EMAGE mouse gene expression spatial database

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EMAGE:19459

Mfsd2a major facilitator superfamily domain containing 2A ( MGI:1923824)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005155. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005155_01	euxassay_005155_02	euxassay_005155_03	euxassay_005155_04

euxassay_005155_05	euxassay_005155_06	euxassay_005155_07	euxassay_005155_08	euxassay_005155_09

euxassay_005155_10	euxassay_005155_11	euxassay_005155_12	euxassay_005155_13	euxassay_005155_14

euxassay_005155_15	euxassay_005155_16	euxassay_005155_17	euxassay_005155_18	euxassay_005155_19

euxassay_005155_20	euxassay_005155_21	euxassay_005155_22	euxassay_005155_23	euxassay_005155_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19459_wholemount_strong.wlz

19459_wholemount_moderate.wlz

19459_wholemount_weak.wlz

19459_wholemount_possible.wlz

19459_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19459_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

moderate

regional

moderate expression: see section 05 06 21 24 weak expression: see section 22

foot

moderate

regional

moderate expression: see section 06 07 21

submandibular gland primordium

moderate

regional

moderate expression: see section 15 18 weak expression: see section 06 07 08 16 17

brain

strong

spotted

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 21 22 24 weak expression: see section 06 07 08 09 10

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 19 20 22 weak expression: see section 06

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 06 19 20 22

spinal cord

strong

spotted

strong expression: see section 07 08 09 10 11 12 13

pharyngo-tympanic tube

weak

regional

weak expression: see section 01 02 03 22 24

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 15 19

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 14 15

lower jaw incisor

moderate

regional

moderate expression: see section 13 weak expression: see section 11 12

lower jaw molar

moderate

regional

moderate expression: see section 08 19

upper jaw incisor

moderate

regional

moderate expression: see section 13 17 weak expression: see section 12

upper jaw molar

moderate

regional

moderate expression: see section 08 19 not examined expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8613
Entity Detected:	Mfsd2a, major facilitator superfamily domain containing 2A ( MGI:1923824)
Sequence:	sense strand is shown >T8613 GTTCCTGGTTTGCTAAGTGCTAGCGAACGTAGTAGTGGCTGGGGCTGCGCCTAGGATCGGAGCCACAGGC GAGTGCCGGCCGGCAGAACACAAGAGCATCCCGGCGTCCCGGTCCCACGCGGCGTGGCCCGCGGGTCATG GCCAAAGGAGAAGGCGCCGAGAGCGGTTCCGCGGCGGGGCTGCTCCCCACCAGCATCCTCCAAGCCAGTG AACGGCCGGTCCAGGTGAAGAAGGAACCAAAAAAGAAGCAGCAACTGTCCATTTGCAACAAGCTTTGCTA TGCAGTTGGAGGGGCCCCGTACCAGTTGACCGGCTGCGCACTGGGATTCTTCCTGCATATCTACCTATTG GATGTGGCTAAGGTGGAACCACTTCCTGCTTCCATTATCCTTTTTGTGGGCCGAGCCTGGGATGCCTTCA CTGACCCTCTGGTGGGCTTCTGCATTAGCAAGTCCTCCTGGACCCGCCTGGGCCGCCTCATGCCCTGGAT CATCTTCTCCACTCCCCTGGCCATCATTGCTTACTTCCTCATCTGGTTTGTGCCTGACTTCCCATCAGGG ACGGAAAGTTCACACGGCTTCCTTTGGTACCTGCTTTTCTATTGCCTCTTTGAGACACTGGTCACGTGCT TTCATGTTCCCTACTCAGCGCTCACCATGTTCATCAGCACGGAGCAGAGTGAGCGTGACTCAGCCACGGC ATACAGAATGACTGTGGAGGTGCTGGGCACAGTGATAGGCACAGCGATTCAAGGACAAATTGTGGGCCAA GCCAAGGCACCTTGTCTCCAGGACCAGAATGGCTCTGTGGTGGTCTCAGAAGTTGCCAATCGCACCCAGA GTACTGCCTCCCTCAAAGACACGCAAAATGCTTACCTGCTGGCAGCAGGGATCATCGCCTCCATCTACGT CCTCTGTGCCTTCATTCTGATCCTAGGCGTGCGGGAGCAGAGAGAACTCTACGAGTCCCAGCAGGCTGAG TCAATGCCCTTCTTTCAGGGCCTCCGGCTGGTCATGGGTCATGGCCCCTATGTCAAGCTCATTGCCGGCT TCCTTTTTACCTCCCTGGCTTTCATGCTGGTGGAGGGTAACTTTGCCTTGTTCTGCACCTATACCTTGGA CTTCCGAAATGAGTTCCAGAACCTCCTCCTGGCCATCATGCTCTCGGCCACATTCACCATCCCTATCTGG CAGTGGTTCCTAACCCGGTTTGGCAAGAAGACAGCTGTATACATCGGGATCTCTTCTGCAGTTCCTTTTC TCATCTTGGTGGCCCTCATGGAGCGTAATCTAATCGTCACTTACGTGGTGGCCGTAGCAGCTGGCGTCAG TGTAGCAGCTGCCTTCCTACTACCATGGTCCATGCTGCCTGACGTTATCGATGACTTCCACCTGAAACAC CCTCACTCCCCTGGCACCGAGCCCATATTCTTCTCCTTCTATGTCTTCTTCACCAAGTTTGCCTCTGGAG TCTCACTGGGTGTCTCTACCCTCAGTCTCGACTTTGCCAACTACCAGAGGCAGGGATGCTCCCAGCCAGA ACAGGTCAAGTTTACACTGAAGATGCTGGTGACCATGGCTCCTATCATCCTCATCTTGCTGGGCCTGCTG CTCTTCAAGCTCTACCCCATTGATGAGGAGAAGCGGCGACAGAATAAGAAAGCTCTGCAGGCTCTACGAG AAGAAGCCAGCAGCTCAGGTTGCTCGGATACAGACTCCACAGAGCTGGCCAGTATTCTCTAGGACTTGCC AGGGTGCCTGAAGCCACCACACAAAGGACCTGGGGCACCAGGTAGCATTAAGCACGGGATCTCTTTGCCC TTGCACTGAGCAGCTGTTCTTCCTATGTCAAGACAGGGACCAAGGAGTGGAGGGTGAGCGGACCAGGACA TCTGTGCTCACTGCAAGGCTGACCGCTCTGTCACCTCCTCACTGCCTGCCCACTGGGCCAAGCCTTAGGG CTGCTACTGTGAATATGCCAAGGACTGACTGGGCCTAGCTCGGAACACTAATGTAGAAACGTTTTATACA GAGACTAATTAATAACTTAATGACTATGTATATAGTGATGTGTGTATGTATGTGTCTATGAGGTATTAAT GTTATTAATTTTCATAAAAACTGGAAACACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5717701 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5717701 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19461 same embryo
	EMAGE:19463 same embryo
	EMAGE:19458 same embryo
	EMAGE:19460 same embryo
	EMAGE:19462 same embryo
	EurExpress:euxassay_005155 same experiment
	MGI:4826182 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source