EMAGE mouse gene expression spatial database

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EMAGE:19651

Aggf1 angiogenic factor with G patch and FHA domains 1 ( MGI:1913799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005113. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005113_01	euxassay_005113_02	euxassay_005113_03	euxassay_005113_04

euxassay_005113_05	euxassay_005113_06	euxassay_005113_07	euxassay_005113_08	euxassay_005113_09

euxassay_005113_10	euxassay_005113_11	euxassay_005113_12	euxassay_005113_13	euxassay_005113_14

euxassay_005113_15	euxassay_005113_16	euxassay_005113_17	euxassay_005113_18	euxassay_005113_19

euxassay_005113_20	euxassay_005113_21	euxassay_005113_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19651_wholemount_strong.wlz

19651_wholemount_moderate.wlz

19651_wholemount_weak.wlz

19651_wholemount_possible.wlz

19651_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19651_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8590
Entity Detected:	Aggf1, angiogenic factor with G patch and FHA domains 1 ( MGI:1913799)
Sequence:	sense strand is shown >T8590 CTTCCGCTGGGTTCGCTTTAGTTCTCCCGTCCGCTGGCTTCCCGGAGACTGTCACCGGGCGGGTGTCCGC GCGGCCCGGGTAGAGTCGGCTTCGTCCCCAAGCTCGCAGTCCCGGCCGCCGTCTTCGGGGGCGACATGGC CGGGGCCCGCTGCGGTCGTCCGAGCGTGAGGCCGCCCCTCGCAGCCCGCGCGCTCCAGTGGCCCCTGGGC CAGTGGGCCCCGGTGTCGTCCTAAGCAGCCCGGTCCGCGCCCCGCGGGCTCGGCGCTCACGGCCGCATGG CCTCCGAGGCGCCCTCGCCGCCTTCGCCTTCTCCGCCGCCGCCCGCCTCTCCGGAGCCGGAGCTGGCGCA GCTCAGGCGGAAGGTGGAGAAGTTGGAGCGCGAGCTGCGGAGCTGCCGGAGGCAGGTGCGGGAGGTGGAG AAGCTGCTGCAGCACACGGAGCGACTCTACCGCAACGCCGAGAGCGACAACCAGGAGCTCCGCACGCAGG TAGAAGAACTTAGTAAAATACTCCATTGTGGGAAAAATGAAGATAATCCGAAGTCTGATGTAGAAGTACA GACAGAGAGCCAAGCTCCTTGGGCGATTTCAGATTATTACTATCAGACATGTTATAATGACGACAGTCTT CCCAGTAAAGAGACGGAGCTGTGTGTACAGCAGAGTCAGTGTGCTCAGGCTTCCGCTCTTGATCCTCAGG ACGAGTCACACATAGACAGCGGGAGTTATGCTGGTGCTGATGCCACAGAAGGTGTTAGCCATAGACAGGA GGACGCCGTCACCTCTGACTCACAGGAGAGTGTGTCCGCGCTAGCAGAAGGCCCAGCACTCGAAGGGTCC TCGCTTGCTGAGAGCTTGAGAGCTGCAGCGGAGGCTGCTGTGTCGCAGACCGGCTTCACCTACGACGAGA GCACGGGCTTATATTTTGACCACAGCACTGGTTTCTATTATGATTCTGAAAACCAGCTGTATTATGACCC TTCCACGGGGATTTATTACTACTGCGATGTGGAGAGTGGTCGGTACCAGTTTCACTCTCGCGTAGACCTG CAGCCTTACCAGACCTCTAGCACAAAACCAAACAGAGAAAGAAGACTGAAGAAGAGAAGAAAGGAGCCAG GTTTTTATACAGCAAATGAAGAAAAGGATTTGAGCTCAGAAGATCAGAAAGTCTGCAGTGTAGAATATAT AAACTGCAGTGAGGATGAACATTCTGGAAATGTGAAAAAGAAGGCCAGAACAGACACTTCTCACAAAAGC