EMAGE mouse gene expression spatial database

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EMAGE:21252

Alox15 arachidonate 15-lipoxygenase ( MGI:87997)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002115. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002115_01	euxassay_002115_02	euxassay_002115_03	euxassay_002115_04

euxassay_002115_05	euxassay_002115_06	euxassay_002115_07	euxassay_002115_08	euxassay_002115_09

euxassay_002115_10	euxassay_002115_11	euxassay_002115_12	euxassay_002115_13	euxassay_002115_14

euxassay_002115_15	euxassay_002115_16	euxassay_002115_17	euxassay_002115_18	euxassay_002115_19

euxassay_002115_20	euxassay_002115_21	euxassay_002115_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21252_wholemount_strong.wlz

21252_wholemount_moderate.wlz

21252_wholemount_weak.wlz

21252_wholemount_possible.wlz

21252_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21252_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

naris

moderate

regional

moderate expression: see section 1 3 4

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 8 9 0 3 4 5 weak expression: see section 7

esophagus

strong

regional

strong expression: see section 9 0 moderate expression: see section 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4628
Entity Detected:	Alox15, arachidonate 15-lipoxygenase ( MGI:87997)
Sequence:	sense strand is shown >T4628 GTTCATCTGCAAGATGGGTGTCTACCGCATCCGCGTCTCCACCGGGGACTCCGTGTACGCGGGCTCCAAC AACGAGGTCTACCTGTGGTTGATCGGACAGCATGGGGAGGCATCTCTCGGGAAGCTGTTCCGACCCTGTC GGAACTCGGAGGCAGAATTCAAGGTGGATGTGTCAGAATACCTTGGGCCACTGCTGTTTGTGAGAGTGCA GAAATGGCATTATCTCAAAGAGGACGCCTGGTTCTGCAACTGGATTTCTGTGAAGGGCCCCGGAGACCAG GGATCGGAGTACACGTTCCCCTGTTACCGATGGGTTCAGGGCACCAGCATCCTGAACCTCCCTGAGGGCA CTGGCTGCACCGTGGTTGAAGACTCTCAAGGCCTGTTCAGGAACCACAGGGAGGAGGAACTGGAAGAAAG GAGGAGTCTGTACAGGTGGGGCAACTGGAAGGATGGCACAATCCTGAACGTGGCGGCAACCAGTATCTCT GACCTCCCTGTAGACCAGCGATTTCGAGAGGACAAAAGACTTGAATTTGAAGCTTCACAGGTTCTGGGGA CAATGGACACCGTTATTAACTTCCCTAAAAACACTGTGACCTGCTGGAAAAGCCTAGATGACTTCAACTA TGTTTTCAAGAGTGGCCACACCAAGATGGCTGAGCGGGTTCGAAACTCCTGGAAAGAGGATGCTTTCTTT GGGTACCAGTTTCTCAATGGTGCTAACCCCATGGTGCTGAAGCGGTCTACTTGTCTCCCTGCCCGCCTGG TATTCCCTCCGGGGATGGAGAAGCTACAGGCCCAGCTGGATGAGGAGCTCAAGAAAGGCACTCTGTTTGA AGCGGATTTCTTCCTTCTGGATGGGATCAAGGCCAATGTCATCCTTTGTAGTCAGCAGTACCTGGCTGCC CCCCTCGTCATGCTGAAGCTGCAGCCCGATGGGCAACTCTTGCCTATAGCCATCCAGCTCGAACTGCCCA AAACTGGGTCTACCCCACCGCCGATTTTCACGCCCTTGGATCCCCCAATGGACTGGCTCCTGGCCAAATG CTGGGTCCGAAGCTCAGACTTACAGCTTCATGAGCTACAGGCTCATCTTCTGAGGGGACACTTGGTGGCT GAGGTCTTTGCTGTGGCCACCATGAGGTGCCTGCCTTCCGTGCACCCTGTTTTTAAGCTTCTAGTTCCTC ACCTACTCTACACCATGGAAATCAATGTCCGGGCCAGGAGCGACCTGATCTCAGAGAGAGGCTTTTTTGA CAAGGTGATGAGCACAGGTGGAGGAGGCCACCTGGATCTTCTCAAGCAAGCTGGAGCCTTCCTGACCTAC AGCTCATTGTGTCCCCCTGATGACTTGGCTGAGCGAGGACTCCTGGATATTGACACTTGCTTCTATGCTA AAGATGCCCTGCAGCTCTGGCAAGTCATGAATCGGTACGTGGTGGGAATGTTCGATCTCTACTACAAGAC CGACCAAGCTGTTCAGGATGACTATGAACTGCAGAGCTGGTGTCAAGAGATCACTGAGATTGGGTTGCAA GGTGCCCAGGACAGAGGCTTCCCCACCTCCCTCCAGTCCCGGGCTCAGGCTTGCCACTTCATCACCATGT GCATCTTCACATGCACTGCGCAGCACTCTTCCATCCATCTTGGCCAGCTGGATTGGTTCTACTGGGTTCC TAATGCACCCTGCACCATGCGGCTGCCACCACCTACCACCAAGGACGCGACGATGGAGAAGCTGATGGCG ACGCTGCCCAATCCTAATCAGTCTACTCTCCAGATAAATGTCGTTTGGCTCCTGGGCAGACGCCAGGCTG TTATGGTTCCCCTGGGCCAGCATTCAGAGGAACACTTCCCAAACCCTGAGGCCAAAGCTGTGCTGAAGAA GTTCAGAGAGGAGCTGGCTGCCTTGGATAAGGAAATTGAGATTCGTAACAAGAGTTTGGACATACCTTAT GAGTACCTGCGGCCCAGCCTGGTGGAAAACAGCGTGGCCATATGACCATCCCCCCTCTCGATTTGTTATT TAATCAAAACCACTCAAGTGCATCCCTGTGCTTGAGCGGTGGCAGTCCTGCCCTCCCAAGCCCCGCCCTC TGTTCTCCTAAGCCCCACCCTCTGCCCCTCCAAGCCCCGCCCTCTGCCTGTGTGGAACCCTCTTTCAAGA GCCCGCTTTCCATAGTCTGCTGTAGTGAACACATTTTGCTTCGGATGCGTTACCCAGAGTGCCACTTTTC ATTCATCCTTTCTGCTTTTCCTTCCCCATAGGGTAAATAATAGCCATGCTCAGGAAAAGCACCCCGGGAA CACAAAAGGGTGATAATATACATTGTTACACATCGTCTATGAATCAAATAGATCTCAAGCCTTAATAGAG TCTAATAACAACAATAAAAGATGTTTTGGGCTAGTGATAGAAAAGAACAGGGACCTAATTATTTTAAATA AAATGTTGCAAAGATTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5005087 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5005087 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002115 same experiment
	EMAGE:21253 same embryo
	EMAGE:21254 same embryo
	EMAGE:21255 same embryo
	EMAGE:21256 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source