EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19435

Fh1 fumarate hydratase 1 ( MGI:95530)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004825. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004825_01	euxassay_004825_02	euxassay_004825_03	euxassay_004825_04

euxassay_004825_05	euxassay_004825_06	euxassay_004825_07	euxassay_004825_08	euxassay_004825_09

euxassay_004825_10	euxassay_004825_11	euxassay_004825_12	euxassay_004825_13	euxassay_004825_14

euxassay_004825_15	euxassay_004825_16	euxassay_004825_17	euxassay_004825_18	euxassay_004825_19

euxassay_004825_20	euxassay_004825_21	euxassay_004825_22	euxassay_004825_23	euxassay_004825_24

euxassay_004825_25	euxassay_004825_26	euxassay_004825_27	euxassay_004825_28

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19435_wholemount_strong.wlz

19435_wholemount_moderate.wlz

19435_wholemount_weak.wlz

19435_wholemount_possible.wlz

19435_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19435_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23 24 25

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6529
Entity Detected:	Fh1, fumarate hydratase 1 ( MGI:95530)
Sequence:	sense strand is shown >T6529 GCACCATGTACCGCGCACTCCGTCTCCTCGCGCGCTCGCGTCGCCTCCTGCGGGTTCCGTCCGCCGGTGC TGCAGTGTCTGGGGAAGCGACCACCCTCCCGCGGTGTGCTCCGAACGTCGCGCGAATGGCAAGCCAAAAT TCCTTCCGTGTAGAGTTCGACACCTTTGGTGAATTGAAGGTTCCAACCGATAAGTATTATGGTGCTCAGA CCGTAAGATCTACGATGAACTTTAAGATTGGAGGTGCTACAGAACGTATGCCAATCCCAGTCATTCAAGC TTTCGGCATCTTGAAGCGAGCTGCTGCTGAAGTAAACCAGGAGTATGGTCTCGATCCAAAGATTGCTAGT GCAATAATGAAGGCCGCAGATGAGGTAGCTGAAGGTAAATTAAATGATCACTTTCCTCTGGTGGTTTGGC AGACTGGATCAGGAACCCAGACAAACATGAATGTAAATGAAGTCATTAGCAACAGAGCAATTGAAATGTT AGGAGGTGAACTTGGCAGCAAGAAGCCTGTGCACCCCAATGATCATGTTAACAAAAGCCAGAGCTCGAAT GACACCTTTCCCACAGCAATGCATATTGCTGCTGCAGTGGAAGTTCACAAGGTCCTGTTGCCAGGGTTAC AGAAGCTCCATGATGCTCTCAGTGCAAAATCCAAAGAGTTTGCGCAGGTCATCAAAATTGGGCGAACTCA CACGCAGGACGCTGTCCCTCTTACTCTTGGACAGGAATTCAGTGGTTACGTTCAGCAAGTCCAGTATGCG ATGGTGAGAATAAAAGCCGCCATGCCAAGAATCTACGAGCTCGCTGCTGGAGGCACTGCTGTGGGGACGG GGTTAAACACCAGGATCGGCTTCGCAGAAAAGGTGGCCGCAAAAGTAGCAGCACTCACAGGTTTGCCTTT TGTCACTGCCCCGAATAAATTTGAAGCTCTAGCTGCTCATGATGCTCTGGTTGAGCTTAGTGGAGCCATG AACACTGCCGCCTGCAGTCTAATGAAGATAGCGAATGATATTCGCTTCCTGGGTTCTGGTCCTCGGTCAG GTTTGGGAGAGCTGATCTTGCCTGAAAACGAGCCAGGGAGCAGCATCATGCCAGGAAAGGTGAACCCTAC TCAGTGTGAAGCGATGACCATGGTTGCAGCCCAAGTCATGGGGAATCACGTTGCTGTTACTGTTGGAGGC AGCAATGGACATTTTGAACTGAATGTGTTTAAACCAATGATGATTAAGAATGTGTTGCACTCAGCGAGGC TACTGGGAGATGCTTCAGTGTCCTTCACAGAAAACTGTGTGGTCGGGATCCAGGCCAACACAGAGCGGAT CAACAAGCTAATGAATGAGTCTTTAATGTTGGTCACAGCTCTTAATCCACATATAGGGTATGACAAAGCA GCAAAGATTGCCAAGACTGCGCACAAGAACGGATCAACCTTAAAGGAAACGGCTATTGAACTTGGCTATC TCACAGCAGAGCAGTTTGATGAGTGGGTGAAACCCAAGGACATGCTGGGTCCAAAGTGACTTCAATAAAT TTATATTAAAATAAACTTAAGAAATTTACAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3590691 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3590691 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19439 same embryo
	EMAGE:19436 same embryo
	EMAGE:19438 same embryo
	EMAGE:19437 same embryo
	EMAGE:19434 same embryo
	EurExpress:euxassay_004825 same experiment
	MGI:4824854 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source