EMAGE mouse gene expression spatial database

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EMAGE:19460

Fsd1 fibronectin type 3 and SPRY domain-containing protein ( MGI:1934858)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005186. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005186_01	euxassay_005186_02	euxassay_005186_03	euxassay_005186_04

euxassay_005186_05	euxassay_005186_06	euxassay_005186_07	euxassay_005186_08	euxassay_005186_09

euxassay_005186_10	euxassay_005186_11	euxassay_005186_12	euxassay_005186_13	euxassay_005186_14

euxassay_005186_15	euxassay_005186_16	euxassay_005186_17	euxassay_005186_18	euxassay_005186_19

euxassay_005186_20	euxassay_005186_21	euxassay_005186_22	euxassay_005186_23	euxassay_005186_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19460_wholemount_strong.wlz

19460_wholemount_moderate.wlz

19460_wholemount_weak.wlz

19460_wholemount_possible.wlz

19460_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19460_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 05 moderate expression: see section 06 07 18 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 moderate expression: see section 16 17 not examined expression: see section 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 moderate expression: see section 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 moderate expression: see section 07 16 17 18 not examined expression: see section 19

trigeminal v nerve

strong

regional

strong expression: see section 09 moderate expression: see section 17

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 13 14

cervical ganglion

moderate

regional

moderate expression: see section 07 14 15

thoracic ganglion

weak

regional

weak expression: see section 08 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 12 13 14 15

retina

moderate

regional

moderate expression: see section 02 03 04 05 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 15 17 18 weak expression: see section 11 12 14 16 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9620
Entity Detected:	Fsd1, fibronectin type 3 and SPRY domain-containing protein ( MGI:1934858)
Sequence:	sense strand is shown >T9620 GCGGCGGCGGAGGCGGCGAGGGCACGGAAGGCTAGTTGGCTGCTGTCTGCGTCCAAGGAACTTTAAGGGT TTGGAGAACCAGCTGAAGGGGACCCGCGGCCAGGCCAAGGCAACAGGGGGCTGCAACTATGGAAGACCAG AGGGAGGCGCTAAGAAAAATCATCACTACCCTGGCCATGAAGAATGAGGAGACGCAGACCTTCATCTACT CCCTGAAGCAGATGTTGCTGAATGTAGAGGCAAACTCAGCCAAAGTCCAGGAGGATCTGGAAGCCGAGTT CCAGTCCCTCACGTCGGTTCTGGAGGAGCTGAAAGAGTCCATGCTGATGAAGATCAAACAGGACAGGGCC AGCCGCACCTACGAGCTGCAGAACCAGCTAGCTGCCTGCACACGAGCCCTGGAGAGCTCCGAGGAGCTCC TGGAAACAGCCAACCAGACCCTGCAGGCTTCAGACAGCGAGGACTTCAGTCAGGCCGCCAAGGAAATCAA AGATGGCATAACTATGGCCCCTGCCTTCCGACTATCGCTCAAAGCCAAGGTGAGCGACAATATGAGTCAC CTCATGGTGGACTTCGCCCAGGAAAGGCAGATGCTACAGGCCCTCAAGTTCCTACCTGTGCCCAGCGCCC CCACCATCGACTTGGCAGAGTCCCTCGTGTCAGATAACTGTGTGACGCTGGTATGGCACATGCCAGATGA GGACAGCAAGATCGACCACTATGTGCTGGAGTACCGCAAGACCAATTTCGAGGGCCCACCCCGCCTCAAG GAGGACCACCCCTGGATGGTTGTCGAGGGCATCCGGCAGACCGAGCACACCTTGACAGGTCTCAAATTTG ACATGAAATACATGAACATTCGTGTGAAAGCCTGTAACAAGGCGGTGGCTGGTGAATTCTCCGAGCCGGT GACCTTAGAGACACCAGCATTCATGTTCCGCCTGGACGGGTCCACATCTCACCAGAACCTGCGAGTGGAA GATCTCTCTGCCGAGTGGGACGCCATGGGCGGGAAGGTCCAAGATATCAAGGCTCGAGAGAAAGAGGGCA AGGGACGGACCGCATCCCCTGTAAACTCTCCAGCCAGAGGGACTCCATCTCCCAAGAGGATGTCCTCAGG TCGTGGGGGCCGGGACCGGTTCACAGCTGAGTCTTACACGGTGCTGGGGGACACACTGATTGATGGTGGC GAGCACTACTGGGAGGTGCGCTTCGAACCAGACAGCAAGGCCTTTGGCTTGGGTGTGGCCTACCGCAGCC TGGGCCGATTCGAGCAGCTGGGCAAGACGGCTGCATCATGGTGCCTGCATGCCAACAACTGGCTGCAGGC CAGCTTCACCGCCAAGCACGCCAACAAGGTCAAAGTGCTGGACAGCCCTGTGCCTGACTGCCTGGGTGTG CACTGCGACTTCCACCAAGGCCTCCTATCTTTCTACAATGCCCGCACCAAACAGCTCCTGCACACCTTCA AGGCCAAGTTCACGCAGCCTCTGCTTCCAGCCTTCACCGTATGGTGTGGAAGCTTCCAGGTGACAACAGG CCTGCAGGTGCCCAGTGCCGTGCGCTGCCTGCAGAAGCGGGGCAGTGCCACTAGCAGTTCCAATACCAGC CTCACCTAGGCCAGGCCATCTACCAGCTGGGGTGCTTCAGCCCAGCTTCTTCTTTCTGCTGCTTGGAGCC TTAACTCTGGAAGGGAGGGTCAGGGTTCCCTCCCCACCTCACTCTATAATAAAGATAAAACACTGGGCAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6532001 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6532001 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19461 same embryo
	EMAGE:19459 same embryo
	EMAGE:19463 same embryo
	EMAGE:19458 same embryo
	EMAGE:19462 same embryo
	EurExpress:euxassay_005186 same experiment
	MGI:4824936 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source