EMAGE mouse gene expression spatial database

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EMAGE:19353

Cdc42se2 CDC42 small effector 2 ( MGI:1919979)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002100. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002100_01	euxassay_002100_02	euxassay_002100_03	euxassay_002100_04

euxassay_002100_05	euxassay_002100_06	euxassay_002100_07	euxassay_002100_08	euxassay_002100_09

euxassay_002100_10	euxassay_002100_11	euxassay_002100_12	euxassay_002100_13	euxassay_002100_14

euxassay_002100_15	euxassay_002100_16	euxassay_002100_17	euxassay_002100_18	euxassay_002100_19

euxassay_002100_20	euxassay_002100_21	euxassay_002100_22	euxassay_002100_23	euxassay_002100_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19353_wholemount_strong.wlz

19353_wholemount_moderate.wlz

19353_wholemount_weak.wlz

19353_wholemount_possible.wlz

19353_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19353_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5500
Entity Detected:	Cdc42se2, CDC42 small effector 2 ( MGI:1919979)
Sequence:	sense strand is shown >T5500 CGGCTGGAGCAGGCGGCGGAGACAAAGGCGACGATAGGGCCAGTGTTGAGTGAAACAAACGCAGAAACCA AAAGAGAAGACGTCTTCAAGGAACAACTTATGCTAATGAGTCTGAAGAGTCTCCTGTGTGTGTCTTTCAG CATAGTCTCCATTCAGAGAGCTGAGTGAGTGGCCACATTGATGAGTGACCTGCTGCCGTCTCTGAACCAA AGCTGTTCTTTCCTTCCCACTGACCTGGAACCAGTGTCTGTAGAAACGGAAGCAGAGAAAAGAAACAATC AGACATCCCTTTCAATCAACTTGAGACATTTGACTCCAGGGACGGAGGCACGGTGAAATAAAGTTATTTT GGCGACATTCCTTGTGGAGCTTCCTGTCTGAAGACTTGGAGCCCTCGGTGGTGCTCGCTCAGGCCTCGGA CTGAGAGCATGAGTGAATTCTGGTTGTGTTTCAACTGCTGTATTGCAGAGCAGCCTCAGCCTAAGAGGCG ACGACGGATTGACCGAAGTATGATCGGAGAGCCAACCAACTTTGTGCACACAGCTCACGTAGGATCCGGA GACCTATTTAGTGGGATGAACTCAGTCAGCTCCATTCAGAACCAGATGCAGTCCAAGGGAGGCTATGGAG GTGGAATGCCTGCCAACGTGCAGATGCAGCTCGTGGACACCAAGGCAGGATAGCCCTGGTCCCTGCACTG TCATCGTGAATTCCTGTATTTTCTCTGTCCACAGTTCTATAATATTTTTGTCTTGTGATTGGATTTCTTT TATTTTAATTATAAAAAAGAAGTGTGGCGGCAATCTGTTCACCCCTGTGATCTCCAGCAGAGACGTTCCC GCTCCAAGGAAGGCAGCGTCAGGCCGACCTGCCTTGCCGCCAGCGGCATGCATGCCTGGGACTCTGACAG AGTTCTTTGGATCTTAGCTGAATTTTCAATGTTTAATTGACATGGATCGGATCTTAGCATGGTCTTTTCT GTGACTGCTAGCACTGTATTGTAATTTTTCCATTTTAAGCCTTTTCATTGCACCTCCAGCCCCTCTGCCT TATGATTTAACTGGTAAACAAAGACCTGTAATTACTGCCACCATTTATGTATATTTTGAAGGTATGAGAC