EMAGE mouse gene expression spatial database

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EMAGE:19581

Acsm3 acyl-CoA synthetase medium-chain family member 3 ( MGI:99538)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006277. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006277_01	euxassay_006277_02	euxassay_006277_03	euxassay_006277_04

euxassay_006277_05	euxassay_006277_06	euxassay_006277_07	euxassay_006277_08	euxassay_006277_09

euxassay_006277_10	euxassay_006277_11	euxassay_006277_12	euxassay_006277_13	euxassay_006277_14

euxassay_006277_15	euxassay_006277_16	euxassay_006277_17	euxassay_006277_18	euxassay_006277_19

euxassay_006277_20	euxassay_006277_21	euxassay_006277_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19581_wholemount_strong.wlz

19581_wholemount_moderate.wlz

19581_wholemount_weak.wlz

19581_wholemount_possible.wlz

19581_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19581_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

physiological umbilical hernia

moderate

regional

moderate expression: see section 10

stomach

moderate

regional

moderate expression: see section 06 07 08

hindgut

moderate

regional

moderate expression: see section 08 09

rectum

moderate

regional

moderate expression: see section 10 11

midgut

moderate

regional

moderate expression: see section 09 12 weak expression: see section 13 14 15 16

bladder

weak

regional

weak expression: see section 11

bladder

moderate

regional

Expression in the fundus region.

