EMAGE mouse gene expression spatial database

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EMAGE:19463

Slc15a3 solute carrier family 15, member 3 ( MGI:1929691)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005166. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005166_01	euxassay_005166_02	euxassay_005166_03	euxassay_005166_04

euxassay_005166_05	euxassay_005166_06	euxassay_005166_07	euxassay_005166_08	euxassay_005166_09

euxassay_005166_10	euxassay_005166_11	euxassay_005166_12	euxassay_005166_13	euxassay_005166_14

euxassay_005166_15	euxassay_005166_16	euxassay_005166_17	euxassay_005166_18	euxassay_005166_19

euxassay_005166_20	euxassay_005166_21	euxassay_005166_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19463_wholemount_strong.wlz

19463_wholemount_moderate.wlz

19463_wholemount_weak.wlz

19463_wholemount_possible.wlz

19463_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19463_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8614
Entity Detected:	Slc15a3, solute carrier family 15, member 3 ( MGI:1929691)
Sequence:	sense strand is shown >T8614 GGAGATAGAAACTAGGTAGCAAGGAGAGGGACCTGAGGGAAAAAGAGAGAGAGAACGACACGGGGTGAGA AAAGAGGGCGATCAGGGTTAGGGGAGAGGAGGGGAGGACAGAGGAGAGAGTCTGGGTCACGGAGACAGGA AGGCCAGGATGTCTGCCCCTCGCGCGGAAGAGCAACCAAGCAGGTCCGGAGAGCGACAACCGCTGGTGGC CCGAGGCCCGCGGGGACCACGACGGTGGAGACGGACAGCTGCTGCTGCGGTGCTGCTAGTGCAGATGCTG GAACGCGCTGCCTTCTTTGGTGTCACCTCCAACCTCGTGCTCTACCTCAACAGCTTGAACTTCAATTGGG ACGGCCAACAGGCATCACGGGCCACACTCCTCTTCCTAGGCGCCTCCTACCTGCTGGCTCCAGTGGGGGG CTGGCTGGCAGACGTGTACCTGGGACGCTTCCTCACTATCTCGCTCAGCCTGCTGCTCTATCTGGCTGCC TCTGGCTTGTTGCTCACCACCATCACCAATGATGGCCGCAGATCCTTCTGTGGAGAGATGCCCGAGTTGC CACTGGAACCTGCCTGCCCATCCTCAAGCTGCCAAGGCTCCTGGTCCAGCCCCTACTGCGCGACCACCCT CTACTTGGTGTTGCTGCTCCTGGCCCTGGCTGCCAGCTCCGTCAGGAGCACCCTCACCTCGTTCGGGGCT GACCAGGTGATGGATCTCGGGCGCGATGCCACCCGCCGCTTCTTCAACTGGTTCTATTGGAGCATCAACC TGGGTGCCATATTGTCCCTGTTGGTGGTGGCTTTCATCGAGCAGAACATCAGCTTCCTATGGGGCTACAG CATCATCGTGGGCCTCGTGGGCCTGGCATTCTTCATTTTTCTCTTTGCCACACCAGTCTTCATCACCAAG CCCCCAACAGGCAGCCAAGTGTCATCTATGCTGAAGCTTGCGTTCCAAAACTGCTGTCCCTGCCGGAGGT CCTCTTCCAGGGACTCTGAAAGTGCCCACCTGTTGCCTGACCAGAGGTCTAACCAGCCTGGACCTTCTCC ACAAGAAGATATGGCCAACTTCCAGGTGCTGGTGAAGATCCTGCCTGTGATGGTGACCCTTGTGCCTTAT TGGATGGTATATTTCCAGATGCAGTCCACCTATGTCCTCCAAGGTCTCCACCTCCATATCCCCAACATCT TCAGGACCAACCCTAACATCTCTTTGTTGCTGAGATCAGATAGCAGTAACTACAGGATCCCAGAAGCCTG GCTCCTACTGGCCAATGTTGCGGTGATCCTGATTCTGATCCCTGTCAAGGATCACTTGATTGATCCTCTG CTGCTGCGGTGCAAGCTGCTGCCCTCATCTCTGCAGAAAATGGCCCTGGGCATGTTCTTTGGGTTCACCT CCATCATTGTGGCAGGAGTCCTGGAGAAAGAACGCTTACAGTACATTGCTGCCAACCAGACAGTGCCTCA GCTGATTGGGAAGGACTTATACTATGCAGCGCCACTGTCTATCTGGTGGCAGATCCCCCAGTACCTGCTC ATCGGGGTCAGTGAGATCTTTGCCAGCATCCCAGGTCTGGAGTTCGCTTACTCAGAAGCCCCACGCTCCA TGCAGGGTGCAATTATGGGCATCTTCTTCTGCCTTTCCGGGGTGGGCTCACTGCTAGGCTCTGGCTTGGT GGCCCTACTGTCTTTTCCTGGGGGCTGGATGTACTGCCCCAAGGACTTTGGGAACATCAACAACTGTCAG ATGGACCGTTACTTCTTCCTGCTGGCCGGCATTGAGGCTGTCACAGCCGTCCTGTTTCTCTGGATTGCTG GTCGCTATGAGAGGACTCGCCAGGACCCAGCCTCCCAGCGCTCGTCCAGCAGGGTCAGAGGCTAATGGTC TCCACCCACCCTGACAGAGAGTGTCACAGCAGTACCAGCTATGCCCATTCTTTTCTCATTTATTCTGTAC CCAACAGTAGAATGAAGTGGTTCCCATAAATAAGGGGCATGCGCCCTCCCTCTTGAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5717926 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5717926 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19461 same embryo
	EMAGE:19459 same embryo
	EMAGE:19458 same embryo
	EMAGE:19460 same embryo
	EMAGE:19462 same embryo
	EurExpress:euxassay_005166 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source