EMAGE mouse gene expression spatial database

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EMAGE:19520

Card14 caspase recruitment domain family, member 14 ( MGI:2386258)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004918. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004918_01	euxassay_004918_02	euxassay_004918_03	euxassay_004918_04

euxassay_004918_05	euxassay_004918_06	euxassay_004918_07	euxassay_004918_08	euxassay_004918_09

euxassay_004918_10	euxassay_004918_11	euxassay_004918_12	euxassay_004918_13	euxassay_004918_14

euxassay_004918_15	euxassay_004918_16	euxassay_004918_17	euxassay_004918_18	euxassay_004918_19

euxassay_004918_20	euxassay_004918_21	euxassay_004918_22	euxassay_004918_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19520_wholemount_strong.wlz

19520_wholemount_moderate.wlz

19520_wholemount_weak.wlz

19520_wholemount_possible.wlz

19520_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19520_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 05 06 07 18 19 20

naris

moderate

regional

moderate expression: see section 09 10 14 15

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 10 13 14

tongue epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

rectum

moderate

regional

moderate expression: see section 13

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6911
Entity Detected:	Card14, caspase recruitment domain family, member 14 ( MGI:2386258)
Sequence:	sense strand is shown >T6911 CCCACCACAGCCTGTAGAGAAAAAGCCATTGGCGGTTTGCACCGGCCAGATTGGAGACACCAGACTCCAG CCAGCTACAAGGAGGCCCCGAAGAAGCAGCTCTGGGCACCTCTGATCCTGACCGGTTTTTATTGTCATCT TGGGTTAATGAAGTCCTGGGCCATGAGCAACAAATGACTAATGTGGTTTACTGACACCAGCTGAAGCTGC CTGCCTGCCAGAGCAGTGTGGAGCCAAGAGGGCTGGAGCAGAAAACTCCCTGGCCTTGGAAGTCCAAGCG CAGTCTTTCATCGAACCCCTATCCCAGCGTCTCAGAGTTCAGGTATGGCAGAACTGTGCCGCATGGATTC CACACTGACTGCTCTGGACGAGGAGATGCTCTGGGATATGCTGGAGAGTCACCGATGCAGGATTGTGCAG AGCATCTGCCCTAGCCGGCTCACCCCCTACCTGCGCCAGGCCAAGGTGCTGGGCCAGCTGGACGAGGAGG AGATCCTGCATAGCTCCCGTTTCACCAACAGTGCCATGAGAGTTGGGCACTTGCTGGATCTGCTAAAGGC CCGAGGGAAGAATGGAGCCATTGCCTTTCTGGAAAGCCTGAAGTTCCACAACCCTGATGTCTACACCCTG GTCACTGGGCTGCAGTCTGACATTGACTTCAGCACTTTCAGCGGTCTCATGGAGACATCCAAGCTGACCG AGTGTCTGGCTGGGGCCATCAGCAGCCTGCAGGAGGAGCTGGCCCAGGAGAAGGCACAGAAGGAGGTTCT GCTCCGGAGATGCCAGCAGCTGAAGGAGCGCCTGGGCTTGGCTGAGGCTCACGCAGAAGGTCTGCGCCAG CTGGAGGTCGACCACAGCCGCATGAAACGTGAGGTCAGCACCCACTTCCACGAGGTCCTGAAACTGAAGG ACGAGATGCTGAACCTGTCACTGCACTACAGCAACGCACTCAGAGAGAAGGAGCTGGCTGCCACACGCTG CCACAGCCTTCAGGAAGAGCTCTACCTGGTGAAGCAGGAACTCCAGCGAGCAAGCCTTGTATCGTCATGT GAAAGAGAATCTCGAGAGAGGTCCCTGAAGATGGCCAGCAACCTGGAGCCTCAGGGAGAAGAACTAAATC GGCTTAAGGAGGAGAATGAGAAACTCCGCTCCATGACCTTCAGCCTGGTGGAGAAGGACATTCTGGAACA GAGTCTGGATGAGGCCAGGGAGAGCAAGCAAGAGCTGGTGGACCGCATCCACTCACTGCGGGAGAGAGCA GTGGCCGCGGAGAGGCAGCAGAAGCAGTACTGGGAGGAGAAGGAACAGACCCTACTCCAGTTCCGGAAGA CACAGGTGGACTGTGAACTATACAAAGAAAAGATGACCATGCTTCAGGGCCAGGTGGCTGAGCTGCAGAA GGAACGTGACCAGGCATACACAGCAAGGGACAGGGCCCAGATGGAGATTTCTCAGCGCCTGGTGGAGAAG