EMAGE mouse gene expression spatial database

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EMAGE:19672

Fam63a family with sequence similarity 63, member A ( MGI:1922257)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006104. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006104_01	euxassay_006104_02	euxassay_006104_03	euxassay_006104_04

euxassay_006104_05	euxassay_006104_06	euxassay_006104_07	euxassay_006104_08	euxassay_006104_09

euxassay_006104_10	euxassay_006104_11	euxassay_006104_12	euxassay_006104_13	euxassay_006104_14

euxassay_006104_15	euxassay_006104_16	euxassay_006104_17	euxassay_006104_18	euxassay_006104_19

euxassay_006104_20	euxassay_006104_21	euxassay_006104_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19672_wholemount_strong.wlz

19672_wholemount_moderate.wlz

19672_wholemount_weak.wlz

19672_wholemount_possible.wlz

19672_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19672_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8549
Entity Detected:	Fam63a, family with sequence similarity 63, member A ( MGI:1922257)
Sequence:	sense strand is shown >T8549 CTGCTGACCGGCTCAGACAGCCCTGATGGAAATCCTCGGTAGCTGAGCAGGCCCCTCCTCCTCCTCCTCC TCTAAGGACCGCGTCCTTGGCTAATTTGTCTTTGTCCGGACAGGGGGAAAAGAGGGACAGAGAGAAGAAA AAGAAAAACCACAAACTTCCTTTGAAAGCCAGTTTGTAGGCACGGCCTGGAGCGCACGCCGGAGGCTCGG TGACTCGGGAATCCTGAAGACTCTGGGAAGCCAGGCGGGACCGCCTGCGCTGTGCCTTGCTTGTCCTCGG CCCCGACCCTGCTCCAGCGTCCCCCGTATTGGGCGTGTGTCCGGAAGTGGCAGCGACCCAGGCTGGACGC CGCGAGAGTAAACCCGTGTCAAGCCGTGTAAGCTCTGGGGCTGACAGCTGGGAGGCGGCTGGCGCAGTCG GCGCCCCTCAACGATGTCCCCACCTCCCGCGTAGAACCGCCAGAGTTGAGTCCTCTCCCAGTGCCGGAAC CTCATCTACCTCTTTGAGGGAAGAAGCCAGCTGCACCCAGTATCTCCATCCTTTCCAAGCCTGATAACCC ATGTCCAGTCGGCCCCATCTGGTCACAGCAAGTCAGAGGTGGAATGAGCTGAATCCTGTTTATACACTCT GAGAAAAACTTAATCTGGAGCCAGAGTAGCCTACACAGTTCTCTTTGACTTTTCTTGTTCCCTTTGCTCT GTAATTCAGTGACCTTGTAGGCCCAGTCTCTCTTCTACACACTAGAATAGCCATAGGCAAGGTCTCTGAG GTCTCAGAACACTTCACTCCCCTCAGCTGACTGTACCCACTATGGAACAACCTCAGACTGAGAACCCAGC CCCTAGTAAGGCCACTAGTGCAGAAACTGTTGAATCTGAAAACCATGAGGCCCTGTCTGGACCAGAGAAG CATCCTCAGGACAAGGATGGTGCAGATGCTGATGGAGCAGCTGGAGAACAGGAGCCAGGAGACCAAACTT TGCCACCAGCCCAGGATGGTGAAAATCTTGAGTGTCCTCCTCCTGAAGCTAGCTCAAGTCCTCCTGGGCC AGCTTGTGGGACGTCACCTAAGGTAGAGACAGCAGAGGTGTGTTCCAGGCCACAGGAACTTCCCCAGTCC CCCAGGATCCAACAGCCTGAGCTAGATTTCTACTGTGTCAAGTGGATCCCCTGGAAGGGAGAACGGACCC CGATCATCACCCAGAGCACCAATGGTCCGTGTCCTCTTCTAGCCATCATGAACATCCTTTTTCTTCAGTG GAAGGTGAAGCTGCCCCCTCAGAAGGAAGTGATCACATCAGATGAGCTCCTGACACACCTTGGAAACTGC CTTCTGTCCATCAAACCCCAAGAGAAGTCTGAGGGCCTTCAACTTAACTTTCAGCAGAATGTGGACGATG CAATGACGGTGCTCCCGAAACTGGCCACAGGTCTGGACGTCAATGTGCGATTCACGGGCGTCTCCGACTT TGAGTATACGCCGGAATGCAGCATCTTTGACTTGCTGGGAATACCTCTATACCATGGCTGGCTGGTGGAT CCACAGAGTCCCGAGGCTGTGAGTGCTGTTGGGAAACTGAGTTACAACCAGCTGGTAGAGAAGATCATCA CCTGTAAACACTCTAGTGACTCCAACCTCGTGACAGAAGGCTTGGTTGCGGAGCAGTTCCTGGAGACCAC TGCGGCACAGTTGACCTACCATGGCCTGTGCGAGCTCACAGCAGCTGCCACGGAAGATGAACTTAGTGTC TTCTTTCGAAACAACCACTTCAGCACGATGGCTAAGCACAAGAGTCACCTGTACCTACTGGTCACTGACC AAGGTTTTCTACAGGAGGAGCAGGTGGTATGGGAGAGCTTGCATAACGTGGATGGTGACAGCTGCTTTTG TGACTCGGACTTCCACCTAAGTCATTCCCTAGGCAAAAGCCATGGAGCAGAAGGTGGGGGTGGCTCTCCA GAGAAGCAGCTGCAAGTGGACCAGGACTACCTTATTGCCTTATCCCTGCAGCAGCAGCAGCAGCCACAAG GCACGTTGGGCCTCAGTGACCTGGAACTGGCCCAACAACTTCAACAAGAAGAGTATCAACAACAGCAAGC AGTTCAACCTGTGCGAACAAGGGCTCCTTCGCCCCAGGGGAGAGGAGCCACATCTGGACGTCCAGCTGGA GAGCGGCGGCAGAGGTCAAAGACTGAGTCAGACTGTGTTCTGCTCTGACTCTGCCCCCTACAGCCAGCTC AGGCTGTGACTTGCAGGATCCCACCCCATCTCAATCCCTGAGACACAGAGAATAACCTATATGCACTGTT GGGGATCAGAACAGACAATAGGTGCCAAGGTCAGACTTGGTACCATCTTTGCCCTTGTACTGCTATCTTC TCTTCCTCTCTTCCAAGGCCACATAGGTGTGGGTGGGTGAAAATCTCTGGTGCCCCACCCCCTTTCCTGG AATAGTCCCGTGGATACATGGACCCAGGCTGCTTGCTGGTCAAGACACCTATCTGGAAAGCATCTCTCTT GTGTCCCTGCCTGTTTGCTCCTGGGCTACCCCACAGACCCTGCCGTCTTTGTTTTAGGGTTTGGTTTGAG TTTCTATCTTTATTATTTTTAATGCCTTCCTAGAGGTTTGGAAATAAAACTTCTTTCCTGAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5708548 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5708548 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19674 same embryo
	EMAGE:19676 same embryo
	EMAGE:19673 same embryo
	EMAGE:19675 same embryo
	EurExpress:euxassay_006104 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source