EMAGE mouse gene expression spatial database

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EMAGE:21256

Trim31 tripartite motif-containing 31 ( MGI:2385051)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002130. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002130_01	euxassay_002130_02	euxassay_002130_03	euxassay_002130_04

euxassay_002130_05	euxassay_002130_06	euxassay_002130_07	euxassay_002130_08	euxassay_002130_09

euxassay_002130_10	euxassay_002130_11	euxassay_002130_12	euxassay_002130_13	euxassay_002130_14

euxassay_002130_15	euxassay_002130_16	euxassay_002130_17	euxassay_002130_18	euxassay_002130_19

euxassay_002130_20	euxassay_002130_21	euxassay_002130_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21256_wholemount_strong.wlz

21256_wholemount_moderate.wlz

21256_wholemount_weak.wlz

21256_wholemount_possible.wlz

21256_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21256_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

strong

regional

strong expression: see section 1

hindgut

strong

regional

strong expression: see section 7 8 9 0 1 2

rectum

strong

regional

strong expression: see section 7

midgut

strong

regional

strong expression: see section 4 5 6 7 8 9 0 1 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4609
Entity Detected:	Trim31, tripartite motif-containing 31 ( MGI:2385051)
Sequence:	sense strand is shown >T4609 CAGGGAGAGAGGACTGACCATCTGTTTTCTTCTCTTTAGAATATTCCCCATTTATCGCTGACTCTTTTCT GTCATTATTTATTTAACCCCCCCTTCCCTCATTCTCCTCTAATCCTCTCCCTGAGATACTCTTCAACCTC TGCCTCCTGCTTTTTTCTCTCTTACAAGCACCAGGCCTTGGATTTCTGTACTTTCACATCTCAGATTAGC CCTGCTGTTCCATCATGGCAGGCCAACCTCTGGCCTGCCAACTGCAGGAGGAGGTGACCTGCCCCATCTG CATGGAAATTCTACAAGATCCTGTCACCATTGACTGTGGGCACAACTTCTGCCTGCAGTGTATCAGTCAG GTTGGAAAAACGTCAGAAAAGATCCAATGTCCTCTCTGCAAACTCTCTGTGAATAAGAATACGTTCAGGC CCAATAAGCTGCTGGCTAGTCTTGCAGAGAAGATCCAAAGTATGGATCCTGCTGACATCCAAGCAGAAAA GGAAGACTCAAGATGTCAGAGGCACAAGGAAAAGCTACATTACTTCTGCGAGCAGGATGGGGCATTCCTC TGTGTGGTGTGTCGTGACTCCAAGGACCACAAGTCTCACAATGTCACCTTGATAGATGAGGCTGCCCAGA ACTACAAGGTGCAGATTGAGTCACAGGCCCAAGATTTGGGGCAAAAGGACAAGAAAATAATTGAAGAGAA AAAACAAGGTGAAGGGGCAATCTGGGCGTTCAGGGCCCAGGTGGATCTAGAGAAACTAAAGATCCATGAG GAATTCAAGCTCCTGCGCCAGAGACTAGATGAGGAAGAGAGTTTCCTCCTGTCCAGGCTGGACTGGCTGG AGCAGCAGGGAGCCAAGCAGTTGAGACAATATGTCACTGTGACAGAGAAGCAGCTGAACTCTCTAAGGAA GCTCACTAAATCCTTGAAAATAAGGCTGCAATCATCATCCATGGAGCTACTAAAGGACATTAAAGACGCC TTGAGCAGGGGTAAGGAATTTCAGTTTCTCAACCCAAACCCAGTTCCTGAGGACCTGGAGAAAAAATGCA GTGAAGCAAAAGCAAGGCATGAGTCCATCATAAAAACCCTGACAGAACTCAAAGACGATATGAATGCCGA AGGGAAGAGAGATAAAAGCGCATTCATGAACAGCCTGAATAAAGAAGAAAAGGAGAGCTGGAGCCTGTTA CAGAAGAATAATTCAGTGTTGCCCACCTCAGTCCCTGTGACCCTGGACAAGTCCTCAGCTGACCCAGACC TCACCTTTTCTCAGGATCTAAAGAAAGTGACCTTGTATATTGTAGCTGGAAAAGCTTCCAATAGGCAGGC AAAACCTCGACCATTCTACCCTTTCCACTGTGTGAGGGGTTCCCCAGGCCTCTCCTCAGGCCGCCAGGTG TGGGAGGCAGAAATCCGGGGGCCCTCAGGTGGGGCATGCATTGTGGGCGTGGTTACAGAGTTGGCGCGAG GGGCCCAGAGTCAAACCGTGAGCGCGCAGAGCTACATATGGGCACTGCGGATCTCGCCCTCTGGATGCCA GCCATTCACCAATTGTAAAGCCCAGGAGTACCTCCAGGTCTGTCTTAAGAAAGTGGGTGTCTATGTGAAT CATGACTGTGGAGAGGTCGTCTTCTATGATGCCATCACTAGCAAACACATCTATACTTTCCAAACTTCCT TTGACGGGAAGGTCTTCCCCCTTTTCGGGCTCCAAGTTGCCTGCAGCCATATCACCCTGAGCCCTTAGGG CTGCTCGCTTGTGTGTTCCTCAGTCTCTTCCCACCTATCACTCAGGACGGGATGAGACAAATGGTAGAAG TGAAGTGTGGCCCTACTCTGACACTCTACATGCCACACAATCACCTGATGTTTCCTCTCCCTGTCTCCCC CAAGTAGAGAAGACAGTGGAATACCCCCCAAAGACTATACACGAGGTTTTTCAGTGCTGCACTTGGTAGC AGGACCTCAGAAAAGCTTCTGGCTGCACCCTGCTGACACAGGGGTGCAAGGCGCTGTCCAACACATTGGC TGCTGCTTTCTCTACTGTATTACCTGAAACTCTTGTGACTCATCCTTTCCTGAGATCTGAGAACCAGAGG TTGTACAGAGTCGGCCAGTTCTGTGCCAATGCCCCGTTTGTAGATTTGGACAATCTACAATCAAGAATAA AACCTTTTGACATTTCAAAGCAACTTTGTCATCCTCAACAGCTCTGTCTTCTAATTAAAAGCAAAATTGT AAACCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4990292 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4990292 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002130 same experiment
	EMAGE:21252 same embryo
	EMAGE:21253 same embryo
	EMAGE:21254 same embryo
	EMAGE:21255 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source