urethra of male

weak

regional

weak expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7521
Entity Detected:	Acsm3, acyl-CoA synthetase medium-chain family member 3 ( MGI:99538)
Sequence:	sense strand is shown >T7521 CCACCAACACTAAAGCAGAGGCAATGGACTTGGCCAACATCCTAAAGACCAGTGAATCTCTCACTTGAAC ACAGAAACTTGTGTGGAGAATATTGTTAAAGATTTAATCCATTAACTCAACTGATCTTCAGTGGGACCTA AAGATTATGCACTTCTGACAGGTGTCCAAGTGGTGCAGTGGTTCATAGACCATAGGCTCTGGATGAGTGG ATCTCCACTTGGGTGAAAGACAAGGAGGTTCAAGCTGGATGATCTCAGAGAGTTCAAGATCACCGACTCG TGAGGTTCTCACACAGGAAGTGGACACCTGGAGCCGCTGGATTTCAAATGCATCCATTTCATCAGCATGA TGCCTAGCCACCAGATCCATCTGGATTAGTCTGCATGGAAATCTCACATGCCAAAACATTCAACAACACT GATGCTAGCTAGCTCTTGTCATCATGGTGATGTTACTCCGTGCTAGGTGTTTTCAGCGCCTAGCAATTCC TGATCCTATGAGAGTCTTGTATAAAGATTACAGAACAGCGACCCCTCAGAACTTTTCCAACTATGAGTCC ATGAAACAAGACTTCAAAATAGAGATTCCAGAGTATTTCAATTTTGCAAAAGATGTCCTGGACCAATGGA CCAATATGGAAAAGGCTGGAAAGAGACTTTCCAATCCAGCCTTCTGGTGGATAGATGGGAATGGAGAAGA GCTGAGATGGAGTTTTGAAGAACTTGGGTTGTTATCCAGGAAATTTGCCAACATACTCACAGAAGCCTGC TCCCTGCAAAGAGGAGACAGAGTAATGGTGATACTGCCCAAGATCCCAGAGTGGTGGCTTGCAAATGTGG CCTGTCTGCGAACAGGGACAGTTTTAATTCCAGGAACCACTCAGCTGACCCAGAAAGACATCCTCTATAG ACTACAATCTTCAAAAGCTAAGTGCATTATTACCGATGATACTTTGGCCCCAGCAGTAGATGCCGTGGCA GCTAAATGTGAAAATCTCCACTCCAAATTAATTGTGTCTCAGCACTCCAGAGAAGGCTGGGGGAACCTCA AGGAGATGATGAAATATGCCAGTGACAGCCACACTTGTGTGGACACAAAACACGACGAGATGATGGCCAT CTACTTCACCAGTGGGACAACTGGGCCTCCTAAGATGATTGGACACACCCACAGCAGCTTTGGTTTAGGA TTGTCTGTCAATGGAAGGTTCTGGCTGGATTTGATAGCCTCCGATGTGATGTGGAATACTTCAGATACAG GCTGGGCAAAGTCTGCATGGAGTAGTGTTTTTTCTCCATGGACCCAAGGAGCATGTGTTTTTGCACACTA TTTGCCCCGTTTTGAATCAACTTCCATCTTGCAAACCCTCTCCAAGTTCCCCATCACTGTCTTCTGTTCT GCACCAACTGCCTACCGGATGCTTGTTCAGAATGACATGAGCAGCTATAAGTTCAACAGTTTGAAGCACT GTGTCAGTGCTGGAGAACCTATTAACCCTGAAGTGATGGAACAATGGAGAAAGAAGACGGGCCTAGACAT CTATGAAGGATATGGACAGACAGAAACGGTGCTGATCTGTGGAAATTTCAAGGGGATGAAAATTAAGCCC GGCTCAATGGGAAAGCCTTCTCCTGCTTTTGATGTGAAGATTTTAGATGAAAATGGTGCCACTCTTCCTC CTGGACAAGAAGGGGATATTGCTCTTCAAGTTCTTCCTGAGCGACCATTTGGCCTTTTTACTCATTATGT AGATAATCCTTCCAAAACGGCTTCAACTCTACGAGGGAGTTTCTACATTACTGGGGACAGAGGATATATG GATGAAGATGGCTATTTCTGGTTTGTTGCAAGATCAGATGATATCATATTATCTTCTGGTTACCGAATTG GACCATTTGAGGTAGAAAGTGCCCTCATAGAACATCCTTCCATCGCAGAGTCAGCTGTTGTCAGCAGTCC AGACCCCATCAGAGGAGAGGTAGTAAAGGCTTTTATTGTCCTGAATCCTGATTACAAGTCCCATGATCAA GAGCAACTGAAAAAGGAGATCCAGGAGCACGTGAAGAAGACTACAGCGCCTTACAAATACCCCAGAAAGG TAGAATTTATTGAAGAGCTGCCGAAGACTGTCAGTGGGAAGGTCAAAAGAAATGAACTGAGGAAAAAGGA ATGGGTAACAACTTGAACTCCTCCCACTAATCACCTGTGTAGCTATAAACACAGTGTCTACAAGTCTGCA GGAACCACAGATTATGTGGGCAGACTGCTTTGAAACTGGATTGAAAGTATTTTGTAGTTATGACATCAGA GCCTGAATTCTGAAATCAAATAATCATTATTGAATCCTTCTGCATTCATGTCAATGCTCCTGTAATCTGC CAACATAAAATGAATGTCGCCAGATGCTGAGATGTCTTAAAATTTATTTTAGGAGCAGATTCTAAACTAA ATCTGTACCCAGGCATTTTCAAAACATAACTGAACAATTCTAATATGCTAATATACAAATCAGAACAAAT CTTCAAACTTTGAAAAGAACCCTATTTTTAAGAAACTGTCATAATTCTTAGAATATTTACCTAAAACTTT ACTAGTCATATTTTTATTCAAGTAATTTAAAAATAGTTTGAAATTTGTTTGAAGGTCAAAGTATAAGAGA AAGCTGTCACATTCTGTGGCTAGTTATTAAATTTAACCTAATAAACATGGTTTGTAAAAATAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4239288 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239288 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19580 same embryo
	EMAGE:19579 same embryo
	EMAGE:19582 same embryo
	EMAGE:19583 same embryo
	EurExpress:euxassay_006277 same experiment
	MGI:4822950 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source