EMAGE mouse gene expression spatial database

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EMAGE:19529

Hyou1 hypoxia up-regulated 1 ( MGI:108030)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004962. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004962_01	euxassay_004962_02	euxassay_004962_03	euxassay_004962_04

euxassay_004962_05	euxassay_004962_06	euxassay_004962_07	euxassay_004962_08	euxassay_004962_09

euxassay_004962_10	euxassay_004962_11	euxassay_004962_12	euxassay_004962_13	euxassay_004962_14

euxassay_004962_15	euxassay_004962_16	euxassay_004962_17	euxassay_004962_18	euxassay_004962_19

euxassay_004962_20	euxassay_004962_21	euxassay_004962_22	euxassay_004962_23	euxassay_004962_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19529_wholemount_strong.wlz

19529_wholemount_moderate.wlz

19529_wholemount_weak.wlz

19529_wholemount_possible.wlz

19529_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19529_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7979
Entity Detected:	Hyou1, hypoxia up-regulated 1 ( MGI:108030)
Sequence:	sense strand is shown >T7979 GCCGCTGAGAGGGTCACAATGGCAGCCACAGTAAGAAGGCAGAGGCCAAGGAGGCTACTCTGTTGGGCCT TGGTGGCTGTCCTCTTGGCAGACCTGTTGGCACTGAGCGACACATTGGCTGTGATGTCTGTAGACCTGGG CAGTGAATCCATGAAGGTGGCCATTGTCAAGCCTGGAGTGCCCATGGAGATTGTATTGAACAAGGAATCT CGGAGGAAAACTCCAGTGACTGTGACCTTGAAAGAAAATGAAAGGTTTTTAGGTGATAGTGCAGCCGGCA TGGCCATCAAGAACCCAAAGGCTACGCTCCGTTATTTCCAGCACCTCCTTGGAAAACAGGCGGATAACCC TCATGTGGCCCTTTACCGGTCCCGTTTCCCAGAACATGAGCTAATTGTTGACCCACAGAGGCAGACTGTG CGCTTCCTGATCAGTCCGCAGCTGCAGTTCTCTCCCGAGGAGGTACTGGGCATGGTTCTGAACTACTCCC GTTCCTTGGCTGAAGATTTTGCTGAACAACCCATTAAGGATGCAGTGATCACCGTGCCAGCCTTTTTCAA CCAGGCTGAGCGCCGAGCTGTGCTGCAGGCTGCTCGGATGGCTGGCCTCAAGGTGCTGCAGCTCATCAAT GACAACACTGCCACAGCCCTCAGCTACGGTGTCTTCCGCCGGAAAGATATCAATTCCACTGCACAGGACG TCATGTTCTATGACATGGGCTCGGGCAGCACTGTGTGCACCATCGTCACCTACCAGACAGTGAAGACTAA GGAGGCTGGGATGCAACCACAGCTGCAGATCCGGGGCGTGGGATTTGACCGCACCCTGGGTGGCCTGGAG ATGGAGCTTCGGCTTCGAGAACACCTGGCTAAGCTCTTCAATGAGCAGCGCAAGGGCCAGAAAGCCAAGG ATGTTCGGGAAAACCCCCGGGCCATGGCCAAACTGCTTCGGGAAGCCAACCGGCTTAAAACCGTCCTGAG TGCCAACGCTGATCACATGGCACAGATTGAGGGCTTGATGGATGATGTGGACTTCAAGGCCAAAGTAACT CGGGTGGAATTCGAGGAGCTGTGCGCAGATTTGTTTGACCGTGTGCCTGGACCTGTGCAGCAGGCCTTGC AGAGTGCAGAGATGAGCTTGGATCAAATTGAGCAGGTGATCCTGGTGGGCGGGGCCACTCGTGTTCCCAA AGTTCAAGAAGTGCTGCTCAAGGCCGTGGGCAAGGAGGAACTAGGAAAGAACATCAATGCGGACGAAGCT GCTGCCATGGGGGCTGTGTACCAGGCAGCGGCGCTCAGCAAGGCCTTCAAAGTGAAGCCATTTGTTGTGC GGGATGCTGTCATTTACCCAATCCTGGTGGAGTTCACAAGGGAGGTGGAGGAGGAGCCTGGGCTCCGAAG CCTGAAACACAATAAGCGTGTGCTCTTCTCCCGAATGGGGCCCTACCCTCAGCGCAAAGTCATCACCTTT AACCGCTACAGCCATGATTTCAACTTCCACATCAACTACGGTGACCTGGGCTTCCTGGGGCCTGAGGATC TTCGGGTATTTGGCTCCCAGAATCTGACCACAGTAAAACTAAAAGGCGTGGGAGAGAGCTTCAAGAAATA TCCCGACTATGAGTCCAAAGGCATCAGGGCCCACTTTAACCTGGATGAGAGTGGCGTGCTCAGTTTAGAC AGGGTGGAGTCCGTATTTGAGACCCTGGTGGAGGATAGCCCAGAGGAAGAATCTACTCTTACCAAACTTG GCAACACCATATCCAGCCTGTTTGGAGGTGGTACCTCATCAGATGCCAAAGAGAATGGTACTGATGCTGT