EMAGE mouse gene expression spatial database

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EMAGE:20177

Fam188a family with sequence similarity 188, member A ( MGI:1914210)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012479. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012479_01	euxassay_012479_02	euxassay_012479_03	euxassay_012479_04

euxassay_012479_05	euxassay_012479_06	euxassay_012479_07	euxassay_012479_08	euxassay_012479_09

euxassay_012479_10	euxassay_012479_11	euxassay_012479_12	euxassay_012479_13	euxassay_012479_14

euxassay_012479_15	euxassay_012479_16	euxassay_012479_17	euxassay_012479_18	euxassay_012479_19

euxassay_012479_20	euxassay_012479_21	euxassay_012479_22	euxassay_012479_23	euxassay_012479_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20177_wholemount_strong.wlz

20177_wholemount_moderate.wlz

20177_wholemount_weak.wlz

20177_wholemount_possible.wlz

20177_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20177_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

weak

regional

weak expression: see section 01 02 03 04 05 22 23 24

olfactory cortex marginal layer

weak

regional

weak expression: see section 09 10 15 16

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 19

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 09 18 19

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 17 18 19 moderate expression: see section 07 14 15 16

neural retina

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31360
Entity Detected:	Fam188a, family with sequence similarity 188, member A ( MGI:1914210)
Sequence:	sense strand is shown >T31360 TTGAGAGGAAGGACCCCCGAGGAAGCGGCTAGGATTTCTGGGAGTCCTGCACAGTCTAGTTGCCAAGTGG AACATTCTTCTGCCTTGGCTGTCGAAGAGCTTGGCTTTGAGCGATTTCATGCATTAATTCAAAAAAGATC ATTCAGAACTGTATCAGAATTAAAAGATGCTGTCTTGGACCAGTATTCAATGTGGGGAAACAAATTTGGA GTCTTGCTTTTTCTCTATTCTGTATTACTGACAAAGGGCATTGAAAACATAAAGAATTCAATTGAAGATG CAAATGAGCCTTTGATAGACCCTGTGTACGGGCATGGCAGCCAAAGCTTAATTAACCTCCTACTGACGGG GCATGCAGTATCTAATGTATGGGATGGTGATAGAGAGTGCTCAGGAATGCAACTTCTCGGTATCCATGAA CAAGCAGCTGTAGGATTCTTAACACTAATGGAAGCTTTACGATATTGTAAGGTTGGTTCTTACTTGAAAT CTCCAAAATTCCCTATTTGGATTGTTGGCAGCGAAACTCACCTCACCGTATTTTTTGCCAAGGATATGGC TTTAGTTGCCCCTGAAGCGCCTTCGGAACAAGCCAGAAGAGTTTTCCAAACCTATGATCCGGAAGATAAT GGATTCATAGCTGACTCCCTTCTAGAAGATGTCATGAAAGCCTTGGACCTTGTTTCAGATCCCGAGTATA TAAATCTCATGAAGAATAAATTAGATCCAGAAGGACTAGGAATCATATTGTTGGGTCCATTTCTTCAAGA ATTTTTTCCTGATCAGGGCTCCAGTGGTCCAGAATCTTTTACTGTCTACCACTACAATGGATTGAAGCAG TCAAATTATAATGAAAAGGTAATGTATGTGGAAGGGACTGCAGTGGTTATGGGTTTTGAAGACCCCATGC TACAGACAGACGACACTCCTATTAAACGCTGTCTGCAAACCAAATGGCCATACATTGAGTTACTGTGGAC CACAGACCGCTGTCCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4217391), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22991. Forward Primer - name:022991_F_IRAV55-58_C09_2310047O13Rik, sequence:TTGAGAGGAAGGACCCCC; Reverse Primer - name:022991_R_SP6_IRAV55-58_C09_2310047O13Rik, sequence:AAGGACAGCGGTCTGTGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20178 same embryo
	EMAGE:20179 same embryo
	EMAGE:20175 same embryo
	EMAGE:20174 same embryo
	EMAGE:20176 same embryo
	EurExpress:euxassay_012479 same experiment
	MGI:4824728 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source