EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19374

BC016548 cDNA sequence BC016548 ( MGI:2448478)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002188. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002188_01	euxassay_002188_02	euxassay_002188_03	euxassay_002188_04

euxassay_002188_05	euxassay_002188_06	euxassay_002188_07	euxassay_002188_08	euxassay_002188_09

euxassay_002188_10	euxassay_002188_11	euxassay_002188_12	euxassay_002188_13	euxassay_002188_14

euxassay_002188_15	euxassay_002188_16	euxassay_002188_17	euxassay_002188_18	euxassay_002188_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19374_wholemount_strong.wlz

19374_wholemount_moderate.wlz

19374_wholemount_weak.wlz

19374_wholemount_possible.wlz

19374_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19374_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 11 12 13 14 moderate expression: see section 10 15 16

pancreas

strong

regional

strong expression: see section 05 06 07 10 11

cochlea

strong

regional

strong expression: see section 03 04 11 12 13

lower jaw molar

strong

regional

strong expression: see section 05 15

upper jaw incisor

strong

regional

strong expression: see section 08 09 13 14

upper jaw molar

strong

regional

strong expression: see section 05 15

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6039
Entity Detected:	BC016548, cDNA sequence BC016548 ( MGI:2448478)
Sequence:	sense strand is shown >T6039 CAGAGCTTGGATGTGAACCATGCTGTTTACCTGGTTGCTGGGAACAGCTATCACGTGAGGGCATGTGTGT GTGTGTTCTACCTGTCCTCCCTTGATACTCCTGAGGTAGCTGGTCCTGTGCCCAACTTTGATTCATTTCT AGTGAAAAACAACCTGACCTGAAGATAAGAATTCACTCCAGCAAGATCATCAGCTATGATAACTGGACCT CTCAGAGTTCATGTTAACTGGTGACTTATTGCACTCAAGAGTCCTTATGTGTGAGAAACAGGAGTCTAGC ATGGCTGTCCTCTGAGAGGCTCCACCCAGCAGCTGACTCAGAGAGATGCAGACACCCACAGCCAAACAGT GGATGGAGCTTGGGGACTCTTATGGAAGAATAGGAGGAAGGTTTGCATCCCCTAAGGGGATAGAACTCTA CAGGAAGACCAACAGAGTCACCTATCCTGGACCCTTGGGGCTCTCAGAGACAGAACCACCAACTACAGAA CATACATGGACTGGACCTAGGCCTTCCTGCACATATGTAGCAGATGTGCAGCTCGGTCTTCATCCCAAAC AACTGGAGTGTGGGTTATCCCAAAAGCTTTTGCCTGTATGTGAGATATGTTCTATAATCTGGGCTGTCTT CTATGGCCTCAGTGGGAGAGGATGCATCTAGCTTCACAGAGACTTGATATGCTCTGTTGGGGTTGGGGGC TCAGAAGGGGAGGGGAGATTTGTGGAAAGGGGACTGGGAGGGGGACAGTGAGTAGGATGTAAACTGAATA GGTAAAATAATAATAATAATGATAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3489202 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3489202 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19376 same embryo
	EMAGE:19375 same embryo
	EMAGE:19373 same embryo
	EMAGE:19372 same embryo
	EMAGE:19371 same embryo
	EurExpress:euxassay_002188 same experiment
	MGI:4823410 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source