EMAGE mouse gene expression spatial database

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EMAGE:19578

Hsd3b6 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6 ( MGI:109598)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005060. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005060_01	euxassay_005060_02	euxassay_005060_03	euxassay_005060_04

euxassay_005060_05	euxassay_005060_06	euxassay_005060_07	euxassay_005060_08	euxassay_005060_09

euxassay_005060_10	euxassay_005060_11	euxassay_005060_12	euxassay_005060_13	euxassay_005060_14

euxassay_005060_15	euxassay_005060_16	euxassay_005060_17	euxassay_005060_18	euxassay_005060_19

euxassay_005060_20	euxassay_005060_21	euxassay_005060_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19578_wholemount_strong.wlz

19578_wholemount_moderate.wlz

19578_wholemount_weak.wlz

19578_wholemount_possible.wlz

19578_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19578_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

strong

homogeneous

strong expression: see section 05 06 07 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8009
Entity Detected:	Hsd3b6, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6 ( MGI:109598)
Sequence:	sense strand is shown >T8009 TTTGCTTCCCCCTCTCATCTGAGTTGACTGGCAGTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGC TGCTGCTGCTGCTACTACTGCTTCTGCTGCTGCTGCTGCTGGTTCCTTTGCAGAGATCAGAACCAGCCAT TCCAAGCTTGTATCACCGTGGACTAGGGACCAGCTGGGATACAGAAACCATCCTTCCACAGTTCTAGCTC CAGACTGGGACTGCTGACACCCCACACCTTGTGGCTGACCATCTCCAGGGTCACTGTACATTAATCTCAC ACCAACTGTGATCTGCTTCCTGTGTTGACCATGCCTGGGTGGAGCTGCCTGGTAACTGGAGCAGGAGGGT TTTTGGGCCAGAGGATTGTCCAGTTGTTGATGCAGGAGAAAGATCTGGAGGAGATCAGGGTCCTGGACAA GTTCTTCAGACCAGAAACCAGGGAGCAATTCTTCAACCTAGATACAAACATCAAGGTGACAGTGTTGGAA GGAGACATTCTTGACACCCAGTACCTGAGGAAAGCCTGCCAGGGCATCTCTGTTGTTATCCACACTGCAG CTGTCATTGATGTCACAGGTGTCATTCCCAGGCAGACCATCCTAGATGTCAATCTGAAAGGTACCCAGAA CTTATTGGAGGCCTGTATCCAAGCCAGTGTTCCAGCCTTCATCTTCTCCAGCTCAGTTGATGTTGCAGGG CCCAACTCATACAAGGAGATAATCCTGAATGGCAACGAGGAAGAGCATCATGAAAGCATATGGTCTGATC CATACCCATACAGCAAAAAGATGGCTGAGAAGGCAGTGCTGGCAGCCAATGGGAGCATGCTGAAAATTGG TGGCACTTTGCATACTTGTGCATTAAGGCCCATGTATATTTATGGGGAGAGAAGTCCATTCATTTCTAAC ACAATAATTACGGCCCTCAAAAATAAGAATATTCTCGGTTGTACGGGCAAATTCTCCACAGCCAACCCAG TATATGTGGGTAATGTAGCCTGGGCACACATTCTGGCTGCCAGGGGCCTTCGAGACCCCAAAAAGTCACC AAATATCCAAGGAGAGTTCTACTACATCTCAGATGACACCCCTCACCAAAGCTATGATGATTTAAATTAC ACCCTGAGCAAGGAGTGGGGCTTCTGCCCTGATTCCAGCTGGAGCCTTCCTGTGCCCCTACTGTACTGGC TTGCATTCATGCTGGAAACTGTGAGCTTCCTGCTGAGTCCAATCTACAGATTTATACCTCCCTTTAACCG CCACTTGGTCACACTGACAGGTAGCACGTTCACTTTCTCCTACAAGAAAGCTCAGCGAGATCTGGGCTAT GAGCCACTTGTCAGCTGGGAGGAAGCCAAGCAGAAAACCTCAGAGTGGATCGGGACACTAGTGGAGCAGC ACTGGGAGACACTGGACACAAAGTCTCAGTGATGGGAAGAGGGTGGAGACATGGCTCTGGGTGTTACCAG GTCCTCCAGTAAGGACAAAAGCACAACACAGGTGCTGCTGCCTTCTTTTGACACAGAGGCCAATTTAGTG CCTTAATCAAGTCACCAAAGCCTTGCCAGTCACTGACCCAGCCACAAGCCTTCTTTCTGAAATACCTCCC CAAAGACACACAGGCATCTGTGCCCCAGCTCCTGGGTCCAAGCCTCTCAGCACCTCTTGTACCTCAGAGC TCTCTCCATTATTTTCTCTCCCCATTCAGAGCATGTATAGCCTTCAGAAAATTCTACTTGCTTTATGAAA CCCAATGGAAGAAACAATAAATACTTGTCAATACCTCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30018625 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30018625 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19576 same embryo
	EMAGE:19577 same embryo
	EMAGE:19575 same embryo
	EMAGE:19574 same embryo
	EMAGE:19573 same embryo
	EurExpress:euxassay_005060 same experiment
	MGI:4825471 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source