGAGGGCTGCCCC GCCCACTGCTCTCTCTCCTCCCGGCCTCACACCACCCTCCTCCCCTCTCCATAGTGAGTTAGAAGAATCT AAAGGGATTGGCTGAGTTCTCTGATTCTGTGGAACTTCTTATAAAATCATTGCTCTACAAGAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6394969 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6394969 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19352 same embryo
	EMAGE:19351 same embryo
	EMAGE:19353 same embryo
	EMAGE:19349 same embryo
	EMAGE:19348 same embryo
	EurExpress:euxassay_002099 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source