CCACAAGCACAATAGTCCTTTTCATCTGTTGGAAACTTGAGCGGAGCGCGTCTGCACACTCCATGGTGCT GCTCAGAGGGGACCCCATGGGATGCCAGACATCCATCCTGAGCTGCTGTGGCCTGAGGATGCTCCTCTTT CAGAGGAGGCTCTTTACAGATATTCCAAATGGGTTCTGGTCCGACTAGAGCAGTGAAAGGAAAGCCTGCG TTCAGAGGCGGTTGCTTGGCTGTAAATGTTCAGTGTATTTATTTGAGAGTAAGGACCTGTGATTGTGAGC AGATATAGCCTGTATCTGAGTGTTGTGAAGCCCATTCAGTTTCTCAGCATCTTCGTCCTGTTAGCTCTTC TTTACGTACCTGTTGCTGTTTTCTATTTAGTCATTTGGTGTCAAATTGTTTCCACTAGCTGTGTGTTGAA ATTCTGGCCTCTGCTACCAGCTGTGACTTGCAGAGTCCTTGGCCTTCCAGTGTTCCACTTACTGTGGAGG CTGTGGCAGCTTCCAACAGCCACCTGCTGCCTGGCTGATTCACAGTGATCAGCCCTCACAGTGACACTCT GTGGTTAGTAAGAGGCCTTTGCTCAGGTCCCATTCTGTTCCTCTTCCCTGCTGAGTATAGGTAGCTGGAT CCTGCGGTCCACTTTGCTTACTATCTAACTGCCTTTGATTGATTGATTGATTGATTGATTAGCATTTGTG CAGAATATGGATCTTAACTGAGATTAGAGTGCTGAAGGCATGTAGGTCCTTGTCTAGAGAAGCTGTGTTC TTTTCTGCCTTTGCCTGCCCCTGTCCTGAGAATGCTGACTGAAGAGCCACTGAAGGCTGACGCAGGCAAA GCTGAAAGCATTTCTCCTATATTTCTTCTGAACATAACTTAGCAGTAGGGTGGAACACTGGATGACCTGA TTCTTTGATTTTGCAATTCTTACCGTTCTTAGGTGTCAATCCTGAGCACAGAGAATTTCTCTGTCAAGGA GAACCATTTTCTCACTGTTGTCTTTCATTTACCGTATGGACACTGCACTCTAGAGTCTGTCATTCTGCTT AGCCAGGGTGAGGTTCCTGCCAGGGCACTGCTGTGTGCAGACTCTTTCTGTATAACAGACAGGGATTTCT TGGCATCATTGGCACTGATAGATGGGACAGCACAGGGAGACATTGTCCCCTCCCCCATCACTGTTTGCTG CTCCAAGGCCAGTCATTAATAAGCCAAAGGCTCCTGAAGCCCCTGATGGTCCTGGCAGCCTCTGACCTCT CAGCACAGTGAGACATTGTACACAGTGCACCGCCTGGTGCCCTGGAGGGTTCCAATAAGGTCAAGAGAAT GTACAGAGTAACCTGGTACATGGTGTTTATGGAGGCCTAAGGATGCCGGCAAAGGTGCAGGGAGACATAC TTCCCACCTCCCCACATGGGATTGTAAACCTTGGGAACTCCTTTGTTCCCCCAGTCACACATCTCATTTT TAAATTGGAGCTAATAGGATTTTGTACCCAGAATTTATTATATAGTTAATACTTCTCTGAAATACATGGT AACAACTAGTAAGCAAGAGATACCGCAGATTGCAGCAGTGTGCTGGGCACAGCTTCTCCAGTGGGCCTGG AGAGTTTGTCCATTAGCTTCCCTGTGGTCATTAAATGGAGCTGCTAGGGTAGAGCAGTGGCCTGATCTGC TCAGTGACTTGTGCTCAGCACCAGAAGACAAGATGGACGTATTTTTATAATCTAAGCATACTTTTTTTGT ACATTGTGTTCATTCTTGAATAAAGTGAGCTCACTGTTGGCTTGTAGATATTAAAAAAAGGAAAGTATTG ATTTTGATTCAATAAATGTTTTCTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6394856 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6394856 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19352 same embryo
	EMAGE:19351 same embryo
	EMAGE:19350 same embryo
	EMAGE:19349 same embryo
	EMAGE:19348 same embryo
	EurExpress:euxassay_002100 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source