EMAGE mouse gene expression spatial database

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EMAGE:19519

Kpna3 karyopherin (importin) alpha 3 ( MGI:1100863)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004924. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004924_01	euxassay_004924_02	euxassay_004924_03	euxassay_004924_04

euxassay_004924_05	euxassay_004924_06	euxassay_004924_07	euxassay_004924_08	euxassay_004924_09

euxassay_004924_10	euxassay_004924_11	euxassay_004924_12	euxassay_004924_13	euxassay_004924_14

euxassay_004924_15	euxassay_004924_16	euxassay_004924_17	euxassay_004924_18	euxassay_004924_19

euxassay_004924_20	euxassay_004924_21	euxassay_004924_22	euxassay_004924_23	euxassay_004924_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19519_wholemount_strong.wlz

19519_wholemount_moderate.wlz

19519_wholemount_weak.wlz

19519_wholemount_possible.wlz

19519_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19519_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6914
Entity Detected:	Kpna3, karyopherin (importin) alpha 3 ( MGI:1100863)
Sequence:	sense strand is shown >T6914 CGGACGCGTGGGCGCCGCAGGAGCAGACGCCTCCGGAGCCGCGCGCAGCCATGGCCGAGAACCCCGGCTT GGAGAACCACCGCATCAAGAGCTTCAAGAACAAGGGCCGCGATGTGGAGACGATGCGAAGACACAGAAAT GAAGTGACAGTTGAACTCCGGAAGAACAAAAGAGACGAACACTTACTGAAAAAGAGAAATGTTCCCCAAG AAGAAAGCTTAGAAGATTCAGATGTTGATGCTGATTTTAAAGCACAAAATGTAACACTAGAAGCTATATT ACAGAATGCCACAAGTGATAATCCTGTAGTCCAGTTGAGTGCTGTGCAGGCAGCAAGGAAACTATTATCC AGTGACAGAAATCCACCAATTGATGACCTAATAAAATCTGGGATTTTACCAATTCTAGTCAAATGTCTAG AAAGAGATGATAATCCTTCATTACAGTTTGAAGCTGCTTGGGCACTAACTAATATAGCATCGGGAACTTC TGCACAGACTCAAGCTGTTGTACAGTCTAATGCAGTACCACTTTTTCTGCGACTCCTCCATTCCCCACAT CAGAATGTTTGTGAACAAGCGGTGTGGGCTTTGGGAAACATTATAGGTGATGGTCCTCAATGTAGAGATT ATGTCATATCACTGGGAGTTGTCAAACCTCTTCTGTCCTTCATCAATCCCTCCATTCCCATCACCTTCCT TCGGAACGTCACATGGGTCATTGTCAATCTCTGCAGGAATAAAGACCCCCCACCGCCTATGGAGACAGTT CAGGAGATTTTGCCAGCTTTGTGTGTGCTCATCTACCATACGGACATAAATATTCTTGTAGACACTGTTT GGGCGCTGTCATACTTGACAGACGGGGGAAATGAGCAAATACAGATGGTTATTGATTCAGGGGTTGTACC CTTCCTCGTACCCCTTCTGAGCCACCAGGAAGTCAAAGTTCAAACTGCAGCACTCAGAGCGGTTGGGAAC ATAGTGACTGGCACTGATGAGCAGACCCAGGTGGTTCTCAACTGTGATGTCCTGTCCCACTTCCCAAACC TCTTGTCACACCCAAAAGAGAAAATAAATAAGGAAGCAGTATGGTTCCTTTCCAATATAACAGCAGGCAA