EMAGE mouse gene expression spatial database

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EMAGE:19531

Nlrx1 NLR family member X1 ( MGI:2429611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004948. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004948_01	euxassay_004948_02	euxassay_004948_03	euxassay_004948_04

euxassay_004948_05	euxassay_004948_06	euxassay_004948_07	euxassay_004948_08	euxassay_004948_09

euxassay_004948_10	euxassay_004948_11	euxassay_004948_12	euxassay_004948_13	euxassay_004948_14

euxassay_004948_15	euxassay_004948_16	euxassay_004948_17	euxassay_004948_18	euxassay_004948_19

euxassay_004948_20	euxassay_004948_21	euxassay_004948_22	euxassay_004948_23	euxassay_004948_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19531_wholemount_strong.wlz

19531_wholemount_moderate.wlz

19531_wholemount_weak.wlz

19531_wholemount_possible.wlz

19531_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19531_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7985
Entity Detected:	Nlrx1, NLR family member X1 ( MGI:2429611)
Sequence:	sense strand is shown >T7985 CTTCCAACAGGGACGACCTGCGCCCGAGTTATTTTCTCTTCCACGCTTCCGTGTCTTCATCTGTAAAATG GGCTTCATCAAGTCCAGACTTGGAGGGCAACAGGAAAGAAAGTGGCTCTAAGTGTTCCAGGCTCCAGTTT TCCTAGGATGAGGTGGGGCTGCCATTTGCCCAGGACCTCTTGGGGCTCTGGCCTGGGAAGAACACCCCAG CTACCAGATGAGCATATCTCCTTCTTGATCCAGTGGAGCTGGCCCTTTAAAGGGGTGCATCCCCTGAGGC CCCCTAGGGCCTTTATCCGTTACCATGGAAACTCGGCAGACAGTGCTCCCCCACCAGGGAGGCATGGGCA GCTGTTCAGGAGCATCTCTGCCACAGAAGCTATCCAAAGGCATCGCCGGAACCTCACCGAGTGGTTTAGC CGACTGCCCAGAGAGGAGCGCCAGTTTGGACCAACCTTTGCTCTAGACACAGTTCATGTTGACCCCGTGA TCCGAGAGAGCACCCCAGATGAGCTGCTTCGCCCGTCCACGGAGCTGGCCACGGGGCATCAGCAAACCCA GGCAGGGCTCCCCCCACTGGCCCTGTCTCAGCTTTTTGACCCGGATTCTTGTGGGCGCCGCGTGCAGACC GTGGTGTTGTATGGGACCGTGGGTACTGGCAAGAGCACGTTGGTACGGAAGATGGTCTTAGACTGGTGTT ACGGGAGACTGCCTGCCTTTGAGCTTCTCATCCCCTTCTCCTGTGAGGACTTGTCATCCCTGGGCTCCAC CCCAGCTTCCCTGTGCCAACTTGTGACCCAGCGTTACACACCCCTGAAAGAGGTGTTGCCCCTGATGACT GCTGCGGGATCCCGCCTGCTCTTTGTGCTCCATGGCTTGGAGCGCCTCAACCTTGACTTCCGGCTGGCAG GCACAGGGCTTTGCAGTGACCCGGAGGAACCCGGGCCACCAGCTGCCATCATAGTCAACCTGCTGCGCAA ATACATGCTTCCCGAGGCCAGCATTCTGGTAACCACCCGGCCTTCCACCATTAGCCGAATCCCTAGCAAG TATGTGGGCCGCTATGGTGAGATCTGTGGCTTCTCTGATACCAACCTGCAGAAGCTCTACTTCCAGCTCC GCCTTAACCAGCCTGACTGTGGGTACGGTGCTGGGGGTGCCAGTGTCTCAGTCACACCAGCTCAGCGCGA CAACCTGATTCAAATGCTCTCCCGGAACCTGGAGGGGCACCACCAGATTGCCGCAGCCTGCTTTCTGCCT TCCTATTGCTGGCTTGTCTGTGCTACTTTGCACTTCCTGCATGCTCCCACACCTGCTGGTCAGACCCTCA CAAGCATCTATACCAGCTTTCTACGCCTGAACTTCAGTGGGGAAACACTGGACAGCACCCACACGTCCAA