EMAGE mouse gene expression spatial database

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EMAGE:19676

Fto fat mass and obesity associated ( MGI:1347093)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006122. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006122_01	euxassay_006122_02	euxassay_006122_03	euxassay_006122_04

euxassay_006122_05	euxassay_006122_06	euxassay_006122_07	euxassay_006122_08	euxassay_006122_09

euxassay_006122_10	euxassay_006122_11	euxassay_006122_12	euxassay_006122_13	euxassay_006122_14

euxassay_006122_15	euxassay_006122_16	euxassay_006122_17	euxassay_006122_18	euxassay_006122_19

euxassay_006122_20	euxassay_006122_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19676_wholemount_strong.wlz

19676_wholemount_moderate.wlz

19676_wholemount_weak.wlz

19676_wholemount_possible.wlz

19676_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19676_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8545
Entity Detected:	Fto, fat mass and obesity associated ( MGI:1347093)
Sequence:	sense strand is shown >T8545 GCGGCTTTAGTAGCAGCATGAAGCGCGTCCAGACCGCGGAGGAACGAGAGCGGGAAGCTAAGAAACTGAG GCTCCTTGAGGAGCTTGAAGACACTTGGCTTCCTTACCTGACCCCCAAAGATGATGAGTTCTATCAGCAG TGGCAGCTGAAATACCCTAAACTGGTTTTCCGAGAGGCCGGCAGCATACCAGAGGAGCTGCATAAGGAGG TCCCCGAGGCCTTTCTCACACTGCATAAGCATGGCTGCTTGTTTCGGGACGTGGTGAGGATCCAAGGCAA AGATGTGCTCACCCCAGTGTCTCGCATCCTCATCGGGGACCCAGGCTGCACCTACAAGTACTTGAACACC AGACTCTTCACGGTGCCCTGGCCCGTGAAGGGCTGCACGGTCAAGTACACAGAGGCTGAGATCGCCGCTG CATGTCAGACCTTCCTAAAGCTCAATGACTACCTCCAGGTGGAGACCATCCAGGCCTTGGAAGAACTGGC TGTCAGAGAGAAGGCCAATGAAGACGCTGTGCCACTGTGCATGGCAGAGTTCCCCAGGGCCGGCGTGGGG CCGTCCTGCGATGATGAAGTGGACCTTAAGAGCAGAGCAGCCTACAACGTGACTTTGCTAAACTTCATGG ATCCTCAGAAGATGCCCTACTTGAAAGAGGAGCCCTATTTCGGCATGGGGAAGATGGCGGTGAGCTGGCA TCACGATGAGAACCTGGTGGACAGGTCAGCCGTGGCAGTGTACAGCTATAGCTGCGAAGGCTCTGAGGAT GAAAGTGAGGACGAGTCCAGCTTCGAAGGCAGAGATCCTGATACTTGGCATGTTGGTTTTAAGATCTCTT GGGACATCGAGACACCAGGATTAACAATCCCTCTTCACCAGGGAGACTGCTATTTCATGCTGGATGACCT CAATGCCACCCACCAGCACTGTGTTTTGGCTGGCTCACAGCCTCGGTTTAGTTCCACTCACCGTGTGGCA GAGTGCTCAACAGGCACCTTGGATTATATCTTAGAACGCTGTCAGTTGGCGCTGCAGAATGTCCTCAATG ACTCAGACGATGGCGACGTCTCGTTGAAATCCTTTGATCCTGCAGTTTTGAAACAAGGAGAGGAAATCCA TAATGAGGTGGAGTTTGAGTGGCTGAGGCAGTTCTGGTTTCAAGGCAATCGATACAAACTTTGCACCGAT TGGTGGTGTGAGCCCATGACTCACCTGGAGGGGCTGTGGAAGAAGATGGAGAGCATGACAAATGCGGTGC TCCGTGAAGTTAAAAGAGAGGGGCTCCCGGTGGAACAAAGGAGTGAGATTCTGTCTGCCATCCTGGTCCC GCTCACCGTGCGCCAGAACCTGAGGAAGGAGTGGCATGCCAGGTGCCAGTCCCGAGTCGTCCGGACTTTA