GATGCCCTTCGCAGGAGAGTGTTCGAGCTGACGGAGCAGGTCTGTGAGCTGCGTACTCAGCTGCGCAGGC TGCAGGCGGAGGCCCCAGGAGGACCCAAGCAGGAAGCCGGAGCCAGGGAACTCTGCCTCCGGGGGAAGCA GAGGCTTGTGCGGATGCATGCTGTGTGCCCACCAGATGACAGTGACTGCAGCCTCCTCAGCTCCACAGAG TCCCGGTTGTGGTGGGACCTGAATTCCACATCCAGCCGGGAGCAGATGGACAGCTTCCGCTCCAGCAGCC CCATGCCTCCCAGCCAGCAGTCCCTGTACAAGCGGGTGGCAGAGGACTTCCTGGAAGACCCCGAGTCTCT AAGCTTCCCAGAAGTCCTTGAGATGCGCCTTCAAGGGGCTACGGTGGACGACACAGACACAGACCTGGAG TTTGAGATGATTGATGGAGCAGACCTCTCTCAGACAGAAGACAGCCTGCAGGGGTCTTCCCGGAGCCTGA ATGTCTCAGAAAGCAGTGTCCCCGTGAGGAGGAGGCCAGCCCGCAAGATCCTAAGCCAGGTCACGGTGCT GGCGTTCCAAGGGGACGCGTTGCTGGAACAGATTGGTGTCATCGGTGGGAACCTCACTGGGATCTTCATT CACCGTGTCACCCCCGGCTCTGCAGCAGATGAGATGGCCCTGCGCCCTGGTACCCAGATCATGATGGTGG ACTATAAACCTACCAAGCCATCATTAAGGGCCACTCTGGAGAATACAACGCTGGAGCAGGCTGTGGGCCT TCTCAGGAGGGTGAATGGCTCCTGCTACTTGTCTGTGAAGATCAACACTGAAGGTTATAAGAACCTCATC CAGGACCTAGATGCCAAAGTGGTAACGTCTGGGGATTCCTTCTACATCCGTGTCAACCTGGCCATGCAGA GGGGAGGGGACGGAGAACTGCAAACTCACTGCAATGACATCCTGCATGTCACTGACACCATGTTCCAAGG CCGCAGCTGCTGGCATGCCCACCATGTGAACCCCTACACCATGAAAGACATGGAGCCTGGCACCATCCCC AACTATTCACAGGCTCAGCAGCAGCTTCTGGCCCTCATCCAGGACATGACTCAACGGTGCACAGTCCCCC GCAAGCCTCCTGGAGGACCACAGAAGTTAGTACGAATTGTCAGTGTGGACAAAGCCGCTGTCAGTCCTCT GACTTCATCCTTTGACCAAAGCCAGTGGGATTCTGGCAAGGAGGAAGGTGGTCCTAGCGTGTGCTTCTGG TCAGAGAGCTGCTTCACCCTGGCACCGTACACCCTGGTGCACCCCCACAGGCCTGCCCGGCCCCGGCCTG TGCTGTTTGTGCCTAGATTAGTTGGAAGGATCCTGGGTAAAAAGCTGTGTCTCCTCCAAGGTTTTAAGCA ATGTTCAGCAGAGTACTTGAGCCAGGAGGAGTATGCCACCTGGAGTCAGAGAGGGGACATCATCCAGGAG GGAGAGTCAATAGGTGACCACCACTGGATCACCCGGCATGCTGTGGAGTCCCTCATGAATATGAGCACCC ACGCCCTGCTGGATGTGCGGCTGGACAGCGTCCGAGTCCTACATAGGATGGACATGTTTCCCATTATCAT CCATGTCTCTGTCAATGAGAAGACAGCAAAGAAACTCAGGAAGGGCCTGCATCGGCTTGGCAGCTCGGAG GAGCAGTTCCTGGAGGTGGCCCGGCAGGAGGAGGGGGAGCTGGACAGAGTGCCGTGTCTGTACAGCAGCC TGGCCCCCGACAGCTGGAGTGATCTGGACAGCCTGCTCAGCTGTGTGCGCCTGGCCATTGCAGATGAGCA GAAGAAGGTGGTGTGGACAGAGAGCCCCTGCTGAAGCCCAGACCTGGCTTGGGACTGTGGGGACCTCTGT GTGTCTGTCAATGCTGTCAGCAAACAACCCCACTTCCTTCTCTGGTCGGGGTCTGACCTCAGGTGCCCCC AGAAATCAAGGTGTAAATGACGAAGCCACCTTCACACAGCATGGCCTGGGCTGATGGAGGACCGGAAAAT ATTCTGCCGTGTGGGGGTACTCCCTGAGACCTGCTCAGAGCCAGTGCCCCAACCTCCTGTGACAACAGGA ATAGCTTTGTGGGTTCTGGCCCTTCCTTTTATGTTTCTGATGTCAAAGGAACCTTGCAAGCTCGCTGGAG CAGTCCTTCCTGCCCAGGGCGGGGGCCTGACTCTGGAGCACCGTGGGCACAGCTGACTCAGCTGCTAACA GCCAAGTGCTGCCACAGACATCTGCAAATGAAGGTGCTGTAGTGCACAGACTTGAGTGGCTCTTTGACTC ACTGCCTCTGTGCCTAACCCCAAACCCCAGGTTGCTTTGGTGCATATCCCAGAGCCAACTGACCCTTCAT GAGCAAAGAACTCTCTCTCAAAACTGACATTTCCCCCAGTCCTTCCCAGGCTGCATTGCGCCACACTTGG GTGAAGCCCCCATCTAGCCATAAACTGAGTCATTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:3979883 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3979883 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19521 same embryo
	EMAGE:19519 same embryo
	EMAGE:19517 same embryo
	EMAGE:19518 same embryo
	EurExpress:euxassay_004918 same experiment
	MGI:4823636 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source