EMAGE mouse gene expression spatial database

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EMAGE:19714

Wnt7a wingless-related MMTV integration site 7A ( MGI:98961)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006200. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006200_01	euxassay_006200_02	euxassay_006200_03	euxassay_006200_04

euxassay_006200_05	euxassay_006200_06	euxassay_006200_07	euxassay_006200_08	euxassay_006200_09

euxassay_006200_10	euxassay_006200_11	euxassay_006200_12	euxassay_006200_13	euxassay_006200_14

euxassay_006200_15	euxassay_006200_16	euxassay_006200_17	euxassay_006200_18	euxassay_006200_19

euxassay_006200_20	euxassay_006200_21	euxassay_006200_22	euxassay_006200_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19714_wholemount_strong.wlz

19714_wholemount_moderate.wlz

19714_wholemount_weak.wlz

19714_wholemount_possible.wlz

19714_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19714_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8896
Entity Detected:	Wnt7a, wingless-related MMTV integration site 7A ( MGI:98961)
Sequence:	sense strand is shown >T8896 CCCGCGCCTCAAACACTTGCCGCGATCGCTGGCGCGCAGCGGCGCCCCTTGTTGCGCTTGTTCTCCCCTC CTCTGGCTCCGCGGCTCCCGCGCTCTGGGACAGTCTCCAGTGCCTAGCGCGGACCGACGCACCGACGGAC CGCCCAGGGAGCCTCGGCCCGCGCCCCCTGCGCAGGCTATGTGGATTGCCCCGCCGGGCCCGGCTGGCGG GATCAGCACAGCCCGGCCCGTGGCACCCGCCACCAGCGGGGACTATGACCCGGAAAGCGCGGCGCTGCCT GGGCCACCTCTTTCTCAGCCTGGGCATAGTCTACCTCCGGATCGGTGGCTTCTCTTCGGTGGTAGCTCTG GGTGCGAGCATCATCTGTAACAAGATCCCAGGCCTGGCTCCCAGACAGCGGGCAATCTGCCAGAGCCGGC CGGACGCCATCATCGTCATAGGAGAAGGCTCCCAAATGGGCCTGGACGAGTGTCAGTTTCAGTTCCGAAA TGGCCGTTGGAACTGCTCAGCGCTGGGAGAGCGTACTGTCTTCGGGAAGGAGCTCAAAGTGGGGAGTCGG GAGGCTGCCTTCACCTATGCGATTATCGCTGCGGGCGTGGCCCATGCCATCACTGCTGCCTGCACCCAGG GCAACCTGAGCGACTGTGGCTGCGACAAGGAGAAGCAAGGCCAGTACCACCGGGACGAGGGCTGGAAGTG GGGTGGCTGCTCTGCCGACATCCGCTACGGCATCGGCTTCGCCAAGGTCTTCGTGGATGCCCGGGAGATC AAGCAGAATGCCCGGACGCTCATGAACTTACACAATAACGAGGCGGGTCGGAAGATCCTGGAGGAGAACA TGAAGCTGGAGTGTAAGTGCCATGGTGTGTCAGGCTCCTGTACCACTAAGACGTGCTGGACCACACTGCC ACAGTTCCGAGAGCTAGGCTACGTGCTCAAGGACAAATACAACGAGGCCGTCCACGTGGAGCCTGTGCGT GCCAGTCGAAACAAGCGGCCCACCTTTCTGAAGATCAAGAAGCCCCTGTCCTACCGCAAGCCCATGGACA CTGACCTGGTGTATATCGAGAAGTCACCCAATTACTGTGAAGAGGACCCAGTGACAGGCAGCGTGGGTAC CCAGGGCCGAGCCTGCAATAAGACAGCCCCTCAGGCCAGTGGCTGTGACCTCATGTGCTGTGGCCGTGGC