TCTATCCCTGATGTCCTATGCAGCCCGGACTATGGGCAAGCTGGCCTACGAGGGCGTGCCATCCCGAAAG ACCTACTTCTCTGAAGAGGATGTCCGTGGCTGCCTGGAAGCTGGCATCAAGACAGAGGAAGAGTTTCAAC TGCTTCAGATCTTCCGCAGGGACGCCCTGAGGTTTTTCCTGGCCCCGTGTGTGGAACCAGGGCACCTGGG TACCTTCGTGTTCACCGTGCCCGCCATGCAGGAGTATCTGGCTGCCCTCTACAGCGTGCTTGGTTTGCGC AAGACAGCCCTGCAGCGGGTGGGCAAAGAAGTGGTTGAATTTGTGGGCCGTGTTGGGGAAGATGTCAGCC TGGTATTGGGCATTGTGGCCAAGCTGTTGCCCCTGCGGATTCTGCCTCTGCTCTTCAACTTGCTCAAGGT AGTTCCGCGAGTGTTTGGGCGCATGGTGAGTAAGAGCCGGGAGGCAGTGGCCCAGGCCATGGTGCTGGAG ATGTTCCGGGAGGAAGACTACTACAATGACGATGTTCTGGATCAGATGGGTGCCAGCATCCTGGGTGTGG AGGGCCCCCGGCGCCACCCAGATGAACCCTCTGAGGATGAAGTCTTTGAGCTCTTCCCCATGTTCATGGG CGGACTTCTCTCTGCCCACAACCGGGCGGTGCTGGCTCAGCTTGGCTGTCCCATCAAGAACCTGGATGCC CTGGAGAATGCCCAGGCCATCAAGAAGAAGCTGGGGAAGCTGGGTCGGCAGGTGCTGCCCCCCTCGGAGC TTCTTGACCATCTCTTCTTCCACTATGAGTTCCAGAACCAGCGCTTCTCAGCTGAGGTGCTGGGCTCCCT ACGCCAGCTCAATTTAGCAGGGGTGCGCATGACACCCCTCAAGTGCACAGTGGTAGCCTCTGTACTGGGA AGTGGAAGGCACCCCCTGGATGAGGTGAACTTGGCCTCCTGCCAGCTGGATCCCGCTGGGCTACACACTC TCATGCCTGTCCTCCTGCGTGCCCGGAAACTGGGGTTGCAACTCAACAATCTGGGCCCCGAGGCCTGCAG AGACCTCCGAGACCTGCTCTTACACGATCAATGCCAGATCACCACTCTTAGGCTCTCCAACAACCCACTG ACAGCAGCTGGTGTGGGCTTACTGATGGACGGGCTGGCAGGAAACACTTCGGTGACACACCTGTCTCTGC TGCACACTGACCTTGGAGACGAGGGACTGGAACTGCTGGCTGCCCAGCTGGACCGAAACAAACAACTGCA GGAGCTGAACGTGGCCTACAACGGTGCTGGTGACACAGTGGCTCTGGCCTTGGCTAAGGCTGCTCGGGAG CACCCTTCCCTGGAGCTGCTGCACCTCTACTTCAATGAGCTGAGTTCAGAGGGCCGCCAGGTCCTGCGGG ATTTGGGGGGCTCTGGTGAAGGTGGTGCCCGGGTCGTAGCCTCGCTGACAGAAGGGACGGCGGTGTCTGA GTACTGGTCAGTGATCCTTAGTGAAGTCCAGCGCAACGTCCACAGCTGGGACCCGCTCCGGGTCCAGAGG CATCTCAAGCTGCTGCTCCGTGATCTGGAGGACAGCCGGGGCGCCACCCTTAATCCCTGGCGCAAGGCTC AGCTTCTGCGAGTGGAGGGCGAGGTCAAGACTCTTCTGGAGCAGCTGGGAGGTTCTGGACACTGAGACAG TGACTGCTGTGACAGAGCTGTGGGACCACTGGCTCCCTCTGTGGCTTCCTGGCTTGTACAACTCCTTCCA GAAAGATCCCTTTAATTCTGCGGTCAAAGCAATGGGTGAAACTGTGCTGCCCCCACCCCCAACAAGTCCA GCCTAGAGTTCCCAAGGTCAAATGCGGTGAATTGATGTTGGACTTGGATATGGAACATGTCACTTCTCCC TTAGCCTAGAAGGACTAAGGGTATGGCACGTTAGTGGCCACATTGCCCTGTGATGCCACCGCCAGTCTTG CCTCTTCCTCCTCTGATTGTGTTTGCTATGCTGGGGGGTTCTGCCAGCCAGCAGATGTACTGGACAGCTC TCCATCCTGCCTTACGCTCACCTCTGGGCACAGAGTGCCCTCACACTGGTGCTTTCCCAGTCTCAAATGC ACAGGATCGATGGCTCCAAGGCTGCTAGGGACAAGTGGGCCCACCTTGCTGCTATTAAAAAGCCGTGTGT CTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30012053 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30012053 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19528 same embryo
	EMAGE:19529 same embryo
	EMAGE:19527 same embryo
	EMAGE:19530 same embryo
	EurExpress:euxassay_004948 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source