ACAGGAGGAAGAGGAGAGCCCCGCTGAGGGGAGCAAGGATAAGCCTGCAGAGCAGGGGGAACTCAAGGAA GAAGCTGAACCCCCAGCAGAGGAGACCTCTCAGCCTCCACCCTCTGAGCCTAAGGGGGATGCAGCCCGTG AGGGAGAGAAACCTGATGAAAAAGAGAGTGGGGACAAGCCTGAGGCCCAGAAGCCCAATGAGAAGGGGCA AGCAGGGCCTGAGGGTGCTGCTCCAGCTCCTGAGGAGGACAAAAAGCCGAAACCTGCCCGGAAGCAGAAA ATGGTGGAGGAGATAGGTGTGGAGCTGGCTGTCTTGGACCTGCCTGACTTGCCAGAGGATGAGCTGGCCC GTTCTGTGCAGAAACTTGAAGAACTGACCCTGCGCGACCTAGAGAAGCGGGAGAGGGAGAAAGCTGCCAA CAGCTTGAAGGCTTTCATCTTTGAGACCCAGGACAAGCTGTACCAGCCTGAGTACCAGGAAGTGTCCACT GAGGAACAGCGGGAGGAGATCCCGGGGAAACTCAGCGCCACTTCTACCTGGCTGGAGGATGAGGGATTTG GAGCCACCACTGTGATGCTGAAGGACAAGCTGGCTGAGCTGAGAAAGCTGTGCCAAGGGCTGTTTTTTCG GGTGGAAGAACGCAGGAAATGGCCAGAGCGGCTTTCAGCTCTGGATAATCTCCTCAACCATTCCAGCATT TTCCTCAAGGGTGCCCGGCTCATCCCGGAGATGGACCAGGTCTTCACTGAAGTGGAGATGACGACATTAG AGAAAGTCATCAATGACACCTGGGCCTGGAAGAATGCAACTCTGGCCGAACAAGCCAAGCTTCCTGCCAC AGAGAAGCCTGTGCTGCTTTCAAAAGACATTGAGGCCAAAATGATGGCCCTGGACCGGGAGGTACAGTAT CTACTCAATAAGGCCAAGTTTACCAAGCCACGGCCACGGCCCAAAGACAAGAATGGCACCCGGGCAGAAC CTCCCCTCAATGCCAGTGCTGGTGACCAAGAGGAGAAGGTCATTCCACCTGCAGGCCAGACTGAAGAGGC GAAACCCATTTTAGAACCTGACAAAGAAGAGACTGGTACGGAACCAGCAGACTCGGAGCCTCTGGAATTA GGAGGTCCTGGAGCTGGACCTGAACAGGAAGAGCAGTCAGCAGGACAGAAGCGGCCTTCAAAGAACGATG AACTATAACCCCAGCTCCTGCCTTCCGACTTGGCTCCAGCCCCTTCTCCTACCACCTCTATTTATTATAC ATCAGGGTTGGAGTGGGGGTTGGTCCCTCAGGGGCTCAAGTTCCTTCTCTCAGCTGGGACAGGAGGTGGC TGCTCAGTCCTAGAGTAGCTCTATGGGTGTAACCGGCTCTAGTTCTGTCCCCAGCCCCACCCTCTGGTCT CCTACTCTCTGGCCCTGTGTTTGGGAAAAATCACTATTATGTCTTAATTCTTTGCCTGTGGGTAAGTGAG AGAATGGCTGCCAGTAGTTGTTGGAGACCTGGTAGTGGGAAGGATGTTATGGGTCACTGGAAAGTGGCCA GTCTTCACAGCATCCTTTCATCTTTTCCTCTTCCACAAAATCAGTGTCCTGTTTGGACCAGTGTCTACAG AGCCTCAGTCCCACTTGGTTTCTGAGTGCCTCTTTTCTCTTCTGTGTCTTCTCTTCCCTTGTCCTTCCTC CTTGGCTCTTCTGTCCTTTCCTTCCCTGTGCAGACAGGACTATGGTCTTCTTGCCCAAAGACTTTCATCT GGACTGTCTATAGAGTAAATATGCTCTCATGGTTCATGCGACCCCTGGTGGCTGGAGTAAGCGGGGAGCA GGCAAGAAGGGAAAGCCCTGTAGCCCTGAATGGGTTGTTGGACCCAGGACAGTACAGGTCCGGAGAGTGG CTGGCAAGGATGCAAGTATACTGCAGGCACTCGACATCCTTGTCAGCCCCAGTTCCTGTGACACCTACCC CTTCATGCTCTTTCCTCCTTCCCCCTGGGCTGACCAAAAATGTGCTATCTACTGTGAGCCCCTTTCCCAA GACCCTGGGGGACGGAGAGACCATGGAGTGAATGTAAAAATGCCACCTCACTCTCAGGCAAGCTTTGTGG GTAAAGACGAGGGGTCAGGGTTCAACTTGACAGTGTACTTCGCTAGGAAGGAGAGGCCCTTGCCTGCTAT AACCATGCCTAGAGTGCCTGTCCTCCAGTATTGCACCTGTAGCCGGAGCTAGGGCCACCCCAGTGCAGGG GCAAAGCAAACCTGATGTCAGTCGTTTTGTCTTTTCTACCCCAACCTCACCCTCTCACCCTACTTTCCAC CCCCCACCCCGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGCCACATTGGCAGAGGGGCCTATAGGT CCTTCACTGTTCCCTCCTGTGTGTTGGAGTTCTTTAGTTAGCAGTTCAGGCTGGTTGGACAGGTTTGAAT CAAATTGTACTTTGTTCCATTGTTAATTGAGAAACTGTTTCAATAAAATATTCTTTTCTATAGGTTGTAA AAAAAATAAAATAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30012694 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30012694 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19531 same embryo
	EMAGE:19528 same embryo
	EMAGE:19527 same embryo
	EMAGE:19530 same embryo
	EurExpress:euxassay_004962 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source