TACAACACACACCAGTACGCCCGGGTGTGGCAGTGCAACTGCAAATTCCACTGGTGCTGCTACGTCAAGT GTAACACGTGCAGCGAGCGCACGGAGATGTATACGTGCAAGTGAATGCGGTCACAGGTCAGATCACAGGC AGGATACAGTTTCCCTGCAGGCCACTGCCTGGATGCTCACAGGGAAAGAACCACAGAAGCACTGTCCTTG TCTTTTCTGCTGAGGGGGGAGGGGTATTCTGGGTTTCCTGCAGACTCCCGTGGGAAGCATCTCTCAGAGG CCCGCCCATTCTTCTCCACATGGATGCTGCTCAGCCACCCTCCCCCAGACACCGCCCGAGCCTCTCCAGG GCTGGAACAAAGTTTTCTACGGCAGGAGCTCTGGAGCCTCGGGCCTCGTCATAGCAATATTTAACAGTTT ATTCTGATATGAGATAATATTAATTTATTTAATTAAAGAGAATTCTTCCACTTCGTCGGGATCCGTCTTC TGCAATCAAAGTGGACTGCTTGAGGTCCTGGTGGGATGACTTGCTAGGACTGGGAGCTGAGAACAGCTGT ACATAATTATTCTTTATGCAGATGTTTCTACTAGTTGATTTCACAAGTACCCTTCTGCAGCGCTAGGTGT TAAGTACAAAGAGAAGACGGTCTTTATACACATATAGATATATATATGCATACACATTTGTAACTTTGTT TTGTTTTGTTTTTGCTGTTTGCTGCTACCTATCCAGACTCTAAGCTGGTCCAGATCTGGAATTGTTTTTC TCCAGGACGTGCTCCTATCCTTTTGCCCTTTACAGTTCAAACCTCTCCGTTAGAAAAGTTCCATTGGGAA TGGCGTGTGTGTGATGGGGACGAGGATCACAAATTCCCAGCAGTTTCCATCCTGAAACGTGAACCACTGG ATAAGAGGCTTTCTAAGAGACTATTTTTCTATGGATATTTTATTTATATGGAGTCTGCCTGCGGTGCCCC ATGGCCCATGCCTCTTCTTAACACTGGTACTCACTCAGGGGCAGAAGGACAAGGCCAGGTGTGTGGGCAG GTCCCCCGGGGACCCTCACACAGCTGGAGCCTGGAGTTCTATTTGCCAAGGGGGCCATAGCAGTTACCAG ATGCCTGGGTTGGGTATCTTCTGTGTTAAACAAGAGGGAACCATCCCCTGGCTTTAGCCTGCTAAGCTCA GGGCTTGGAATGGGGTCACTGGATGGTTATCTTGGGAGATGACCTCTGGATGAGCCTCAGCGGTGGGTCA GTCAGTGTCTCACACACTTTGAGAAGCATGGGACCTGGCATTCATCATCAGGCAGAGGCCAGCTCAGGGA TGCCGCTATCCCATCAGGACAGCCCAGGCACTGCCTCTAGGTGAGGTGTAGTCCTAAGAGAAGGGGTCAA GGAGGGGGAAGGAGGAAGCCAAGGAGTGTTGGCCATCCTCAGTGAAAGCGATGGGAGCGTTCTCTCAGCA GCAGAGACACAGCTGTACCTGTATCTCTCCAATGGGAAACCCCTCCAGAAGGCTGGGGATATTTTTTATG TGTTTCCACATGCATTTCCACCTGTGTGCATGTAAGCACATGCGCACACTCCTGTGCCAGCACTCTGCGG CACCTCCAGGGTGCTCACGGGTACATGTGCTTACATGTATCTCTCTGTGCTTGGGAGATCAGACCATGTG CATGGAGCTGTATGCCTGAGCACTTGTGGTCTCAGGGGTTATTTCCAGGTATCTGCATTTGTGGGTGGGG TGCAAGGTAGACAGCAGGGAACTGATTTGATTGTGTTGAGCCACAGTGAGACTGCAACTCTGAACTCTGT CTCCACAGCTGCTGGTGAAACTCAGATGCCTGTGAGACAACAGCCCTGAGCCTCATGGCCCACATGCTGG GAGCCCCTCAGTGTCTAGGTCATGTCCAGTCCCCCACCTGGGTTACATCACGACCAATAAACATGGCTGT ATGGCTGATTTCTTCCCTTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6415801 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6415801 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19716 same embryo
	EMAGE:19711 same embryo
	EMAGE:19715 same embryo
	EMAGE:19712 same embryo
	EMAGE:19713 same embryo
	EurExpress:euxassay_006200 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source