Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21137

Mdh2 malate dehydrogenase 2, NAD (mitochondrial) ( MGI:97050)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21137 EMAGE:21137 EMAGE:21137 EMAGE:21137 EMAGE:21137
"Pseudo-wholemount" of euxassay_004049. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004049_01 euxassay_004049_02 euxassay_004049_03 euxassay_004049_04
EMAGE:21137 EMAGE:21137 EMAGE:21137 EMAGE:21137 EMAGE:21137
euxassay_004049_05 euxassay_004049_06 euxassay_004049_07 euxassay_004049_08 euxassay_004049_09
EMAGE:21137 EMAGE:21137 EMAGE:21137 EMAGE:21137 EMAGE:21137
euxassay_004049_10 euxassay_004049_11 euxassay_004049_12 euxassay_004049_13 euxassay_004049_14
EMAGE:21137 EMAGE:21137 EMAGE:21137 EMAGE:21137 EMAGE:21137
euxassay_004049_15 euxassay_004049_16 euxassay_004049_17 euxassay_004049_18 euxassay_004049_19
EMAGE:21137 EMAGE:21137 EMAGE:21137 EMAGE:21137
euxassay_004049_20 euxassay_004049_21 euxassay_004049_22 euxassay_004049_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21137Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21137_wholemount_strong.wlz
21137_wholemount_moderate.wlz
21137_wholemount_weak.wlz
21137_wholemount_possible.wlz
21137_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21137_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5017
Entity Detected:Mdh2, malate dehydrogenase 2, NAD (mitochondrial) ( MGI:97050)
Sequence:sense strand is shown

>T5017
GCGGCCACTGCGCAGAGTGGAGGTCGTTGGAGTCACTCGTCTTCTTGTAGCTCCTGCCAGTAGCTCCGTG
TCCCGCCCGCCCTAGCCATGCTGTCCGCTCTCGCCCGTCCTGCCGGCGCCGCTCTCCGCCGCAGCTTCAG
CACTTCGGCCCAGAACAATGCTAAAGTGGCTGTCCTGGGAGCTTCTGGGGGCATTGGGCAACCCCTTTCA
CTCCTGCTGAAGAACAGCCCCCTAGTGAGCCGCCTGACCCTCTACGATATCGCTCACACACCTGGTGTGG
CAGCAGATCTGAGTCACATTGAGACCAGAGCAAATGTGAAAGGCTACCTTGGACCGGAGCAGTTGCCAGA
TTGCCTCAAAGGTTGTGATGTGGTGGTCATCCCAGCCGGAGTGCCCAGGAAACCAGGAATGACACGGGAT
GACCTGTTCAACACCAACGCTACCATTGTGGCCACCCTGACGGCTGCCTGTGCCCAGCACTGTCCTGAAG
CCATGGTTTGCATCATTGCCAACCCAGTGAACTCCACCATCCCCATCACAGCAGAAGTTTTCAAGAAGCA
CGGTGTGTACAACCCTAACAAGATCTTCGGTGTGACAACCCTTGACATCGTCAGAGCGAACACGTTTGTG
GCAGAGCTAAAGGGTTTGGATCCAGCTCGAGTCAACGTGCCTGTCATTGGCGGCCACGCCGGGAAGACGA
TCATCCCCCTGATCTCTCAGTGTACCCCGAAGGTTGACTTTCCCCAAGACCAGCTGGCCACACTCACCGG
GAGGATCCAGGAGGCTGGCACAGAAGTCGTGAAGGCCAAGGCTGGAGCAGGTTCTGCCACTCTGTCCATG
GCTTATGCTGGAGCCCGCTTTGTCTTCTCCCTCGTGGACGCCATGAACGGGAAGGAAGGAGTCGTTGAGT
GTTCTTTTGTTCAGTCCAAAGAGACGGAATGCACTTACTTCTCTACGCCCTTGCTCTTGGGGAAAAAGGG
CCTGGAGAAGAACCTGGGCATTGGCAAGATCACTCCTTTTGAGGAAAAAATGATTGCCGAGGCTATCCCT
GAGCTGAAAGCCTCCATCAAGAAAGGCGAGGACTTTGTCAAGAACATGAAGTGAGAGGTGTGAGCCTCGA
GCAGCAGCAGCAGCAGCATCCTAACTTATTCAGCATCATGTCTTTGGAACCACTTGAGAATCTAGTTTGC
GTTGATGGAGGGTGTTGAGTCAGCATCAGCATCTCTTCCAAATTATGTCTGGTCTGTTGATAATGACAGT
AAAGCAGGCTCTGATTTTCTTTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5251192 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5251192 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004049 same experiment
 EMAGE:21136 same embryo
 EMAGE:21138 same embryo
 EMAGE:21139 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS