EMAGE mouse gene expression spatial database

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EMAGE:19439

Btrc beta-transducin repeat containing protein ( MGI:1338871)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004824. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004824_01	euxassay_004824_02	euxassay_004824_03	euxassay_004824_04

euxassay_004824_05	euxassay_004824_06	euxassay_004824_07	euxassay_004824_08	euxassay_004824_09

euxassay_004824_10	euxassay_004824_11	euxassay_004824_12	euxassay_004824_13	euxassay_004824_14

euxassay_004824_15	euxassay_004824_16	euxassay_004824_17	euxassay_004824_18	euxassay_004824_19

euxassay_004824_20	euxassay_004824_21	euxassay_004824_22	euxassay_004824_23	euxassay_004824_24

euxassay_004824_25	euxassay_004824_26	euxassay_004824_27	euxassay_004824_28

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19439_wholemount_strong.wlz

19439_wholemount_moderate.wlz

19439_wholemount_weak.wlz

19439_wholemount_possible.wlz

19439_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19439_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 28 moderate expression: see section 22 23 24 25 26 27

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 18 19 20 21 22 23

spinal cord

strong

homogeneous

strong expression: see section 10 11 12 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 09 17 18 19 moderate expression: see section 08 10 11 12 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6077
Entity Detected:	Btrc, beta-transducin repeat containing protein ( MGI:1338871)
Sequence:	sense strand is shown >T6077 GCGATTATGGACCCGGCAGAGGCGGTGCTGCAGGAGAAAGCGCTTAAGTTTATGAATTCCTCAGAGAGAG AAGACTGTAATAATGGCGAACCCCCTAGGAAGATAATACCAGAGAAGAATTCACTTAGACAGACTTACAA CAGCTGTGCCAGGCTTTGCATAAACCAAGAGACAGTATGTCTAACAAGCACTGCTATGAAGACTGAAAAT TGTGTGGCCAAAGCCAAACTTGCCAATGGCACTTCCAGCATGATTGTGCCCAAGCAGCGGAAACTCTCAG CAAGCTATGAGAAGGAAAAGGAGCTGTGTGTCAAGTATTTTGAGCAGTGGTCAGAGTCTGATCAAGTGGA ATTTGTAGAACACCTTATATCCCAAATGTGTCACTACCAGCATGGGCACATCAACTCCTACCTAAAACCT ATGCTGCAGAGGGATTTCATAACTGCACTGCCAGCACGGGGTCTGGACCACATCGCTGAGAACATTCTGT CATACTTGGACGCCAAGTCACTGTGTGCTGCTGAGCTCGTGTGCAAGGAATGGTACCGCGTGACGTCGGA CGGCATGCTGTGGAAAAAGCTCATCGAGAGGATGGTCAGGACGGACTCTCTGTGGCGAGGCCTGGCAGAG CGCAGAGGCTGGGGACAGTACTTATTCAAAAACAAACCTCCTGATGAGAACGCTCCTCCCAACTCCTTTT ATAGAGCGCTTTATCCTAAAATCATACAAGACATTGAGACAATAGAGTCCAATTGGAGATGTGGGCGACA TAGTTTACAGAGAATCCACTGCCGGAGTGAAACAAGTAAAGGGGTTTACTGTTTACAGTACGACGACCAG AAGATAGTCAGCGGCCTTCGAGACAACACCATCAAGATCTGGGATAAAAGCACACTGGAATGCAAGCGGA TTCTCACGGGCCACACGGGCTCCGTCCTGTGTCTGCAGTACGATGAGAGGGTGATCATCACAGGCTCCTC AGACTCCACCGTCAGAGTGTGGGATGTAAATGCAGGTGAGATGCTAAACACATTGATTCACCACTGTGAA GCCGTTCTGCACCTGCGCTTCAATAATGGCATGATGGTGACCTGCTCCAAAGACCGTTCCATCGCTGTGT GGGATATGGCTTCCCCAACTGACATCACCCTCAGGAGGGTGCTGGTGGGACACCGAGCTGCGGTCAATGT TGTAGACTTTGATGACAAGTACATCGTTTCTGCCTCTGGAGATAGAACCATAAAGGTGTGGAACACAAGT ACCTGTGAATTCGTAAGGACCCTAAATGGGCACAAGCGTGGCATCGCCTGTTTGCAGTACAGAGACAGGC TGGTGGTGAGCGGCTCCTCTGACAACACCATCAGGCTGTGGGACATAGAGTGTGGAGCATGCCTGCGAGT GTTGGAGGGCCATGAGGAGTTGGTACGCTGCATTCGATTTGATAACAAAAGGATAGTGAGCGGAGCCTAT GATGGGAAAATTAAAGTGTGGGATCTTATGGCTGCTTTGGACCCTCGTGCTCCAGCAGGGACTCTCTGTC TGCGGACACTTGTGGAGCATTCTGGAAGAGTTTTCCGTCTCCAGTTTGATGAATTCCAGATTGTCAGTAG TTCACATGATGACACAATTCTCATCTGGGACTTCCTGAATGATCCAGCTGCTCACGCTGAACCGCCCCGC TCCCCTTCTCGGACATACACCTACATCTCCAGATAAATAACCCAACACTGGCCTCATAATTGCCCAGGAC TCGTTAATGTTGCAGTATTTAACAGACCTGCCAAGACCAGGATGAACAACAATCAAACTCCTACCCGGAT TCCCGGACGGATGAGCGAGGAGCAGGGCTTTGAGACTCCTGTTGGGACACAGTCGGTCAGCAGCCGACCA GGACGGCCTGCTCGGCACCGGCTGCCTCAGTGCTGCTATCAGAAGATGTCTTTATCTTGTGTGAATGATT GGAACTTTCAAGCCTCCCTCCCCTTCCCTTCCCCTTCCTCCCTGCACCTGTTTCTCTCCCATTGGGTTCC AGACAAAGATGACTTATAAATATATTTAGTGTTTACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3491843 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3491843 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19435 same embryo
	EMAGE:19436 same embryo
	EMAGE:19438 same embryo
	EMAGE:19437 same embryo
	EMAGE:19434 same embryo
	EurExpress:euxassay_004824 same experiment
	MGI:4823520 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source