EMAGE mouse gene expression spatial database

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EMAGE:19650

Nrsn1 neurensin 1 ( MGI:894662)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005168. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005168_01	euxassay_005168_02	euxassay_005168_03	euxassay_005168_04

euxassay_005168_05	euxassay_005168_06	euxassay_005168_07	euxassay_005168_08	euxassay_005168_09

euxassay_005168_10	euxassay_005168_11	euxassay_005168_12	euxassay_005168_13	euxassay_005168_14

euxassay_005168_15	euxassay_005168_16	euxassay_005168_17	euxassay_005168_18	euxassay_005168_19

euxassay_005168_20	euxassay_005168_21	euxassay_005168_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19650_wholemount_strong.wlz

19650_wholemount_moderate.wlz

19650_wholemount_weak.wlz

19650_wholemount_possible.wlz

19650_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19650_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20 21

olfactory lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 08 09 10 13 14 15 16 18 19 20 21 22

midbrain mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

facial vii ganglion

strong

regional

strong expression: see section 03 04 17 18 moderate expression: see section 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 15 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 05 06 15

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 18 weak expression: see section 15

not examined

regional

not examined expression: see section 09

trigeminal v nerve

strong

regional

strong expression: see section 07 08 14 15

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 13

cervical ganglion

strong

regional

strong expression: see section 06 moderate expression: see section 07 14

thoracic ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 12 13 14

tongue

strong

regional

strong expression: see section 10 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8536
Entity Detected:	Nrsn1, neurensin 1 ( MGI:894662)
Sequence:	sense strand is shown >T8536 AGAAAGACCAAAGAATAAACAGGCAGAGGACAAGGAGAACATTGAAGCTGGGAAGATGACTTCTTGCAGC AACACCTGTGGGTCCAGGCGAGCCCAGGCTGACACCGAGGGTGGGTACCAGCAGCGCTATGGAGTTCGGT CCTACCTGCATCAGTTTTATGAGGACTGCACTGCCTCTATCTGGGAGTATGAGGATGATTTCCAGATCCA GAGATCGCCTAACAGGTGGAGCTCAGTATTCTGGAAGGTCGGACTCATCTCAGGCACTGTCTTTGTGATT CTCGGACTAACTGTCCTGGCAGTGGGCTTTCTTGTGCCCCCCAAAATCGAAGCTTTTGGCGAAGCTGATT TCATGGTGGTGGACACACATGCTGTGAAGTACAACGGCGCCCTGGACACATGCAAGCTGGCGGGGGCTGT GCTCTTCTGCATCGGGGGTACATCCATGGCAGGGTGCCTACTGATGTCTGTGTTTGCCAAGAGCTATTCC AAAGAAGAAAAGTTCCTTCAACAGAAGTTTAAAGAGCGAATCGCAGACATTAAAGCCCATACCCAGCCCA TCACTAAAGCTCCAGGCCCAGGGGACACCAAGATCCCTGTCACTTTGTCCAGAGTTCAGAATGTGCAGCC TCTATCAGCCACTTGAAACATTTGTCACCTCAGGCCTCCCCTCTCTGATTCACATGCATCCTCGTTTTAA AAAGGTAGCCAGTCAGTTTTTGAGATAACATGCATTTGGCAGTGACCTATCTATCCCTAGAGCTGTGTGG GGCTGGACAGGGTGTCATGCAGACTTACATGCACTTCAGGTGACAGTGGATGTTATGTGTCCGTTCGTAC CTGCTTCTGGTGGGTTCCACAAGCCCCTCTTGAACTAGTGTCTCTGGTGTATCAATGTTGTATCATTTTC TGTGTTACTTGAAAGCACTGAGCAGAGTAGACCAGCCTACCAGTCGTCAACGTGACCCACTTCATTCTCT AATAGGTTTTTCTGATCCCTCCGTGTGGTGCTGTCTTCCATGCCTATTTCTGTGTATGATACCATCATGT GTTCCTAGAATTGCAGTTCCCTGATTTTGATCCAGATGTTAGACTTTTAACTCTATCTTAGCATAAAAGT CCTAAAAGTGAGCAGGAATTTATCAGTGTCCTTTGTAAGTCATTTTGTTTCCTCCAGATGTTGTTTCTTT TCACTTAAGTAAAATCGCCAGTTTTGGAATCTTGGTAAAGGCAGTATCAACAATGAGAGTGGGAGTATAC ATTTAGGCAGCATCTAAAAACAGTTAAACACTGGAAAACTTGAGTTTCTGCCAGTACCTTTGCCATGTGA AAAAAAAAAATGTTCCCCAGACCTGTTTTCCCTCCTGTGTAAGAGGGTTTCAGAACCTTACCTGCTCAAG TATCCAAATTTATTTTCCTGTGTCACTGAGTCTCTTTCCTGAGGATCTTGTGATGGTTTCCTGTTGGGGT ATGGTCAGAACTGGACTCCTGGCACCTCCAACTCTGCTATTTTTCAGTATTTACCCAAAATACCCAACCA AGCAGATTGGATGATGCCAATGTGTTTCTATAGAGACGAGTATGCTATTCTCAAATAAGAAACCCCAAGG TGGTGTCAACCTTAAAAAGCATTTAAATCGTCAAACAGGAAAAACGGAGCCCCAGATCCCTTTGTTCCTT GCATTTTCATTTAATCTGAAAATGTAGATTAGGCAGAGGAGAAGGGAAAGTCTGACTCTGGTATTCTCTC AACTGTGCTCCTTAGAGTTTTGTCTTTGTGTGTATACATAGTCTTCATCTCAACAGTAAATAACACATGT GAATGGGCCCAACCAGAACTGCACTGTCATCCCTCCAGTGTAGCTCTTTTCTCTCCACCATGTAATTTTC CATTCTGAGACTGAATAAGCTCTGATGTTTCCCTGACTAAATGGAAGAATAAATGAAGCAATTAGTGGTC AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5708003 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5708003 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19651 same embryo
	EMAGE:19653 same embryo
	EMAGE:19649 same embryo
	EMAGE:19652 same embryo
	EMAGE:19648 same embryo
	EurExpress:euxassay_005168 same experiment
	MGI:4826799 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source