EMAGE mouse gene expression spatial database

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EMAGE:19372

Crip2 cysteine rich protein 2 ( MGI:1915587)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002192. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002192_01	euxassay_002192_02	euxassay_002192_03	euxassay_002192_04

euxassay_002192_05	euxassay_002192_06	euxassay_002192_07	euxassay_002192_08	euxassay_002192_09

euxassay_002192_10	euxassay_002192_11	euxassay_002192_12	euxassay_002192_13	euxassay_002192_14

euxassay_002192_15	euxassay_002192_16	euxassay_002192_17	euxassay_002192_18	euxassay_002192_19

euxassay_002192_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19372_wholemount_strong.wlz

19372_wholemount_moderate.wlz

19372_wholemount_weak.wlz

19372_wholemount_possible.wlz

19372_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19372_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 03 12

trigeminal v ganglion

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 05 06 14 15 16 17 18

vagus x ganglion

moderate

regional

moderate expression: see section 04

dorsal root ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 07 09 10 11

heart ventricle

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6038
Entity Detected:	Crip2, cysteine rich protein 2 ( MGI:1915587)
Sequence:	sense strand is shown >T6038 CGGACGCGTGGGTCCGCGGACCGACCGAGCGCACCGACCATGGCCTCCAAGTGTCCCAAGTGTGACAAGA CCGTATACTTCGCTGAGAAGGTGAGCTCCCTGGGCAAGGACTGGCACAAGTTCTGTCTCAAGTGTGAGCG CTGCAACAAGACACTGACCCCCGGCGGCCATGCTGAGCATGATGGGAAGCCCTTCTGCCACAAGCCCTGC TATGCCACACTGTTTGGACCCAAAGGCGTGAACATCGGGGGCGCTGGCTCCTACATCTACGAGAAGCCTC AGACCGAGGCCCCTCAGGTCACTGGCCCCATCGAGGTCCCTGTGGTGAGAACTGAGGAGCGAAAGACCAG CGGCCCCCCCAAGGGTCCCAGCAAAGCCTCTAGTGTCACCACATTCACTGGGGAGCCCAACATGTGTCCT CGATGCAACAAGAGAGTGTACTTCGCTGAGAAGGTGACCTCTCTGGGCAAGGACTGGCACCGGCCCTGCC TGCGCTGTGAGCGCTGCTCCAAGACCCTGACCCCAGGCGGGCATGCTGAGCACGATGGCCAGCCCTACTG CCACAAGCCTTGCTATGGAATACTCTTTGGACCCAAAGGAGTGAATACTGGTGCTGTGGGCAGCTATATC TACGACAAGGACCCGGAAGGCACAGTTCAGCCCTAGATCTGCAGATGCTGTCCTCGGGGTCCCCCTGTTT GACCCGGAGGCAAAGTGGCCTGTTGCCTAGTCCTGCCTCAGCGTGTCTCGCCTGCAAATCCGGGACCTAA GTGGTGGAGGAGAAAGCCTGGATAGTCCCAGAGCTTCAGCCCCCTTTGTCACCTTGGCGTGTCCCGTGCT GCCCACCGTTTACTTCCTGTCTGTGTGCCTCCGTAGCCCCATGGGTCCTGTGTTCCTGTGTCCCTGATAG CTCTCCAAGGTGACTGTCCTATGATATATCCCTTTGCCCACACCTGCCCACCAGTATTATTTATGCTCTG CTTGCCGGTGATGGCCGTGAGCTCACAGCATTCCCAGGGTGATGGCTGGTGCCCTTGCGAGGAGCCCTCT GCTGGTTCCACACTACTCCCTACCTACCCTCACATGGTTCATGGCTATGGAGACTTTTGCTGTCAATAAA TAGTTTGGTTTGAGGATTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3489018 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3489018 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19376 same embryo
	EMAGE:19375 same embryo
	EMAGE:19373 same embryo
	EMAGE:19374 same embryo
	EMAGE:19371 same embryo
	EurExpress:euxassay_002192 same experiment
	MGI:4824055 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source