EMAGE mouse gene expression spatial database

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EMAGE:19599

Nufip1 nuclear fragile X mental retardation protein interacting protein 1 ( MGI:1351474)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004963. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004963_01	euxassay_004963_02	euxassay_004963_03	euxassay_004963_04

euxassay_004963_05	euxassay_004963_06	euxassay_004963_07	euxassay_004963_08	euxassay_004963_09

euxassay_004963_10	euxassay_004963_11	euxassay_004963_12	euxassay_004963_13	euxassay_004963_14

euxassay_004963_15	euxassay_004963_16	euxassay_004963_17	euxassay_004963_18	euxassay_004963_19

euxassay_004963_20	euxassay_004963_21	euxassay_004963_22	euxassay_004963_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19599_wholemount_strong.wlz

19599_wholemount_moderate.wlz

19599_wholemount_weak.wlz

19599_wholemount_possible.wlz

19599_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19599_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8013
Entity Detected:	Nufip1, nuclear fragile X mental retardation protein interacting protein 1 ( MGI:1351474)
Sequence:	sense strand is shown >T8013 GACAAGAGGTAATCCCTTGCTAGGTGGCATGGCTGAACCGGCACCCGCCGTGTGGCCTTCGGCCCCGGAT CTGACTCCCGCGCCCGGGACTCCGAGCGAAGCTGCGCCGCCGCGGGACAACTGGGTGTACTGGGCCATGC TGCCGCCGCCACCACCTCCTCTGTCGTCCCCAGTGGCGGGCTCGGAGCAGAGCCGGAAGGGACAGCCTCA CGTGCTGCCCCAGCCTCCCTCCGGAGCGCTTCCACCGTTCGATGCTCAGATTCTTCCCGCGGCGCAGCCT CCTTTCGATGCCCAGGCTCCGCCCGATGCTCAATCTCAGTTCAGCGGCCAGCAGGCCTGGAATTTGCAAG CCTCCACGCCTTGGTACTGGGGATTGTCTCCTAATGGTTTTTCCACGTACCACACGTCTTACCAATCTCC GGTTACACATTCTTATTTTCCACGATCACATGATGCAAAATTCAATTTGCCTCAAAACAGAAAACAGAAA ACGAAGAAAAGAAAGGAACCGGTTTTCCATTTTTTCTGTGATACCTGTGATCGTGGCTTTAAAAATCAAG AAAAGTATGACACACACATGTCTGAACATACAAAATGCCCTGAAGTAGATTGCTCCTTTAGTGCACATGA GAAGATTGTCCAGTTCCATTGGAGAAATATGCATGCTCCTGGTATGAAGAAGATCAAGCTAGACACTCCA GAGGAGATTGCAAGATGGAGGGAAGAGAGACGAAAAAACTACCCAACTCTGGCCAATATTGAAAGGAAGA AAAAGTTACAACTGGAAAAGGCAAAGAGAGGAGAAGTGTTGACAACCACACAGTATGGCAAAATGAAGGG CATGTCCAGACATTCACAGATGGCAAAGATCAGAAGTCCTGGTAAACATCATAAATGGAGAAGAGGTGGG GCTAGGCAGAGAGCAGTCGTTGGACTAGGAAATCATGCCAGAGATTCGAAGCCTGAAGTTCCATCCAAGG CTAATGTGGACCCTCTAGGTGCTTTGATCCACAGCGACTCTGAGTCTGATAAGGAAGAGAAAGCGCAAAG GACTGTAGTGCCCAAGGAAGTGACACCAGCCTTGTGTTCGCTGATGAGCAGCTACGGCGATGTCTCGGGG TCAGACAGTGAGCCAGAAGAAGCTCCCATCAAGACTGAAGCAGAAGTTCTGGCAGAAAACCATGTTCTTC ATAGCAGTCCTCCCAAGAGTCCAAAACAAAATGTTCAAACAACTGGTAGAACAGTCTCAAGATCCAAGTG GGAGAACCAGAGGAACGGACTCAGAAAGATAAGCCTTAAGCGGAAAAAGAGCCACTGTCACCCATTATTT GAACCAAGAACACATCACCCATATCTCCTGGAAATGCTTCTAGCTCCAGATATTCGACATGAAAGAAATG TGATTTTGCAGTGTGTTCGATATATCATCAAAAAAGACTTTTTTGGACTTAATACTGATTCTGTGAAAAC TGAAGAAGTGTAGATGTCTCCCGTTTCCACACTCATCTGTAAAGCACCTGAGAGAACACCATCCTTGAGT AAGGAGAGAAAATCAGCAACTTCTGCAGCTGGGATCAGTAACTGAACTGGAAGCCTCATGGGCGTCATGT TGGATTTTAAGTCTGTCCTCAGGCTTTATGCAAATAAATGCATAGCTGGTCTTTTTTAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30024494 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30024494 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19602 same embryo
	EMAGE:19601 same embryo
	EMAGE:19598 same embryo
	EMAGE:19600 same embryo
	EurExpress:euxassay_004963 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source