EMAGE mouse gene expression spatial database

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EMAGE:21291

Myom2 myomesin 2 ( MGI:1328358)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009376. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009376_01	euxassay_009376_02	euxassay_009376_03	euxassay_009376_04

euxassay_009376_05	euxassay_009376_06	euxassay_009376_07	euxassay_009376_08	euxassay_009376_09

euxassay_009376_10	euxassay_009376_11	euxassay_009376_12	euxassay_009376_13	euxassay_009376_14

euxassay_009376_15	euxassay_009376_16	euxassay_009376_17	euxassay_009376_18	euxassay_009376_19

euxassay_009376_20	euxassay_009376_21	euxassay_009376_22	euxassay_009376_23	euxassay_009376_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21291_wholemount_strong.wlz

21291_wholemount_moderate.wlz

21291_wholemount_weak.wlz

21291_wholemount_possible.wlz

21291_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21291_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 1 2 3 4 0 1 2 3 4

upper leg muscle

moderate

regional

moderate expression: see section 2 3 4 5 6 7 8 9 8 9 2 3 weak expression: see section 0 1

diaphragm

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

hand

moderate

regional

moderate expression: see section 4

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 1 2 3 4 2 3

vertebral axis musculature

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

tongue muscle

moderate

regional

moderate expression: see section 0 1 2 3 4 5

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 5 6 7 8 9 weak expression: see section 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36663
Entity Detected:	Myom2, myomesin 2 ( MGI:1328358)
Sequence:	sense strand is shown >T36663 CACTGAAGAGTGTGAAGTTCGGCTTGTCTGTAAGGTTGCAAACACCAAGAGGGAAACTGTTTTCAAATGG CTCAAGGACGATGTTCTTTACGAAACGGAGACACCGCCAGACCTGGAGAAGGGGGTCTGTGAGCTGCTTA TACCCAAGTTGTCTAAGAAGGACCATGGTGAATACAAAGCAACCTTGAAAGATGACAGAGGTCAGGATGT ATCTGTCCTGGAAGTAGGTGGCAAAGTGTACGAGGACATGATCTTAGCCATGAGTCGTGTGTGTGGAGCC TCTGCCTCACCCCTGAAGGTTCTCTGCACTCCAGAGGGCATCCGGCTTCAGTGCTTCATGAAGTACTTCA CAGAGGAAATGAAGGTCAGCTGGTATCACAAAGAGGCCAAGATCTCCTCCAGTGAGCACATGCGGATCGG AGGGAGCGAGGAGATGGCATGGCTGCAGATCTGTGAGCCCACAGAGAAGGACAAGGGGAAATACACGTTT GAGATTTTCGATGGCAAAGACAGCCACCAACGCTCGCTGGACCTCTCTGGACAAGCTTTTGACGAAGCCT ACGCTGAGTTCCAGCAGCTCAAGGCTGCTGCTTTTGCAGAGAAGAATCGCGGCAAGGTGATTGGCGGCTT ACCAGACGTGGTGACCATAATGGAAGGCAAGACCTTGAACCTCACCTGCACTGTGTTTGGAAACCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98174. Forward Primer - name:098174_F_cDNA_Myom2, sequence:CACTGAAGAGTGTGAAGTTCGG; Reverse Primer - name:098174_N_SP6_cDNA_Myom2, sequence:GGGTTTCCAAACACAGTGCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009376 same experiment
	EMAGE:21290 same embryo
	EMAGE:21292 same embryo
	EMAGE:21293 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source