TCAGCAGCAAGTTCAAGCTGTAATAGATGCTGGGTTAATCCCTATGATTATTCATCAGCTCGCTAAGGGG GACTTTGGAACACAAAAGGAAGCTGCTTGGGCAATTAGCAACTTAACGATAAGTGGCAGAAAAGATCAAG TTGAATACCTTGTACAGCAGAACGTAATACCACCATTCTGTAACTTACTGTCAGTAAAAGATTCTCAAGT GGTGCAGGTGGTTCTAGATGGTCTTAAAAACATCCTGATAATGGCTGGTGATGAAGCAAGCACAATAGCT GAGATCATAGAAGAGTGTGGAGGTTTGGAGAAAATTGAAGTTTTGCAGCAACATGAAAATGAAGACATAT ATAAATTAGCATTTGAAATCATAGATCAATATTTCTCTGGTGATGATATTGATGAAGATCCTAGCCTCAT TCCAGAAGCAACACAAGGAGGTACTTACAATTTTGACCCAACAGCCAACCTTCAAACAAAAGAATTTAAT TTCTAAGGTCAATTAAGTGCAGCATCTTTCATTCAATATGAAACACCACCAGATGGCCACCAAGCGATAA GACAGCAAGCAACAGAAGGCTCCCAGCACTCTTGCCTCTTCATTTTGATGCTTCTAAAGCAAGCCATGTC TCAGTCACTTTGCAGTTGCCAAAAGTCACTCTCACATGGACTGTAAATGCATATGCATGATTTCCTAAAC TGTTTTAGAATTCTCCTTAACCATCTCAACTCCCCTATATTTTCCATGTTCCCTGGTGCCACATGCTGAC AACTGCAGTCTCCAGTTAGAATATTCCATAGTGGTGGTATATCAGCTGCCCACTGATACGCCTTTGGAAA AAGGTGCGCTGTGGATCAAGACACTTTGGTATGATGTATATTCATGTTGGAAGACTACAGAGGTGCAGTG TGATCTGAGCCTCCATCATTGTCCTCCACAAACATATTTTCATATTCTTATGTGGAAGAATTAAAAGTAC AAGCCAAATGATTTTCATTGGTGAAACTGACACAAAAGTAACTTAAAAACAAGAAACTTGGTTGTTGATT AAAAAGATAAGTTTTTTAAAACTGTACTTCACCTACCATTAATTGATGTGTTTATTATCTGCCTCAGAAG CCAGGGTTGGAGGAAGAGCTTTAGATATCAATGGTAATGCTTTCGCCATTATACCTAATTTTTTGAGAAC AGCAAATCCTATTTGACCACTCACTTCAGCCTGTGTGTTCCTGCTGTTTTGAATAATCAAATGCTGTGCA TGGTCTCTACCTGAGCTGCAACCTGTTACAGACTTGAACTTCTGTTAAGTTGAAAGCAAGAGTCCTTGAC TACAAAAATACACAAATAAATAAGTCAAAACAAGAATGGAAAAGGTTTAAATACTGATTGGTAAGGTTTT AAGACAAAGTCTTGCTTTCAGCTTCCAGGGGTTAATTTTATTTTTGTTTTAATTTGATAATTGGATATGG TTGATTTATATTGGGTTTAAACTGTGGAGCTTTCATGTTTACTGTAATTTAGTCTTAAAATATTTTTTAC TTAGTAACCAGTACTTTTGATAATGTGGTGGCAACAAACCAGCAACTATCTAGAAGTGTTACAAGACTTC ATTCTTGAGTATTGGAGTTCATTCAGATCTCACTCAAAGCATCTGCACACATTAAAAGATAAACCAAGGA TCTGTGTAGAGGAATGGTCTGCAGTTATTTAACTCAGCCTGATTTGCAGTGATCTTAAGCTCTGCAAAAT CTGGCCTTACTATGTAAAGTCATTGCTTATGGCAAATGGTCTGACCCAGTTATTAATGAATGAATAGGGA TTTGAAATTTTTTAAACTAAATAATTTGTGCTGTCACAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3979788 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3979788 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19521 same embryo
	EMAGE:19520 same embryo
	EMAGE:19517 same embryo
	EMAGE:19518 same embryo
	EurExpress:euxassay_004924 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source