EMAGE mouse gene expression spatial database

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EMAGE:19922

Abca1 ATP-binding cassette, sub-family A (ABC1), member 1 ( MGI:99607)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009354. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009354_01	euxassay_009354_02	euxassay_009354_03	euxassay_009354_04

euxassay_009354_05	euxassay_009354_06	euxassay_009354_07	euxassay_009354_08	euxassay_009354_09

euxassay_009354_10	euxassay_009354_11	euxassay_009354_12	euxassay_009354_13	euxassay_009354_14

euxassay_009354_15	euxassay_009354_16	euxassay_009354_17	euxassay_009354_18	euxassay_009354_19

euxassay_009354_20	euxassay_009354_21	euxassay_009354_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19922_wholemount_strong.wlz

19922_wholemount_moderate.wlz

19922_wholemount_weak.wlz

19922_wholemount_possible.wlz

19922_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19922_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

single cell

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 23

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 moderate expression: see section 12 13 14

telencephalon ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 16 17 moderate expression: see section 05 06 12 13 14 15 18 19 20

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11 12 13

pons ventricular layer

strong

regional

strong expression: see section 07 08 moderate expression: see section 06 09 10 11 12 13 14 15 16 17

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 14 15 16 moderate expression: see section 10 11 12 13

spinal cord

moderate

single cell

moderate expression: see section 08 09 10 11 12 13 14 15 16

spinal cord ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35855
Entity Detected:	Abca1, ATP-binding cassette, sub-family A (ABC1), member 1 ( MGI:99607)
Sequence:	sense strand is shown >T35855 GAAGGACCACTAAGTAACCCCCTCTTCCCAGGGAGAGCAGCTAGCTTGAAATCTCTCGGATACAATCGAT GCGTCTGACCTTTGGGATCCTCACCATATGGGCAAACAATGGGCTTTGCAGGATGAGAGACACCCACTTA AACCTCTGACGATCTCGAATGGTTCATCTCTTCCGTCATTAACCAGTCATGGAAAACAATCAACAAACTC TGCCACGTGAAATATTTTTTCAGACTTTTCTAACCCAAGCTTAATTTGTTTTCATCTGCAAGCAACGTGG AAAAAAATAGTTATTTCACCAGTGCTCAATGTCATCCGTCTTCATACAAAACAAAAATATGAGTAAGTTC CTGTATTGGGCAAAGTTCAAGTGAGCTTTTGCTATCCTTATTTAACTTCTTCTAAAAATTTGGAAGAATA AGATTTCAGATCTATTTCAAATTATGTTTATATAATGTTATGTAATTCATTGCTTGAAAGGAAAAATGAC AAGTTTGATACTAATGTCAACATAGGTTTCTAAAATGATAAGGAAAGTGTGTTTCCAGTAGTTATTGGCA TACCATTCATTTTAGATATCAAAGATACCGAAATCAAAGAAGCATGTCTTTATTTTTCAAAAACAAACTT CCGTTGGCTTTCTCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89461. Forward Primer - name:089461_F_cDNA_Abca1, sequence:GAAGGACCACTAAGTAACCCCC; Reverse Primer - name:089461_N_SP6_cDNA_Abca1, sequence:TGAGAAAGCCAACGGAAGTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19923 same embryo
	EMAGE:19921 same embryo
	EMAGE:19920 same embryo
	EurExpress:euxassay_009354 same experiment
	MGI:4822859 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source