CCAGTACAGCAGAAACCAGACTGCCGGCCATATTGGGAGAAGGATGACCCTTCCATGCCTCTGCCCTTTG ACCTCACAGACGTGGTTTCCGAGCTCAGAGGCCAGCTGCTGGAAGCAAGATCCTAGAGGAAGCACAAGTC TTAGGTGGAGAAGGAAAAGATATCCTTGGCTTTCTTCCTCAAACCTTGGACAAGGGTAGTGTAGACTCCT CTCAACCCAGTGGTTATAGGACCTGGGTTCCAATTCAAAACAGCTAGAGAACAAAGCCCACAGATTCTGA AGTGTTGAAACGTTACTGTGTTTTAGTCTGACTTGGTGTTAAATTAGAGCACCCCTCCCTCCCCCCAAAT ATTCTTCACTCACCACCCCCCACACACACCTGGAGCCCTTTTGTCTCCCAAGGCAGCAGTAGAGATGGGC TAAGCCTGTGACAAGCCTCACCTGCTCTCCTCTTTGGCTGGCCACCTCAAAGACCCGGAAGTAGGCCTGG CCATAGGGTGACAGAACTAGAGACATCCATCCCAGCTTCTCACCGTGGCTTCTAGTGGTTAGACCATCCA AGATGGCAGCCAGTGTCTCACCGCGGCTGCACAGATATAAATTCAGCTCAGTTGTCATTAAAACTCCATT CTTCAGTTGCCCTGGCGCATGCTGATGACTGCTGATTTAGACGACACATACAGATGTTTATGTGTTCCAC ATCTGTGAGCGAGCCTTCCTTCTCCCAGCCTTCTGTAGTGGCTAAAACATGGAGGAAAACAGTGAAAGGC AGTCACCACTGTCACCTCTTTCCTCCTCTGAGAGGGAAGGGGCCATGAGCCCTGTGCACTGCACACAGGA AGAGGAAGGAGCTACAAGGAGACAGCCAAACCCTGTCTTTGCGGGGCTTCCCTGATCCAGTGACAGAGAC CAGCCCTGGGACCACTGTTCTGTGGAGCTAGTGTTTCTCCTAGAATTCCCCACTCATAGATACTGACAGG TCGTATGGGGGCTTGAAGCACCCAGTTCCCAACCCCGTGATGTCAGAGCGTCAGAGAGAGCATGGACTCC GATTTCAAAGTGCCAGGAGTAAGACCCACCGCAGCACTCACTCCATGACCTTTGCATGTCCTTGGGTCTC AGTTTTCTCATCTGTATGATTTTGGGAGGTACCCAAAATGCCAACTCCTAATGTGAAAATGACGTGATAT GACAGAGCTACAGATCTAGAGTAATTTCTAGCATACCGCTGAATATCCGATATGTTCCAGTGTCGTTACG TGAGCTGACCTGGAAGAGGCCGTCTTCCTCCCCCCCCCCCCCCCGGGTCTGCAGTCCTTTGCCGTCTTGT GTCTCGTGGCCCACATCTTTCCCTAGTGACCTGAGGCCTTTTGGTGGCTGAAAGGTTGACAACAGTTAGC CCATGATTCTCTTGTATTGGCCGAGACCTGCAATTGGAAGGACACAACATAGTTGTGGTTTTTGCTGCAT CCAGGATTCCAGTTAAGGTGCCTTCCTGCCTAGAATCCACCATAGAAGCTGCACTCCACCAGCCCCAAGG GCTTCCTGGTGCAGGGACCCAAGGCTCTCAGACTTTGATTTGTTCCCCTTTGTCTCTGAGCCAGGACTTC CTCATCAGTAGAGAGTGAGGTACATTCTCACGGGGTAAAATGGAACAACCTAAGTAACGGTATAGGTGGC TTCATATGTCACAGGCTGGCCCGTCTCCCCATCCCCAGCCATCTGGCTCTGTGGTTTATTAGGCCCCGTG ATATTTCTTTGTTCTATTTAATTGGGTGCCAGAATTAAAAATTGAGAGATTCCACATAAAATAAAAATGT CTGCATTCTCTTTAGAAGCCAGAATACCTGTGAGCCCCTGGGTCTCCATAATCGCATGGCGGTGGCTTGG CAATGCCTGTGACCTTTGTGCTCTGCTGTGACCCTGTTGCTCCCTGTCACCATTTTGTAAACCTGCGTCC GTCCCAGCAGCACTTTCTAGAGCTGCTTGCTCGCATCCTTGGTAGAAAGCAGTAGCGTTGAGTGCTCAGT CCCTGCTCCTCCCATTGGTCTAAGTTCATGGTCTGCTTGTATTGCTGTGCTGTGTGCATTTGTTCATACA CAATAAAGAATTAAACGATGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5708558 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5708558 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19672 same embryo
	EMAGE:19674 same embryo
	EMAGE:19673 same embryo
	EMAGE:19675 same embryo
	EurExpress:euxassay_006122 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source