EMAGE mouse gene expression spatial database

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EMAGE:19600

P2ry10 purinergic receptor P2Y, G-protein coupled 10 ( MGI:1926076)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005021. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005021_01	euxassay_005021_02	euxassay_005021_03	euxassay_005021_04

euxassay_005021_05	euxassay_005021_06	euxassay_005021_07	euxassay_005021_08	euxassay_005021_09

euxassay_005021_10	euxassay_005021_11	euxassay_005021_12	euxassay_005021_13	euxassay_005021_14

euxassay_005021_15	euxassay_005021_16	euxassay_005021_17	euxassay_005021_18	euxassay_005021_19

euxassay_005021_20	euxassay_005021_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19600_wholemount_strong.wlz

19600_wholemount_moderate.wlz

19600_wholemount_weak.wlz

19600_wholemount_possible.wlz

19600_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19600_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 09 10 12 moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8062
Entity Detected:	P2ry10, purinergic receptor P2Y, G-protein coupled 10 ( MGI:1926076)
Sequence:	sense strand is shown >T8062 AAGAGGTAATCCAGGATATGTGCTTGCTGCCCAGAGTTGTACCTTTTTACTCTCATCTCTTTGCAAATAT ACAAGACTCTATCCCAGCTCAGCATCAGGGGGCCATAATTCCACAGTTTTCCTTGCCAAACATACTGAAA TATTCAAGATGGGCAGCAACAGTACCAGCAGTGCTGAGAGCAATTGCAATGCCACTTATTTGCCATTTCA GTACTCTCTGTATGCAACTACCTATATCTTCATATTCATCCCGGGTCTCCTGGCTAACAGTGCAGCCCTG TGGGTTCTGTGCCGCTTCATCAGCAAGAAGTACAAGGCCATCATTTTCATGATCAACCTCTCAGTGGCAG ACCTTGCTCATATCCTGTCCTTACCTCTTCGGATTTACTATTATATCAATCGTCACTGGCCATTCCAGAG GGCCCTTTGCCTGCTGTGTTTCTATCTGAAATATCTCAACATGTATGCCAGCATTTTTTTCTTGACATGC ATTAGCCTTCAGAGGTGCCTCTTTCTCCTCAAACCATTCAGAGCCAGAAACTGGAAGCGTAGGTACGATG TGGGCATCAGTGCTGTCATCTGGATTGTTGTAGGGACTGCCTGTTTGCCATTTCCCATCCTGAGAAATGC TGGCTTAGCCAATAGCACAGATTCTCGCTTTGCTGATCTTGGGTACAAACAGATGGATGCAGTGGTTCTG GTCACCATGGTTGTAATTGCTGAACTTGCTGGATTTGTGATTCCAGTGATCACCATTGCATGTTGTACCT GGAAAACAACTGTATCCTTGAAACACCCACCAATTGCTTTTCAAGGGATTAGTGAGAGGAAAAAAGCATT GCGGATGGTTTTCATGTGTGCTGCAGTCTTCGTTATCTGCTTCACTCCTTATCACATTAACTTCATTTTT TACACCATGGTAAAGGAAAGCATCATTACCAGTTGTCCCACTGTAAAAAGTACCCTGTATTTCCATCCTT TTTCCCTATGCCTTGCAAGTCTCTGTTGTCTTTTGGACCCAATTCTGTACTATTTCATGGCTTCAGAGTT TCGTGACCAACTATCTCGTCATGGAAGCTCTGTTACTCGTTCTCGTCTCATGAGCAGGGAGAGTGGATCA TCAATGGTTAACTAAAATGAGAAAATTCTTCAGTTAGGATTCTCTTTACCTGACATTCTGTGGCATTGCC CAAAGGCAGGAATTTCCAGGCAAATAATTCCTTTAACTGAACCTTGGAAATAGCAGAAATAAGTTTTATT GTGTGATGGTTGTGGTCTCCACAGATGATGGAGGCATAATGTGAAAAAATGTGGGAGTAGAAAGAAAAAT GGGATCCATGTGCTTCCAATTTAAAATTTATGGCATTATATTAGTTACAAGTCCCAATTTAAGTTCAAGT TTTTAGCAGATGTTTCCATCCAAATATAAATTGAATGCATATATTTAAATTCGTCTTCAGTAAAGAGAAT AGTACTGAGATGCAAGGTATATATTAAGATGTACAGAAGAATAAATTTCCCCCACGGAATAATTTTATTT CTGAATGGAGTAAAGATTTCAAGTCTTTATAAAACATTACACACAGACAAAATGACACAAAAGTTTCAAT ATGTGAATGAATTTTAGCACTGCACTCTTGCTACTGAATGGTGTACATGAAAATCCACCTCATGGATGTT CGTAGATGTCCATCATTTTCTAGGTTGGCAGTTTGCTATGTATTTTTACAAATAATATTTCATCTTACTT TTCCAATACAAGCAACAGTAAATGAGGAGATTACAAATACAAATGAATGTAAATGTAATGGCAGAGAGGT TAAGCAACATGTCTCACAATTAACAGTTACATGGCATATACTTATGCTATTTCCTCTGCTTATATTTTTA TATGTATTAGGTCAATACTAAGTTTGAGTTGAATGTGGAGGACACAAACAGAAGATAAGCATAGGAAATA GAGATAGTTTTAACACAATTTTCCTGTGCTCATCGAAAAATCTATAATTTGGGGATTAGCATGATTCTGG GTAGAGTCTTCTGTGATAAATATATCGTCATATTAGTGTAATCATCTATATAGTCTTGATGAGAAAAAGC TCAATAATTCATTATTTCAAAATCAATATTATGGCTAATATAAGCCATGCAAATAACATGAATAAAAAGC TATGAAACTGAGCCGCATTTTAGAAAATGTAGGCTTCCTTTTAAAAATTCTCTTTTTAAAAAAATTCTTA CACATTACTATGTCACTAAATAAAATCTCAATGGTTTTCAGATATCATAACTCACATAATTGCATTAGAA ACAGAGTATTAATCACATTGTGAACAAATATGAGAGAAATATGTGGCCTTGTTTTTTTCTGAGTGTTATG GATACTATTTTAAGTGTTCATATAGATGATACAAATAGAACAGAACCCCCATCTTCTCAAGTTGACAATT CACTGGTATTTTAGGTCTGAAATGAACAGAGCATAGACATTTGGTATGCAGGCAAGAAAAGGAAGTGGTA GTACTTAGAACATGCATTTGTGTAGACTAAGGAAGAGGAAGGGACCACCAAAAGGAGGAATTAAAAGAAA ATTTCTTGGTTTTGGGGGAATGAGATAGTTCTGTAATTATAATGTAATAAAGTGTTTTTTAAAATTTGGA AAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30072257 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30072257 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19602 same embryo
	EMAGE:19599 same embryo
	EMAGE:19601 same embryo
	EMAGE:19598 same embryo
	EurExpress:euxassay_005021 same experiment
	MGI:4827015 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source