AGTCCCTTACAGCTCACGGTGGCAGTTAGTGGAGACACTGTGGAGTCTCCTGGAGATGATAACTCAGCGT CATCTAAGGATGAGAGAATCGGAGAGAGTGAGAGCGAGCCGGAAGAAGGTGAGATCACAGACTCTCAGAG TGAGAAGAGCTATGATGGAGACAGTAGCAGTGGGGACAGGGAGACCTCAGAAGAATCCGACGATGAAGAT GAGGAAAGAATTTGGCCGCCCTGTATTCGCGTGATTGTCATTAGGTCTCCAGTGTTGCAGATGGGCTCGC TGTTCATCATCACCGCTGTGAGCCCAGCCACCATTGGGAGAGAGAAGGACATGGAGCATACTGTGAGAAT CCCTGAAGTCGCTGTTAGTAAGTTCCACGCAGAAGTTTACTTCGACCATGACTTGCAAAGCTACGTTCTT GTGGATCAGGGCAGCCAGAATGGTACCATTGTCAACGGGAAACAGATTCTTCAGCCAAAAACTAAATGTG ATCCTTACGTCCTCGAACACGGCGACGAAGTGAAAATTGGGGAGACTGTGCTGTCTTTTCACATTCACCC TGGCAGTGAGACGTGCGATGGCTGTGAGCCGGGGCAGGTCAGAGCTCACCTCCGCCTCGATAGAAAGGAC GAGCCTCTGGTCGGTCCAGCACTAAGTAAGGAGGAAAAAGAGTTGGAAAGAAGAAAAGCACTCAAGAAAA TACGAGTAAAGTATGGCTTGCAGAATACAGATTATGAAGCTGAAAAAGCGTTGAAGAATCCTAAGTATAA AGACAGAGCTGGAAAACGCAGGGAGCAGGTGGGAAGCGAAGGGACTTTCCAAAGAGATGACGCCCCTGCG TCTGTTCACTCTGAAATTACAGATAGCAACAAAGGCCGAAAGATGTTGGAGAAGATGGGGTGGAAACGGG GAGAAGGCCTGGGAAAGGACGGTGGAGGGATGAAAACGCCGATCCAGCTTCAGCTTCGACGGACACATGC TGGCTTGGGGACAGGGAAGCTGTCCTCGATTGATGACGTTCACCTCATCCAGAATAAGAGCAAAAAACAC TGGGACAAAGCCCGGGAGCGGTTTGCGGAAACTTTCACAGAAAACAAACCTCGGAAAGAGACCCCAGGGG CAGTGCCGTGGGTGACAGGGACTGCAGAGTAAAGGCCTGTCTGTCACACGGGAACTTGGAGCTTCAAAAG AGAAAGAGTTTGAAAACTTCAGTGTAGAAGCTGTGTTCCTAGAAAGTCAGTCACTGGAGGAACTCTTAAG ATGGCTACCTGATCGACACATGTGTGGGGACATGTGGTTTGTAGCTTGTAGAAAGCAGTTCTTAAGTGGG CTCAAGGTGACAGGCACAGGAGAGCTGCCAGACCTTTGTTGTTGTGGCCTGTCACTCACCCTGTGGAGAG CAGCTGACCATGTGAGCAGCGCACATAACCACTAATGACTTCAGCGCGCGTGTCCTGTCTCTGTCATCAT TACCATGACACACAGATATCAGACATTTATAAAACAATTCTATGTGTATACTTGTTTACACCTTTTAGAG CTTAGGTTTTTTTTTTAAAAAGTCGAGAAACCATGGGTAGTGGAGCCTAATTTTACTATCCTTGAAATAA CTGCAGTAATAATAGTGAAGAATTGATGACAGGTGACAGATTGTAGGAAATTAGTCTCACACTATTTTCT TCTGTGAAGAATGTTGTTTGTACTACAGAGTCAGCTTTGCCTTTGGTTTGTCCTAGCTGATGGAGTATTT CATATAGACGACTGAGTGCAGTGCTGGCATATTCAGTATGCTAGCATTTTTAGTTTTGATAAATACCATT GCAGGCAATGGGACTGTGCTCGAGAAATCTGATGACTAGGGCAGATGGGTTACTAGGCCAAGGCTTCAAA CATTTACTGGAAATGTCTTCAAATGCAATAAAAAAAAACATTTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5716440 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5716440 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19653 same embryo
	EMAGE:19649 same embryo
	EMAGE:19652 same embryo
	EMAGE:19648 same embryo
	EMAGE:19650 same embryo
	EurExpress:euxassay_005113 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source