EMAGE mouse gene expression spatial database

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EMAGE:19711

Rcn1 reticulocalbin 1 ( MGI:104559)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006169. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006169_01	euxassay_006169_02	euxassay_006169_03	euxassay_006169_04

euxassay_006169_05	euxassay_006169_06	euxassay_006169_07	euxassay_006169_08	euxassay_006169_09

euxassay_006169_10	euxassay_006169_11	euxassay_006169_12	euxassay_006169_13	euxassay_006169_14

euxassay_006169_15	euxassay_006169_16	euxassay_006169_17	euxassay_006169_18	euxassay_006169_19

euxassay_006169_20	euxassay_006169_21	euxassay_006169_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19711_wholemount_strong.wlz

19711_wholemount_moderate.wlz

19711_wholemount_weak.wlz

19711_wholemount_possible.wlz

19711_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19711_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

gland

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

alimentary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

integumental system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 09 10 11 12

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

pons mantle layer

strong

single cell

strong expression: see section 05

pons ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

spinal cord ventricular layer

strong

regional

strong expression: see section 08 09 10 moderate expression: see section 15

peripheral nervous system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

sensory organ system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 12 13 14 15 16 17 18 19 21 22

cardiovascular system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

urinary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

reproductive system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

respiratory system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

skeleton

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

tail

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8867
Entity Detected:	Rcn1, reticulocalbin 1 ( MGI:104559)
Sequence:	sense strand is shown >T8867 CGCGACCATGGCGCGCGGTGGCCGCTTGGGACTCGCCCTGGGGCTGCTGCTGGCGCTGGTGCTGGCGCTG CGGGCCAAGCCCACGGTGCGCAAGGAGCGCGTGGTTCGGCCGGACTCAGAGCTGGGCGAGCGGCCGCCCG AGGACAACCAGAGCTTCCAGTACGATCATGAGGCCTTCCTGGGCAAGGAGGACTCCAAGACCTTCGATCA GCTAAGCCCGGACGAGAGCAAGGAGAGGCTGGGGAAAATTGTGGATCGAATTGACAGTGATGGTGATGGC CTTGTTACTACTGAGGAGCTGAAACTTTGGATCAAACGGGTACAGAAAAGATACATCTATGATAATGTCG CTAAAGTCTGGAAGGATTATGATAGGGACAAAGACGAAAAGATCTCCTGGGAAGAATACAAGCAGGCCAC CTATGGCTACTACCTGGGAAACCCCGCTGAATTCCATGATAGTTCTGATCACCACACCTTTAAAAAGATG CTGCCACGAGATGAGAGGAGGTTTAAGGCTTCAGACCTTGACGGCGACCTGACAGCTACTCGGGAGGAGT TCACTGCCTTTCTGCACCCAGAGGAGTTTGAACATATGAAGGAGATTGTAGTCCTGGAAACCCTGGAGGA CATCGACAAGAACGGGGATGGTTTTGTGGACCAGGATGAGTACATCGCGGACATGTTTTCCCACGAGGAC AATGGCCCTGAGCCAGACTGGGTTTTGTCTGAACGGGAGCAGTTCAACGACTTCCGAGATCTGAACAAGG ATGGGAAGCTGGACAAGGATGAGATTCGCCACTGGATCCTCCCTCAGGACTACGACCATGCGCAGGCCGA GGCCCGGCATCTGGTGTACGAGTCAGACAAAAACAAGGACGAGATGCTGACCAAGGAGGAGATTCTGGAC AACTGGAACATGTTTGTGGGAAGCCAAGCTACCAACTACGGGGAAGACCTGACCAAAAATCATGATGAAC TTTGAAAGACACTGGTTCTGGTGTGGCCAACTGTCGCAGGCTGTCTGCAGGTGTAGTGGGTGATTGCTAT AGTTGTCAAGGGTATAGCAGTTGTGTCCCTTAACAGTGGGATTATACCTCAGATTGGGGTTAAAAATTAT TTTTCACTCCATATTTACTGGGAAATAACCATCATTATTTATTCCAGTAGGATTTCCCGTGAAGCACATT AAACTCCTGGCAAATTGCAGTCCTCTGAGGGGATACATTGCTACAGCATGATTTTTCTAGACTCCTCTCC CCCACTTAACTCATTAATGGGGAAGAGACCATGAAAGGGCTCTGTAACTCAAGGTGGGTGGGTGGGACAC TGTACAGAGTAACCGCTGTGTATTTGAACTATTAAATTTTGGGGTTTTTAGTTGCCACTGTTAAACGGTG GTGAAGGTAAAGGCTGAAGGAATCAATTTGTATTGTCCTTGGAGCAGGCATGGTATTTCAAATCTTCTAT ACTTGGAGCTGTCTTGTAAATGGTATATAAACTTTTGTGCGTTTTCCTGTATAGACCTGCTAATGTGCAC AATAAACCCACATCCTGGGGTTTTTGTTTTGTTTTTAACACTAAGAAGCAATCAAAAAAAGATAATGTGA ACAAAAACGGAATTTTAGGGATAGGAAAAAGATGGATGCTAGGTATTTGAAAAACTGTATGACCATGGTC AACACCTTAATATACTTGAGAATTTTTTTTACATGTTAATGAGGTGGGTCTGTTTTTTTAAATAGTGAAT GGAAATGCAGTCCCTCCCTGGCTGTGAGGCTAGGGATCAGATGTAGTGGATTTATTATTTGTTGAGTCCT GAATCTCTGAGCCTGCTTCTAGCACAGCTTATGCCAAGGAAACAGGGCAAGGTGAACATCTCAGCCATTG ACCAAACTAGCTGGAGTAACACTTACAGGTGAAATTCCACCCTGTAGATGGGAGGAAAGTGAATAAAGGA TGCCTTTGAGGATCTAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6414128 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6414128 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19716 same embryo
	EMAGE:19715 same embryo
	EMAGE:19714 same embryo
	EMAGE:19712 same embryo
	EMAGE:19713 same embryo
	EurExpress:euxassay_006169 same experiment
	MGI:4827666 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source