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EMAGE:21254

Grp gastrin releasing peptide ( MGI:95833)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21254 EMAGE:21254 EMAGE:21254 EMAGE:21254 EMAGE:21254
"Pseudo-wholemount" of euxassay_002121. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002121_01 euxassay_002121_02 euxassay_002121_03 euxassay_002121_04
EMAGE:21254 EMAGE:21254 EMAGE:21254 EMAGE:21254 EMAGE:21254
euxassay_002121_05 euxassay_002121_06 euxassay_002121_07 euxassay_002121_08 euxassay_002121_09
EMAGE:21254 EMAGE:21254 EMAGE:21254 EMAGE:21254 EMAGE:21254
euxassay_002121_10 euxassay_002121_11 euxassay_002121_12 euxassay_002121_13 euxassay_002121_14
EMAGE:21254 EMAGE:21254 EMAGE:21254 EMAGE:21254 EMAGE:21254
euxassay_002121_15 euxassay_002121_16 euxassay_002121_17 euxassay_002121_18 euxassay_002121_19
EMAGE:21254
euxassay_002121_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21254Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21254_wholemount_strong.wlz
21254_wholemount_moderate.wlz
21254_wholemount_weak.wlz
21254_wholemount_possible.wlz
21254_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21254_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
dorsal grey horn
strong strong
regionalstrong expression: see section 5 6 7 8 9 0 1 2
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 7 8 1 2 3
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4573
Entity Detected:Grp, gastrin releasing peptide ( MGI:95833)
Sequence:sense strand is shown

>T4573
GGCTCTCGAAGCGGCAGTGGCAGAGCTCCTGAGAACCGTCCTCCTGTCGCTGCTCCGTCGGGACAACGCA
CTCTCAGCCTAGTCGAGAGCTCTGAGGGTTTCCCTCCAGGACCATGCGCGGCTCGGAGCTCTCGCTCTTG
CTGTTGGCTCTGGTCCTCTGCCAGGCGCCCCGGGGGCCAGCTGCCCCGGTGTCGACAGGCGCAGGTGGAG
GCACGGTCCTGGCTAAGATGTATCCGCGCGGCAGTCACTGGGCTGTGGGACACTTAATGGGAAAAAAGAG
CACGGATGAATCCCCGTCCCTGTATGCGGCTGACAGAGACGGCCTGAAGGAGCAGCTGAGGGGGTACGTC
CGCTGGGAAGAAGCTGCAAGGGATTTGCTGGACCTCCTAGAAGCTGCTGGGAACCAAAGCCACCAGCCAC
CTCAGCATCCGCCTCTCAGTCTCCAGCCTACTTGGGATCCCGAGGACGGCAGCTACTTTAACGACGTTCA
AACCGCTAAGTTGGTAGACTCTCTGCTCCAGGTTCTCAAGGAAAAGGGAGGAACTGCCAGCTGAAGGGAC
AACGACAAGGGCGGCTTCCAAGGAGCAAAACAAAACCCTTCAAAGACTGCCTTCTGCAAACGTCAACTCT
ACTGGACCATCAACAAGATTTCCTTTGTGCAATTTACTTAACTATTCTCCTTATCTTTCAACCGCAACTA
AACTCGTGATTTTTCGAGCAGCATCTTCTGGTTTGAACTTGTTTCTGTGAACCACTCACTACTTAGAAAG
AGCTTTCCAATGAACGCTTTTTCCAGCTAGGCTTTTTGTTGATTAGATTCAGGGCTCCAATTTGCTACCA
TTCACAATAAAAAGCTTCCGTGAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4984025 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4984025 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002121 same experiment
 EMAGE:21252 same embryo
 EMAGE:21253 same embryo
 EMAGE:21255 same embryo
 EMAGE:21256 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS